Paraffin-embedded or frozen sections were subjected to hematoxylin (Mayers or Harris formulations), eosin, Alcian blue, nuclear fast red, Sirius red (IHC WORLD, Woodstock, MD), immunohistochemical or immunofluorescence staining as described (Li et al., 2012b (link); Wei et al., 2010 (link)). The use of archived human specimens was approved by the institutional review board. Detailed procedures for histologic and morphometric analyses, as well as a list of primary and secondary antibodies can be found in the Supplemental Experimental Procedures .
Hematoxylin
Manufactured by IHC World
Hematoxylin is a natural dye extracted from the logwood tree. It is a commonly used stain in histology and cytology laboratories to visualize cell nuclei. Hematoxylin stains cell nuclei a blue-purple color, allowing for the identification and examination of cellular structures.
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2 protocols using hematoxylin
1
Histological and Immunostaining Techniques
2
Immunohistochemical Tissue Preparation
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Slide-mounted anterior ethmoid tissues were deparaffinized in xylene (3 x 5 min) and rehydrated in ethanol (100% ethanol, 2×5 min; 95% ethanol, 5 min; 70% ethanol, 5 min) and Nanopure ddH2O (ddH2O, 2×5 min) (Thermo Fisher Scientific; Pittsburg, PA). After staining of each respective antigen described below, tissues were counterstained with hematoxylin (IHC World; Woodstock, MD), dehydrated following the reverse protocol described above, and slide mounted with Permount (ThermoFisher Scientific; Pittsburg, PA). Tissues were evaluated under an Olympus BX43 upright microscope (Olympus Inc.; Pittsburg, PA), imaged with an EOS Rebel T2i digital SLR camera (Canon Inc.; Melville, NY), and processed with Digital Photo Professional version 4.0 software (Canon Inc.; Melville, NY).
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