Dapi labeled nucleus

Formalin-fixed and paraffin-embedded tissues were sectioned at 4 μm. Tissue slices were placed in an EDTA antigen retrieval buffer. The processed sections were stained with primary antibodies as follows: mouse monoclonal Cytokeratin 8 (CK8; GR3181962-4, Abcam, Cambridge, UK; 1:200) antibody and the rat anti-mouse Cytokeratin 5 (CK5; 57u2005, Affinity, Changzhou, China; 1:200) antibody. The secondary antibodies were as follows: mouse anti-rabbit IgG-FITC (K2017, Santa Cruz, CA, USA; 1:200); F (ab’) 2-goat anti-mouse IgG (H+L) Alexa Fluor 594 (GAR48810900223, Thermo Fisher Scientific, Waltham, MA, USA; 1:200); and the blue fluorescence was a DAPI-labeled nucleus (Sigma-Aldrich, St. Louis, MO, USA). Pictures were taken with a digital pathological section scanner (OLYMPUS, VS120-S6-W, Tokyo, Japan).

Formalin-fixed and paraffin-embedded tissues were sliced at 4 μm. Tissue sections were placed in sodium citrate antigen repair buffer. The processed sections were stained with primary antibodies as follows: mouse monoclonal PCNA (56, Santa Cruz; 1:150) antibody, rabbit anti-mouse CCR10 (22071-1-AP, proteintech; 1:150) antibody, and mouse monoclonal Tβ15(271649, Santa Cruz; 1:150) antibody. The secondary antibodies are as follows: mouse anti-rabbit IgG-FITC (K2017, Santa Cruz, CA, USA; 1:200); F (ab’) 2-goat anti-mouse IgG (H+L) Alexa Fluor 594 (GAR48810900223, Thermo Fisher Scientific, Waltham, MA, USA; 1:200); and the blue fluorescence was a DAPI-labeled nucleus (Sigma-Aldrich, St. Louis, MO, USA). Pictures were taken with a digital pathological section scanner (OLYMPUS, VS120-S6-W, Tokyo, Japan).