All fluorescence spectra were recorded with a Horiba Jobin Yvon Fluorolog3 TCSPC spectrofluorophotometer (Bernsheim, Germany) with 1.0 cm quartz cells. UV–Vis spectra were recorded on a UH5300 Hitachi spectrophotometer (Hitachi Europe, Milan, Italy). The pH measurements were made with a Eutech Instruments pH2700 (Landsmeer, The Netherlands).
Uh5300 spectrophotometer
Manufactured by Hitachi
Sourced in Japan
The UH5300 spectrophotometer is a laboratory instrument manufactured by Hitachi. It is designed to measure the absorbance or transmittance of light through a sample across a range of wavelengths. The core function of the UH5300 is to perform spectroscopic analysis of various materials and substances.
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Lab products found in correlation
Avance 3 600 mhz spectrometer, by Bruker (3 mentions)
2998 photodiode array detector, by Waters Corporation (2 mentions)
2707 autosampler, by Waters Corporation (2 mentions)
Waters 2535 hplc, by Waters Corporation (2 mentions)
Waters 1525 hplc, by Waters Corporation (2 mentions)
Ab65390, by Abcam (2 mentions)
Ph 2700, by Thermo Fisher Scientific (1 mentions)
Nova nanosem 630 scanning electron microscope, by Thermo Fisher Scientific (1 mentions)
Fluorolog 2, by Horiba (1 mentions)
Chloroauric acid haucl4 3h2o, by Merck Group (1 mentions)
F 4600 spectrofluorophotometer, by Hitachi (1 mentions)
Milli q, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
S 4800, by Hitachi (1 mentions)
F 4500 fluorescence spectrofluorometer, by Hitachi (1 mentions)
Lc ms 2010a, by Shimadzu (1 mentions)
Avance 400 mhz spectrometer, by Bruker (1 mentions)
Flexanalysis, by Bruker (1 mentions)
100 ftir, by PerkinElmer (1 mentions)
Acquity sqd ms system, by Waters Corporation (1 mentions)
30 protocols using uh5300 spectrophotometer
1
Fluorescence and UV-Vis Spectroscopy Protocol
2
Analytical Instrumentation for Multidisciplinary Research
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Micrometer (Mytutyo, Japan), pH meter (Thermo Fisher Orion Star A211, Vantaa, Finland), UH5300 Hitachi spectrophotometer (Tokyo, Japan), Anthos Zentyth 3100 spectrophotometer (Anthos, GMBH, Austria), iShak TS4 NXT Microplate Incubator Shaker (GC Life Science, Jaipur, India), Cell-IQ™ Series 5.8 cu.ft. High Heat Sterilization CO2 Incubator model MCO-170AICUVDL-PA (PHC Europe, B.V., Nijverheidsweg, The Netherlands), rheometer MFR 2100 (GBC, VIC, Australia), circulating water bath (Lauda Brinkman Ecoline E100, Suite C Marlton, NJ08053, USA), Nova NanoSEM 630 Scanning Electron Microscope (FEI Company, Hillsboro, OR, USA).
3
Protein Adsorption Evaluation of Hydrogel Membranes
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The evaluation of protein adsorption of the hydrogel membranes was assayed using BSA as the protein model. The evaluation protocol employed in this study was adapted from the existing literature [79 (link),127 (link)]. The protein was dissolved (5% w/v) in phosphate-buffered solutions (PBS, pH 7). Batch experiments were carried out by adding 5 mL of the protein solutions to the hydrogel samples (which had a diameter of 1 cm and thickness of 1 mm). The samples were placed in an orbital shaker (200 rpm) at 37 °C, for 24 h. Aliquots of the solution were collected and initial and equilibrium protein concentrations were assayed spectrophotometrically in a UH5300 Hitachi spectrophotometer (Japan) using the Bradford reagent. The amount of protein adsorbed by the hydrogel membranes was calculated using the following equation:
where q (mg/g) is adsorption capacity; C0 and Ce (mg/L) are the initial and equilibrium concentrations of protein in the solution, respectively; V (L) is the solution volume; and m (g) is the hydrogel membrane mass.
where q (mg/g) is adsorption capacity; C0 and Ce (mg/L) are the initial and equilibrium concentrations of protein in the solution, respectively; V (L) is the solution volume; and m (g) is the hydrogel membrane mass.
4
UV-Vis Study of Squaraines-ctDNA Interaction
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A UH5300 Hitachi spectrophotometer and a 1 x 1 cm quartz cuvettes were used to record all the absorption measurements. The UV-Vis spectra of squaraines complexed with ctDNA were measured in the 200-700 nm range. Experiments were carried out by keeping a fixed amount of squaraines, 10 μM, in a total volume of 3 mL and subsequently titrated with increasing concentration (0-77.4 μM) of ctDNA.
5
Spectroscopic analysis of mucin-GNP interactions
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UV-Visible measurements were recorded using a UH5300 Hitachi spectrophotometer. Increasing concentrations of GNPS (ranging 10 µM -200 µM, 10 µM -400 µM and 10 µM -450 µM for GNPs, GNPs-Cys and GNPs-Tga respectively) were added to a constant concentration of PGM (0.05 mg/mL) or BSM (0.15 mg/mL).
Fluorescence spectra were recorded with a Horiba Jobin Yvon Fluorolog2. Emission spectra were recorded in the range of 280-500 nm after excitation at 258 nm. The excitation and emission slits were 6 nm and 10 nm respectively. A constant concentration of mucin (0.1 mg/mL both for PGM and BSM) was analyzed by successive increasing additions of GNPs (ranging 10 µM -350 µM, 10 µM -300 µM and 10 µM -350 µM for GNPs, GNPs-Cys and GNPs-Tga respectively).
Fluorescence lifetimes were obtained using a NanoLED source (297 nm) and a photon counting detector (TBX04). The goodness of the fit was assessed by the chi-squared value of less than 1.2 and a residual trace that was symmetric about the zero axes. The final result is an average of three independent measurements.
Fluorescence spectra were recorded with a Horiba Jobin Yvon Fluorolog2. Emission spectra were recorded in the range of 280-500 nm after excitation at 258 nm. The excitation and emission slits were 6 nm and 10 nm respectively. A constant concentration of mucin (0.1 mg/mL both for PGM and BSM) was analyzed by successive increasing additions of GNPs (ranging 10 µM -350 µM, 10 µM -300 µM and 10 µM -350 µM for GNPs, GNPs-Cys and GNPs-Tga respectively).
Fluorescence lifetimes were obtained using a NanoLED source (297 nm) and a photon counting detector (TBX04). The goodness of the fit was assessed by the chi-squared value of less than 1.2 and a residual trace that was symmetric about the zero axes. The final result is an average of three independent measurements.
6
Quantifying Drug-Mucin Interactions by UV-Vis
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UV-Vis absorption spectra were measured by a UH5300 Hitachi spectrophotometer at room temperature, using quartz cuvettes (1 cm pathway length). The UV measurements were made in the range of 200-400 nm (with the exception of CFTR(inh)-172 where the range was set on 200-500 nm). Spectra of a 0.05 mg/mL mucin solution were recorded in the absence and in the presence of increasing concentrations of drugs (Figure 3. C 7-ACA (a-g) = 0, 5, 10, 15, 20, 25, 30 µM; C ceftazidime (a-f) = 0, 5, 10, 20, 40, 60 µM; C aztreonam (a-g) = 0, 5, 10, 15, 20, 30, 40, 50 µM; C ampicillin (a-g) = 0, 25, 50, 100, 200, 500, 800 µM; C CFTR(inh)-172 (a-g) = 0, 2, 5, 7, 10, 15, 20 µM; C tobramycin (a-h) = 0, 25, 50, 100, 200, 500, 800, 1600 µM; C levofloxacin (a-g) = 0, 1, 2, 3, 4, 6, 8 µM; C rifampicin (a-g) = 0, 1, 2, 4, 8, 12, 20 µM.).
7
Quantifying Blood Lipid Levels
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Total cholesterol and HDL-cholesterol levels, as well as LDL/VLDL levels were measured according to the manufacturer’s protocol, based on enzymatic colorimetric assay, with a commercial test kit from abcam (Cambridge, MA, Canada). The absorbance was read at 570 nm, using a Hitachi UH 5300 spectrophotometer.
8
Differential Polyphenol Profiles of Olive Oils
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Three types of olive oil with different polyphenol contents were used. HP-EVOO (Olivie Plus 30X) and EVOO were purchased from OLIVIE PHARMA (Atlas Olive Oil, Casablanca, Morocco). HP-EVOO has a very high polyphenol content, especially of Tyr and HTyr, compared to regular EVOO. The enrichment of polyphenols is induced naturally by growing a specific type of olive tree in very arid areas (rocky desert, with a summer temperature of 52 °C). ROO is an olive oil that is marketed in food stores across Quebec, Canada. It was chosen because it is virtually polyphenol-free.
Tyr and HTyr were analyzed and certified by the Biotechnology Laboratory of the Faculty of Sciences, Dhar El Mehraz (Fez, Morocco). The total phenolic content of each oil was determined using the Folin–Ciocalteu reagent (OD 750 nm, Hitachi UH5300 spectrophotometer).
Tyr and HTyr were analyzed and certified by the Biotechnology Laboratory of the Faculty of Sciences, Dhar El Mehraz (Fez, Morocco). The total phenolic content of each oil was determined using the Folin–Ciocalteu reagent (OD 750 nm, Hitachi UH5300 spectrophotometer).
9
Bilirubin-Stabilized Gold Nanoclusters
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Bovine serum albumin (BSA) and chloroauric acid (HAuCl4.3H2O) were purchased from Sigma-Aldrich and Shanghai Reagent (Shanghai, China), respectively. Glutaraldehyde, bilirubin, and all other reagents used in the experiment were obtained from Aladdin Reagent (Shanghai, China). Bilirubin (1 mg) was first dissolved with 0.1 M NaOH (0.1 mL) and then diluted with 50 mM phosphate-buffered solution (PBS) (pH 7.4) to 10 mL to obtain a stock solution (171 μM). Chemicals and solvents were all of analytical reagent grade unless otherwise stated. Ultrapure water (18.2 MΩ, Milli-Q, Millipore) was used throughout the experiment.
Fluorescence spectra and UV-vis absorption spectra were measured with a Hitachi F-4600 spectrofluorophotometer and a Hitachi UH-5300 spectrophotometer, respectively. Scanning electron microscopy (SEM) images were obtained from a field emission SEM (Hitachi S-4800). The morphology and size of the gold nanoclusters were characterized by a JEOL 2100 high-resolution transmission electron microscope (HRTEM) operating at an accelerating voltage of 200 kV.
Fluorescence spectra and UV-vis absorption spectra were measured with a Hitachi F-4600 spectrofluorophotometer and a Hitachi UH-5300 spectrophotometer, respectively. Scanning electron microscopy (SEM) images were obtained from a field emission SEM (Hitachi S-4800). The morphology and size of the gold nanoclusters were characterized by a JEOL 2100 high-resolution transmission electron microscope (HRTEM) operating at an accelerating voltage of 200 kV.
10
Chlorophyll Quantification in Rice Leaves
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The chlorophyll content of rice leaves was determined as described previously (Sartory and Grobbelaar, 1984 ; Xiong et al., 2015 ). To extract chlorophyll, a leaf sample (0.1 g) was incubated in a buffer containing 90% ethanol (v/v) for 12 h at 4 °C in darkness. A UH5300 Spectrophotometer (Hitachi, Tokyo, Japan) was used to determine the absorbance of the chlorophyll extract at wavelengths of 645 nm and 663 nm. The mean value of three biological replicates with the SD was used.
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