TIP (1st grade), 85% phosphoric acid (guaranteed reagent), 2-propanol (1st grade), and copper acetate monohydrate (special grade) were purchased from FUJIFILM Wako Pure Chemical Co. Prior to use, TIP was distilled and 2-propanol was both dehydrated and distilled. The other reagents were used as received. The reagents of the tetraalkylammonium hydroxides are listed in Table 1 .
2 propanol
Manufactured by Fujifilm
Sourced in Japan, United States
2-propanol is a chemical compound that functions as a solvent. It is a colorless, flammable liquid with a characteristic odor. 2-propanol is commonly used in various laboratory applications and industrial processes, where its solvent properties are utilized.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Fujifilm (14 mentions)
Ethanol, by Fujifilm (13 mentions)
Acetonitrile, by Fujifilm (10 mentions)
Acetone, by Fujifilm (8 mentions)
Chloroform, by Fujifilm (8 mentions)
Acetic acid, by Fujifilm (6 mentions)
N hexane, by Fujifilm (5 mentions)
Formic acid, by Fujifilm (5 mentions)
Sodium hydroxide (naoh), by Fujifilm (4 mentions)
Xylene, by Fujifilm (4 mentions)
Lc ms grade methanol, by Fujifilm (4 mentions)
Hydrogen peroxide, by Fujifilm (3 mentions)
Sulfuric acid, by Fujifilm (3 mentions)
Sodium chloride (nacl), by Fujifilm (2 mentions)
Sodium acetate, by Fujifilm (2 mentions)
Su 8 3050, by Nippon Kayaku (2 mentions)
Ultrapure water, by Fujifilm (2 mentions)
1 propanol, by Fujifilm (2 mentions)
Potassium chloride (kcl), by Fujifilm (2 mentions)
Nafion solution, by Merck Group (2 mentions)
63 protocols using 2 propanol
1
Synthesis of Tetraalkylammonium Hydroxides
2
Extraction and Analysis of Caffeine and Stimulants
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The columns used in extracting caffeine and stimulants in the autopsy case body fluids and culture media were Bond Elut Certify (Agilent Technologies, Santa Clara, CA, USA).
A solid powder of caffeine from Sigma-Aldrich (Tokyo, Japan) and methamphetamine hydrochloride from Sumitomo Dainippon Pharma Co. (Osaka, Japan) were used as the standard products. Diazepam-d5 (Sigma-Aldrich, Tokyo, Japan) was used as an internal standard (IS). Deionized pre water was generated using the Milli-Q Purification System (Millipore, Bedford, MA, USA) and was used as distilled water.
As the solvent, 0.1 M phosphate buffer was prepared by dissolving 6.8 g KH2PO4 (Wako, Osaka, Japan) in distilled water at pH 6.0 with potassium hydroxide solution.
Then, 1 M acetic acid was prepared by dissolving 30 mL acetic acid (Wako) in 470 mL distilled water and used as a washing solvent. As one of the eluting solvents, 50 mL dichloromethane (Wako, Osaka, Japan), 20 mL 2-propanol (Wako), and 5 mL ammonia aqueous solution (Wako) were mixed to produce dichloromethane:2-propanol:ammonia (10:4:1).
A solid powder of caffeine from Sigma-Aldrich (Tokyo, Japan) and methamphetamine hydrochloride from Sumitomo Dainippon Pharma Co. (Osaka, Japan) were used as the standard products. Diazepam-d5 (Sigma-Aldrich, Tokyo, Japan) was used as an internal standard (IS). Deionized pre water was generated using the Milli-Q Purification System (Millipore, Bedford, MA, USA) and was used as distilled water.
As the solvent, 0.1 M phosphate buffer was prepared by dissolving 6.8 g KH2PO4 (Wako, Osaka, Japan) in distilled water at pH 6.0 with potassium hydroxide solution.
Then, 1 M acetic acid was prepared by dissolving 30 mL acetic acid (Wako) in 470 mL distilled water and used as a washing solvent. As one of the eluting solvents, 50 mL dichloromethane (Wako, Osaka, Japan), 20 mL 2-propanol (Wako), and 5 mL ammonia aqueous solution (Wako) were mixed to produce dichloromethane:2-propanol:ammonia (10:4:1).
3
Lipid Profiling in Plasma and Feces
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Plasma triacylglycerol and total cholesterol levels were determined using a LabAssay™ Triglyceride Kit (Wako) and a LabAssay™ Cholesterol (Wako), respectively. To analyze fecal fatty acids, the total lipids in feces were extracted as described above without adding an internal standard, after which they were saponified using 0.3 N of NaOH (Wako) in 90% MeOH (Wako). The fatty acids were extracted using n-hexane (Wako), and the organic layer was dried using N2 gas. The residues were dissolved in 2-propanol (Wako), and fatty acid concentrations were measured using a LabAssay™ NEFA (Wako).
4
Quantification of CoQ9 and CoQ10 Isomers
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Re-CoQ10, Ox-CoQ10, Re-CoQ9 and Ox-CoQ9 were obtained from Kaneka Corporation (Osaka, Japan). Acetonitrile, 2-propanol, carboxymethyl cellulose (CMC), ethanol, xylene, hematoxylin and eosin were obtained from Wako Pure Chemical Industries, Ltd. (Tokyo, Japan). α-Cyano-4-hydroxycinnamic acid (CHCA) was purchased from Nacalai Tesque (Tokyo, Japan). All other chemicals were obtained from either Wako or Sigma-Aldrich (St. Louis, MO). All reagents were high-performance liquid chromatography (HPLC) grade.
5
Fabrication of Microfluidic Devices
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Hen egg-white lysozyme (>95% purity) was
purchased from Hampton Research (Aliso Viejo, CA, USA). We used lysozyme
without further purification. Sodium chloride, sodium acetate, acetic
acid, glycerol, acetone, and 2-propanol were purchased from Wako Pure
Chemical Industries, Ltd. (Osaka, Japan). Trichloro(1H,1H,2H,2H-perfuluorooctyl)silane
was purchased from Sigma-Aldrich (St. Louis, MO, USA). Polydimethylsiloxane
(PDMS; SILPOT 184 W/C) was purchased form Dow Corning Toray Co., Ltd.
(Tokyo, Japan). We purchased a SU-83010, a SU-83050, and a SU-8 developer
from Nippon Kayaku Co., Ltd. (Tokyo, Japan). Silicon wafers were obtained
from Global Top Chemical (Tokyo, Japan).
purchased from Hampton Research (Aliso Viejo, CA, USA). We used lysozyme
without further purification. Sodium chloride, sodium acetate, acetic
acid, glycerol, acetone, and 2-propanol were purchased from Wako Pure
Chemical Industries, Ltd. (Osaka, Japan). Trichloro(1H,1H,2H,2H-perfuluorooctyl)silane
was purchased from Sigma-Aldrich (St. Louis, MO, USA). Polydimethylsiloxane
(PDMS; SILPOT 184 W/C) was purchased form Dow Corning Toray Co., Ltd.
(Tokyo, Japan). We purchased a SU-83010, a SU-83050, and a SU-8 developer
from Nippon Kayaku Co., Ltd. (Tokyo, Japan). Silicon wafers were obtained
from Global Top Chemical (Tokyo, Japan).
6
Quantification of Islet-like Cell Transcripts
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On day 0 and day 6 of islet-like cell differentiation, cell aggregates were collected with TRIZol reagent (Thermo Fisher Scientific, Waltham, MA, USA). Total RNA was isolated by adding chloroform (Wako) with centrifugation of 15,000× g for 15 min. The 2-propanol (Wako) was added to collected supernatants and centrifuged at 15,000× g for 15 min to make a purified RNA pellet. The RNA pellet was washed with 75% ethanol and dissolved in RNase-free water. The concentration of RNA was measured by NanoDrop (Shimadzu, Kyoto, Japan) and 100 ng of RNA from each sample was reverse–transcribed by Prime ScriptTM Reverse Transcriptase (Takara Bio Inc., Kusatsu, Japan). After reverse transcription, transcribed complementary DNA samples were quantitated by SYBR Green gene expression assays and detected by StepOne Plus (Thermo Fisher Scientific). Target genes primer sequences are shown in Supplementary Table S1 .
7
Lipid Analysis of Fish Oil and Oils
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Fish oil DHA-46 and GNO were provided by Tama Biochemical Co., Ltd. (Tokyo, Japan) and NPO Arcoiris Naturaleza (Matsudo, Chiba, Japan), respectively. Corn oil was purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan). LC/MS grade methanol, 2-propanol, and ultrapure water were purchased from Wako Pure Chemical Industries (Osaka, Japan). OA, lysophosphatidylcholine (18:1), phosphatidylcholine (18:1/18:1), DHA, phosphatidylcholine (22:6/22:6), and sodium formate were purchased from Sigma-Aldrich (St. Louis, MO, USA). AA was purchased from Cayman Chemical (Ann Arbor, Michigan, MI, USA).
8
Magnetite-Catalyzed Dehydrogenation of 2-Propanol
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Magnetite (Kojundo Chemical Laboratory Co., Ltd.) was used as a catalyst for the dehydrogenation of 2-Propanol. The magnetite powder was compacted at 10 MPa to 13 mm (diameter) × 4 mm (height) to obtain pellets. The magnetite pellets were crushed and sieved to obtain the magnetite catalysts (250–710 μm grains). The particle size distribution and SEM image of the magnetite catalyst are shown in Fig. S9. 2-Propanol was purchased from Wako Pure Chemical Industries, Ltd.
9
Analytical-Grade Compound Extraction
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All the used chemicals were of analytical reagent grade, and the solvents were of HPLC grade. NICO, CLD, SDS (90%), triethylamine (TEA), orthophosphoric acid (85%), methanol, 2-propanol, and acetonitrile (HPLC grade) were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
10
Fluorescent DNA Interactions Characterization
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Ethanol, 1-propanol, 2-propanol, and 2-mercaptoEthanol (2-ME; an antioxidant) were purchased from Wako Pure Chemical Co., Ltd. (Osaka, Japan). T4 GT7 bacteriophage DNA (166 kbp, contour length 57 μm) was purchased from Nippon Gene Co., Ltd. (Tokyo, Japan). The fluorescent cyanine dye, YOYO-1 (1,10-(4,4,8,8-tetramethyl-4,8-diazaundecamethylene(bis(4-((3-methylbenzo1,3-oxazol-2-yl) methylidene)-l,4-dihydroquinolinium) tetraiodide) was purchased from Molecular Probes Inc. (Eugene, OR, USA). Plasmid DNA (Luciferase T7 Control DNA, 4331 bp) containing a firefly luciferase gene and a T7 RNA polymerase promoter sequence was purchased from Promega (Madison, WI, USA). The mRNA (Luciferase Control RNA), which is uncapped in vitro-transcribed RNA containing a 30-base poly(A) tail, was used to produce luciferase and was purchased from Promega (Madison, WI, USA). Other chemicals were obtained from commercial sources and were analytical grade.
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