Polyethylene glycol peg

Manufactured by Merck Group

Sourced in United States, Germany, United Kingdom, India, France, Belgium, Poland, China, Italy

Polyethylene glycol (PEG) is a versatile chemical compound that can be used as a component in various laboratory equipment and applications. PEG is a water-soluble polymer with a wide range of molecular weights, which allows for its use in diverse applications. Its core function is to serve as a stabilizing agent, solvent, or dispersant in various laboratory procedures and experiments.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (10 mentions) Gantrez s 97, by Ashland (9 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions) Sodium hydroxide (naoh), by Merck Group (7 mentions) Acetonitrile, by Merck Group (6 mentions) Hydrochloric acid (hcl), by Merck Group (6 mentions) Polyvinyl alcohol (pva), by Merck Group (6 mentions) Ethanol, by Merck Group (6 mentions) Methylene blue, by Merck Group (5 mentions) Methanol, by Merck Group (5 mentions) Bovine serum albumin (bsa), by Merck Group (5 mentions) Pearlitol 50c mannitol, by Roquette (4 mentions) N hydroxysuccinimide, by Merck Group (4 mentions) Chitosan, by Merck Group (4 mentions) Urea, by Merck Group (4 mentions) Polycaprolactone pcl, by Merck Group (4 mentions) Toluene, by Merck Group (4 mentions) Chloroform, by Merck Group (3 mentions) Sodium carbonate na2co3, by Avantor (3 mentions) Phosphate buffered saline (pbs), by Merck Group (3 mentions)

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146 protocols using polyethylene glycol peg

Polyphenylsulfone Composite Synthesis

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Polyphenylsulfone (PPSU) (Radel^® -R5500) was purchased from Solvay Advanced Polymer (Belgium). N-methyl-pyrrolidone (NMP) and polyvinylpyrrolidone (PVP, 10 k) were used without any further purification from Oxford Lab Chem (India). Graphene oxide (GO), bovine serum albumin (BSA), pepsin, potassium dihydrogen phosphate, disodium hydrogen phosphate dehydrate, polyethylene oxide (PEO, MW of 10 kDa), and polyethylene glycol (PEG, MW 35 kDa) were purchased from Sigma Aldrich (USA) and potassium chloride was purchased from Acros Organics (USA). Hydrochloric acid was purchased from Fisher Chemical (UK), sodium lauryl sulfate GRG from Winlab (UK) and polyethylene glycol (PEG, average MWs of 600 Da, 1 kDa, 4 kDa, 10 kDa and 30 kDa, potassium dichromate and sodium azide from Merck (Germany). Deionized water (Milli-Q) with a resistivity of 18.2 MΩ cm was used throughout the experiments (Millipore, USA).

Shukla A.K., Alam J., Alhoshan M., Dass L.A, & Muthumareeswaran M.R. (2017). Development of a nanocomposite ultrafiltration membrane based on polyphenylsulfone blended with graphene oxide. Scientific Reports, 7, 41976.

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Rheological Analysis of Lung Surfactants

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Survanta (minced bovine lung or beractant, AbbVie Inc., 25 mg/mL), Infasurf (chloroform/methanol extract of calf lung lavage or calfactant, ONY Inc., 35 mg/mL), and Curosurf (minced porcine lung surfactant or poractant alpha, Chiesi USA Inc., 80 mg/mL) were purchased from the pharmacy at University of Minnesota Boynton Health Service and stored at 4°C until use. Prior to the rheology measurements, the samples were heated in a water bath to the desired temperature. The weight concentration refers to the phospholipid concentration in physiological saline (0.9% w/v) solution but does not account for the small fraction (< 2% by weight) of LS proteins SP-B and SP-C. Polyethylene glycol (PEG) of mean molecular weight of 10 or 20 kDa, was purchased from Sigma Aldrich. 20 kDa PEG was mixed with Curosurf and Infasurf at 5% w/v, while Survanta at 1% w/v (immiscible at higher concentration) for the rheology experiments, while 10 kDA PEG was mixed with all three suspensions at 5% w/v for the SAXS experiments. Previous work showed there were minimal differences in surfactant behavior or depletion forces over this range of PEG molecular weight ^{42 (link)}. The mixtures were vortexed for about 30 seconds until the PEG crystals completely dissolved.

Ciutara C.O, & Zasadzinski J.A. (2021). Bilayer Aggregate Microstructure Determines Viscoelasticity of Lung Surfactant Suspensions. Soft matter, 17(20), 5170-5182.

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DPPC and DPPG Lipid Membrane Characterization

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Lipids were obtained from Avanti Polar Lipids, Inc (AL, USA). The solutions of 1,2dipalmitoyl-sn-glycero-3-phosphocholine (DPPC; synthetic purity >99%, 734 g mol -1 ) and 1,2-dipalmitoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt) (DPPG; synthetic purity >99%, 745 g mol -1 ) were prepared in chloroform (Sigma Aldrich UK, 99%+) to a concentration of 0.5 mg mL -1 . PAMAM G5 (~28,800 g mol -1 , ethylenediamine core), PAMAM G4.5 (~26,300 g mol -1 , ethylenediamine core) and Polyethylene glycol (PEG, 20,000 g mol -1 ), the phosphate salts and solvents were obtained from Sigma Aldrich (Dorset, UK). Sodium chloride was obtained from Fisher Scientific UK Ltd (Loughborough, UK). Aqueous Phosphate buffer solution (20 mM) at pH 7 was prepared in house with UHQ water at 18.2 mΩ (ELGA purelab). Enriched salt buffer was prepared adding 144 mM Sodium chloride to phosphate buffer pH 7.

Wilde M., Green R.J., Sanders M.R, & Greco F. (2017). Biophysical studies in polymer therapeutics: the interactions of anionic and cationic PAMAM dendrimers with lipid monolayers. Journal of drug targeting, 25(9-10).

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Analytical Reagents and Standards for LC-MS

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LC–MS‐Grade water, methanol (MeOH), acetonitrile (ACN), formic acid (FA), and acetic acid (HOAc) for the electrospray solvent were purchased from Fisher Scientific (Nazareth, PA, USA). Caffeine was also purchased from Fisher Scientific (Hampton, NH, USA). Theophylline, riboflavin, glutathione (GSH), benzoic acid, sorbic acid, MRFA (peptide), and polyethylene glycol (PEG) were purchased from Sigma Aldrich (St. Louis, MO, USA). Homo‐glutathione (hGSH) was purchased from Bachem (Bubendorf, Switzerland). Stable isotope‐labeled glutathione (SIL‐GSH, ¹³C₂¹⁵N) and stable isotope‐labeled Caffeine (SIL‐Caffeine, ¹³C₃) were purchased from Cambridge Isotope Laboratories, Inc (Tewksbury, MA, USA). Splash Lipidomix Mass Spec Standard (Splashmix) was purchased from Avanti Polar Lipids (Birmingham, AL, USA). Well plates (100 μl) were purchased from Brand GMBH & CO KG (Wertheim, Germany).

Knizner K.T., Bagley M.C., Pu F., Elsen N.L., Williams J.D, & Muddiman D.C. (2022). Normalization techniques for high‐throughput screening by infrared matrix‐assisted laser desorption electrospray ionization mass spectrometry. Journal of Mass Spectrometry, 57(6), e4869.

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Sequence-Coded Poly(Phosphodiester) Synthesis

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Sequence-coded poly(phosphodiester)s were synthesized using iterative phosphoramidite protocols as described in previous publications. [27, 29] In this approach, two slightly different phosphoramidite monomers are used as a molecular binary code. Some of the digital poly(phosphodiester)s have been synthesized manually on crosslinked polystyrene resins [27] and others automatically on porous glass supports. [29] Poly(phosphodiester)s with pending groups were synthesized using two phosphoramidite monomers containing either alkyne or triisopropylsilyl-protected alkyne side groups to allow control on side-chain information by a simple post-polymerization modification method. [30] Poly(alkoxyamine phosphodiester)s have been synthesized using an orthogonal strategy that employs successively phosphoramidite and radical-radical coupling steps. [31] Poly(phosphodiester)s containing inter-byte alkoxyamine spacers were prepared on a DNA synthesizer using porous glass as support. [32] Polymer samples (a few mg) subjected to electrospray ionization (ESI) were first solubilized in methanol (SDS, Peypin, France) and then diluted (1/10 to 1/1000, v/v) in methanol supplemented with ammonium acetate (Sigma Aldrich) at a 3 mM concentration level.
Poly(ethylene glycol) (PEG) and poly(methylmethacrylate) (PMMA) used as internal standards for accurate mass measurements were from Sigma Aldrich.

Amalian J.A., Al Ouahabi A., Cavallo G., König N.F., Poyer S., Lutz J.F, & Charles L. (2017). Controlling the structure of sequence-defined poly(phosphodiester)s for optimal MS/MS reading of digital information. Journal of mass spectrometry : JMS, 52(11).

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Polymer-based Transdermal Adhesive Formulation

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Gantrez® S-97, a copolymer of methyl vinyl ether and maleic acid, (PMVE/MA, molecular weight 1,500,000 Da) was a gift from Ashland, Kidderminster, UK. Poly(ethylene glycol) (PEG; molecular weight 10,000 Da) was purchased from Sigma-Aldrich, Dorset UK. All other chemicals used were of analytical reagent grade. Elastoplast® Invisible Protection plasters were obtained from Beiersdorf UK Ltd., Birmingham, UK.

Quinn H.L., Hughes C.M, & Donnelly R.F. (2017). In vivo and qualitative studies investigating the translational potential of microneedles for use in the older population. Drug Delivery and Translational Research, 8(2), 307-316.

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Cy5-labeled DNA Microarray Protocol

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PPRE\mutant elements from Oka et al. (2012) (link) were prepared as previously reported (Glick et al., 2016a (link)). DNA primers were synthesized (IDT), hybridized to a Cy5-labeled primer and extended using Klenow fragment (exo-) (New England Biolabs) to produce Cy5-labeled dsDNA. Cy5-labeled dsDNA oligonucleotides were diluted to a final concentration of 2 μM then serially diluted in 32 dilutions ranging from 2 μM down to 0.0156 μM. Each sample contained 0.125% Poly ethylene glycol (Peg, Sigma-Aldrich) and 1.25 mg/ml D-trehalose dihydrate (Sigma-Aldrich) in dH₂O, preventing irreversible binding of the DNA to the printed slide as well as for visualization during alignment of the device to the DNA array. A negative control sample with no DNA was included. The oligonucleotides were spotted onto epoxy coated glass substrates (CEL Associates) with a MicroGrid 610 (Bio Robotics) microarrayer using SMT-S75 silicone pins (Parallel Synthesis, USA). Column and row pitch corresponded to the specific device. The microfluidic device that was used contains 64 columns and 64 rows with a pitch of 280 μm by 560 μm, respectively.

Naiman S., Huynh F.K., Gil R., Glick Y., Shahar Y., Touitou N., Nahum L., Avivi M.Y., Roichman A., Kanfi Y., Gertler A.A., Doniger T., Ilkayeva O.R., Abramovich I., Yaron O., Lerrer B., Gottlieb E., Harris R.A., Gerber D., Hirschey M.D, & Cohen H.Y. (2019). SIRT6 Promotes Hepatic Beta-Oxidation via Activation of PPARα. Cell reports, 29(12), 4127-4143.e8.

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Synthesis and Characterization of WBPUU Inks

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WBPUU inks were synthesized with a soft segment (SS) composed by two di-functional polyols, polycaprolactone (PCL) (CAS: 24980-41-4) and poly (ethylene glycol) (PEG) (CAS: 25322-68-3) (Mn = 2000 and 1000 g mol⁻¹, respectively), both provided by Sigma-Aldrich. On the contrary, 2,2-bis(hydroxymethyl) propionic acid (DMPA, 98% purity, CAS: 4767-03-7) and ethylene diamine (EDA, 99% purity, CAS: 107-15-3), which were used as an internal emulsifier and a chain extender, respectively, also provided by Sigma-Aldrich, and isophorone diisocyanate (IPDI, 99.5% purity, CAS: 4098-71-9), supplied from Covestro, were used as components of the hard segment (HS). Dibutyltin dilaurate (DBTDL, CAS: 77-58-7), provided by Sigma-Aldrich, was used as a catalyst. The polyols and the DMPA were dried under a vacuum at 60 °C for 4 h prior to their use. Butanone (supplied by Panreac, CAS: 78-93-3) was used to adjust the viscosity of the prepolymer in an intermediate state as well as to transfer the neutralized DMPA into the reaction medium. The neutralization of the carboxylic groups of DMPA has been carried out by using triethylamine (TEA, 99.5% purity, CAS: 121-44-8), provided by Fluka. Cellulose nanocrystals (CNC) provided by the University of Maine (2018-FPL-CNC-117) were used and CaCl₂ solution (0.1 M, CAS: 10043-52-4) was provided by Sigma-Aldrich.

Vadillo J., Larraza I., Calvo-Correas T., Martin L., Derail C, & Eceiza A. (2022). Enhancing the Mechanical Properties of 3D-Printed Waterborne Polyurethane-Urea and Cellulose Nanocrystal Scaffolds through Crosslinking. Polymers, 14(22), 4999.

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PEG-based UV Spectroscopy Protocol

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Polyethylene glycol (PEG) with average relative molecular weight of 600 was purchased from Sigma-Aldrich, (Steinheim, Germany). All other solvents and chemicals used were purchased at analytical grade from Merck (Darmstadt, Germany). UV spectra were recorded on UV-1800 Shimadzu spectrophotometer (Tokyo, Japan) (λ_max in nm).

Al-Rifai A., Aqel A., Al-Warhi T., Wabaidur S.M., Al-Othman Z.A, & Badjah-Hadj-Ahmed A.Y. (2017). Antibacterial, Antioxidant Activity of Ethanolic Plant Extracts of Some Convolvulus Species and Their DART-ToF-MS Profiling. Evidence-based Complementary and Alternative Medicine : eCAM, 2017, 5694305.

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Qualitative Analysis of Compounds

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Ethanol, ethyl acetate, toluene and formic acid were purchased from Merck (KGaA, Darmstadt, Germany). Polyethylene glycol (PEG) and anisaldehyde were from Sigma-Aldrich (Steinheim am Albuch, Baden-Württemberg, Germany). Additionally, 2-aminoethyl diphenylborinate (NTS) was purchased from Fluka (Steinheim am Albuch, Baden-Württemberg, Germany). All solvents used for extraction, for mobile phase preparation and plate derivatization were of analytical purity grade.

Cobzac S.C., Olah N.K, & Casoni D. (2021). Application of HPTLC Multiwavelength Imaging and Color Scale Fingerprinting Approach Combined with Multivariate Chemometric Methods for Medicinal Plant Clustering According to Their Species. Molecules, 26(23), 7225.

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