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Folin ciocalteu s reagent

Manufactured by Carlo Erba
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Sourced in Serbia

Folin–Ciocalteu's reagent is a colorimetric reagent used for the quantitative determination of phenolic and certain other aromatic compounds. It is composed of phosphomolybdic and phosphotungstic acids. When the reagent is mixed with the sample, the phenolic compounds reduce the reagent, causing a blue color to develop, which can be measured spectrophotometrically.

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Lab products found in correlation

Formic acid, by Thermo Fisher Scientific (3 mentions) Gallic acid, by Merck Group (2 mentions) Acetonitrile, by Thermo Fisher Scientific (2 mentions) Chitosan, by Thermo Fisher Scientific (1 mentions) Coumaric acid, by Merck Group (1 mentions) Acros organics, by Thermo Fisher Scientific (1 mentions) Butylhydroxytoluene bht, by Merck Group (1 mentions) Chlorogenic acid, by Merck Group (1 mentions) Sinapic acid, by Merck Group (1 mentions) Caffeic acid, by Merck Group (1 mentions) Ferulic acid, by Merck Group (1 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions) Epicatechin, by Extrasynthese (1 mentions) Gentisic acid, by Extrasynthese (1 mentions) Protocatechuic acid, by Extrasynthese (1 mentions) Sinapic acid, by Extrasynthese (1 mentions) Syringic acid, by Extrasynthese (1 mentions) Vanillic acid, by Extrasynthese (1 mentions) Vanillin, by Extrasynthese (1 mentions) Catechin, by Extrasynthese (1 mentions)

4 protocols using folin ciocalteu s reagent

1

Antioxidant and Biopolymer Characterization

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In this study, absolute ethanol (Sani-Hem doo, Novi Becej, Serbia), Folin-Ciocalteu’s reagent (Carlo Erba Reagents, Val de Reuil, France), calcium chloride dihydrate, methanol, acetic acid (Zorka Pharma, Sabac, Serbia), gallic acid (purity 97%) (Merck, Darmstadt, Germany), chlorogenic acid, caffeic acid, coumaric acid, ferulic acid, sinapic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), butylhydroxytoluene (BHT) (Sigma Aldrich, St. Louis, MO, USA), sodium salt of alginic acid—very low viscosity (Chem Cruz, Dallas, TX, USA), chitosan molecular weight from 100,000 to 300,000 (Acros Organics, Thermo Fisher Scientific Geel, Belgium) were used. Other used chemicals were p.a. grade (pro analysis).
Savic I.M., Savic Gajic I.M., Milovanovic M.G., Zerajic S, & Gajic D.G. (2022). Optimization of Ultrasound-Assisted Extraction and Encapsulation of Antioxidants from Orange Peels in Alginate-Chitosan Microparticles. Antioxidants, 11(2), 297.
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2

Organic Black Quinoa Phytochemical Analysis

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Dehulled seeds of organic black quinoa (Chenopodium quinoa Willd.), labelled as originating from Bolivia (South America) and packed in a protective atmosphere, were purchased in a retailer specialized in organic and biodynamic products in Italy. Proximate composition per 100 g was, as reported in the nutrition label: 380 kcal energy; 6.2 g fat, of which 0.7 g saturates; 65 g carbohydrates, of which 4.0 g sugars; 9.0 g fibre; 12 g protein; and 0.01 g salt.
Folin–Ciocalteu’s Reagent, methanol, ethanol, acetone, acetic acid and acetonitrile were purchased from Carlo Erba Reagents (Milan, Italy).
Caffeic acid, (+)-catechin, o-coumaric acid, p-coumaric acid, (−)-epicatechin, 3,4-dihydroxycinnamic acid, t-ferulic acid, gallic acid, gentisic acid, 4-hydroxybenzoic acid, protocatechuic acid, rutin, sinapic acid, syringic acid, vanillic acid, and vanillin were purchased from Extrasynthèse (Geney, France) and Sigma-Aldrich (St. Louis, MO, USA).
HPLC grade solvents and water purified by a Milli-Q system (Millipore Corp., Billerica, MA, USA) were used in HPLC analysis.
Melini V, & Melini F. (2021). Modelling and Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Black Quinoa by Response Surface Methodology. Molecules, 26(12), 3616.
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3

Antioxidant Properties of Horgoš Sweet Peppers

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The fruits of the sweet red peppers (C. annuum) cultivar “Horgoš sweet 6” were purchased from a local farmer in Bački Petrovac, Serbia. Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) and TPTZ (2,4,6-Tris(2-pyridyl)-s-triazine) were purchased from Acros Organics (Fair Lawn, NJ, USA), DPPH (2,2-diphenyl-1-picrylhydrazyl) was purchased from Sigma Aldrich (Darmstadt, Germany), gallic acid and Folin–Ciocalteu’s reagent were purchased from Carlo Erba Reagents S.A.S. (Val de Reuil Cedex, France), HPLC grade methanol was purchased from J.T. Baker (Gliwice, Poland). Citric acid monohydrate, potassium metabisulfite, HCl, acetone, NaOH, FeCl3·6H2O, sodium carbonate anhydrous (p.a. > 99%), sodium acetate trihydrate (p.a. > 99%) were of analytical grade.
Lučić M., Potkonjak N., Sredović Ignjatović I., Lević S., Dajić-Stevanović Z., Kolašinac S., Belović M., Torbica A., Zlatanović I., Pavlović V, & Onjia A. (2023). Influence of Ultrasonic and Chemical Pretreatments on Quality Attributes of Dried Pepper (Capsicum annuum). Foods, 12(13), 2468.
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4

Evaluation of Antioxidant and Antidiabetic Properties

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In this study, absolute ethanol (Sani-Hem doo, Novi Bečej, Serbia), Folin–Ciocalteu’s reagent (Carlo Erba Reagents, Val de Reuil, France), calcium chloride dihydrate, methanol, acetic acid (Zorka Pharm, Šabac, Serbia), gallic acid (purity of 97%) (Merck, Darmstadt, Germany), α-glucosidase, 4-nitrophenyl α-D-glucopyranoside (PNP-G), acarbose, 2,2-diphenyl-1-picrylhydrazyl (DPPH), butylhydroxytoluene (BHT), 3-(4,5-dimethylthiazol-2-yl)-2,5-dyphenyl tetrazolium bromide (MTT), phosphate-buffered saline (PBS), dimethyl sulfoxide (DMSO), sodium dodecyl sulfate (SDS), RPMI 1640 nutrient medium, simulated gastric fluid (pH 1.2, SGF), and simulated intestinal fluid (pH 7.4, SIF) without enzyme (Sigma-Aldrich, St. Louis, MO, USA), alginic acid sodium salt (very low viscosity), water (LC-MS grade), acetonitrile (LC-MS grade), formic acid (Thermo ScientificTM, Waltham, MA, USA), chitosan (molecular weight from 100,000 to 300,000) (Acros Organics, Thermo Fisher Scientific, Geel, Belgium) were used. Other used chemicals were pro analysis quality.
Boškov I.A., Savić I.M., Grozdanić Stanisavljević N.Đ., Kundaković-Vasović T.D., Radović Selgrad J.S, & Savić Gajić I.M. (2024). Stabilization of Black Locust Flower Extract via Encapsulation Using Alginate and Alginate–Chitosan Microparticles. Polymers, 16(5), 688.
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