Sigma 50 mm f2.8 dg macro

1maging of oxygen (O₂) distributions in rooted soil of PV and PQ was accomplished by color ratiometric planar optode imaging according to Larsen et al. (2011) . The O₂-optodes used in our study were based on a O₂-quenchable platinum(II)octaethylpor-phyrin (PtOEP) fluorophore (Borisov, 2018 ). O₂-optodes were deployed in parallel, at different regions of interest, to the dual-layer DGT probes in the corresponding PV and PQ replicates. After ~ 1 h incubation with deployed O₂-optodes in the growth room, the rhizotrons were transferred to a dark room and positioned on a horizontal glass plate perpendicular to a digital single lens reflex camera (Canon EOS 1000D, Canon Inc., Tokyo, Japan) equipped with a macro lens (SIGMA 50 mm F2.8 DG MACRO, Sigma Corporation, Kanagawa, Japan). Optical filters and excitation LEDs were used as described in Larsen et al. (2011) . The camera settings were ISO: 100, Av: f5.6, and Tv: 1/30 s. Details on O₂-optode fabrication and calibration can be found in the Supporting Information.

We placed between 10 and 20 ants in the terrarium on the day of capture and allowed them to move freely. Each filmed ant was removed, killed, and weighed to the nearest 0.1 mg (Table 1). For the pit treatment (T7), we transferred an antlion larva to the terrarium at least 1 day before filming. Ants and antlions were used only once. The camera, a Phantom V9.1 with a Sigma 50 mm F 2.8 DG Macro, was positioned perpendicular to the horizontal plane and filmed from above, at 100 frames/s. The image definition was 1632× 1200 pixels. The image encompassed the pit in the pit treatment.