Fmoc arg pbf wang resin

Unless other stated, all chemicals were purchased as the highest purity commercially available and were used without further purifications. 5-(4-carboxyphenyl)-10,15,20-triphenylporphy-rin (PS) was purchased from PorphyChem (Dijon, France). The Fmoc-Arg(Pbf)-Wang resin, the Fmoc-Asp(OtBu)-Wang resin, 9-fluorenyl-methoxy-carbonyl (Fmoc)-amino acids and N,N,N′,N′-Tetramethyl-O-(1H-benzotriazol-1-yl) uronium hexafluorophosphate) (HBTU) were purchased from Iris Biotech GmbH (Marktredwitz, Germany). The N-hydroxysuccinimide, N,N′-dicyclohexylcarbodiimide, N-methylmorpholine (NMM), N-methylpyrrolidinone (NMP), N-ε-maleimidocaproic acid, trifluoroacetic acid (TFA) and triisopropylsilane (TIPS) were purchased from Alfa Aesar (Haverhill, MA, USA). Sodium hydroxide (NaOH; 99.99%), hydrochloric acid (HCl; 36.5%−38%), phosphate-buffered saline (PBS; tablet), dimethyl sulfoxide (DMSO; >99.5%) and Fmoc-Lys-OH HCl were purchased from Sigma-Aldrich (St Louis, MO, USA), Traut’s reagent (2-iminothiolane hydrochloride; >97.0%) was purchased from Alfa Aesar. Ellman’s reagent, 5,5-dithio-bis-(2-nitrobenzoic acid) (>98%), was purchased from Thermo Fisher Scientific (Waltham, MA, USA). AGuIX particles were purchased from NH TherAguix (Lyon, France). All products were used without further purification. Only Milli-Q water (ρ>18 MΩ⋅cm) was used for the aqueous solution preparation.