Male and female mice 6-10 week old were injected i.v. with 400 μl of K/BxN serum at T=0 and blood was obtained at 10 or 20 min for analysis by flow cytometry with the following antibodies: anti-C3 (cat# 0855500, MP Biomedical Life Sciences), anti-Gr1 (cat# 108425; BioLegend); anti-Ly6G (cat# 127654, Biolegend, San Diego, CA); anti-CD11b (cat# 101212; BioLegend ), anti-CD 11a (cat#101008, Biolegend, San Diego, CA); anti-CD162 (PSGL-1, cat# 555306; BD Pharmingen). In general, 106 cells were blocked with the anti-FcR mAb 2.4G2, stained with the indicated Abs for 20 min at 4°C and then washed and resuspended for FACS analysis. Flow cytometry was performed on the BD FACSCalibur™. Data analysis was performed using BD CellQuest™ Pro software. For two-photon (2P) microscopy mice were injected with 200 μl of K/BxN serum at T=0 and 24 h later they were boosted with a s.c. injection of 40 μl of K/BxN serum into one hind foot as previously described (9 (link)). The animals were then subjected to 2P microscopy between 15-60 min after s.c. injection.
Anti cd162 psgl 1
Manufactured by BD
Sourced in United States
The Anti-CD162 (PSGL-1) is a laboratory product that detects the presence of the CD162 (also known as P-selectin glycoprotein ligand-1 or PSGL-1) protein. CD162 is a cell surface protein involved in cell-cell interactions. The Anti-CD162 (PSGL-1) product can be used to identify and quantify cells expressing this protein.
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Lab products found in correlation
2 protocols using anti cd162 psgl 1
1
Kinetics of Immune Cell Response in K/BxN Serum Transfer Arthritis
2
Antibody Interference in EV71 Infection Assay
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Human and rhesus macaque astrocytes were pre-incubated for 1 h at 37°C with the following antibodies (50 μg/ml) against EV71 receptors: anti-CD162/PSGL-1 (BD Biosciences, San Jose, CA, USA), anti-annexinA2 (Abcam, Cambridge, UK), anti-DC-SIGN (Abcam), anti-SCARB2/LIMP2 (Novus Biologicals, Littleton, CO, USA), anti-FGF receptor 3 (FGFR3; Abcam), anti-CCR5 (GeneTex, San Antonio, TX, USA), and anti-CCR3 (GeneTex). The cells were then infected with EV71 (MOI = 0.05), washed twice with PBS and collected at different time points p.i. to determine the viral loads. This experiment has been repeated twice.
In the experiment using human purified complement, a low concentration of virus corresponding to an MOI of 0.005 was co-incubated with human purified complement (Cat. No. 204881, Merck Millipore) at concentrations of 5, 25, and 50 μg/ml (based on the physiological concentration in brain tissue; Barnum, 1995 (link); Jongen et al., 2000 (link)) or control buffer for 1 h, and then these mixtures were added to human astrocyte cultures for the observation of CPE. This experiment has been repeated twice.
In the experiment using human purified complement, a low concentration of virus corresponding to an MOI of 0.005 was co-incubated with human purified complement (Cat. No. 204881, Merck Millipore) at concentrations of 5, 25, and 50 μg/ml (based on the physiological concentration in brain tissue; Barnum, 1995 (link); Jongen et al., 2000 (link)) or control buffer for 1 h, and then these mixtures were added to human astrocyte cultures for the observation of CPE. This experiment has been repeated twice.
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