The human hepatoma cell line, Hep G2, was obtained from the Japanese Collection of Research Bioresources Cell Bank (JCRB, Osaka, Japan). Cells were maintained in 10 ml of low glucose Dulbecco's minimal essential medium supplemented with fetal bovine serum (DMEM-FBS, 5.0%; Wako Chemical), penicillin, 100 U/ml, and streptomycin, 100 μg/ml (Wako Chemical, 168-23191). Stock cultures were grown at 37°C in an atmosphere of 5% CO2 and 95% relative humidity.
Dmem fbs
Manufactured by Fujifilm
Sourced in Japan
DMEM-FBS is a cell culture medium that provides the essential nutrients and growth factors required for the optimal growth and maintenance of various cell lines in vitro. It is a combination of Dulbecco's Modified Eagle's Medium (DMEM) and Fetal Bovine Serum (FBS), which together create a versatile and widely-used cell culture system.
Automatically generated - may contain errors
Lab products found in correlation
Hepg2, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Penicillin, by Fujifilm (1 mentions)
Streptomycin, by Fujifilm (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Endothelial cell growth supplement (ecgs), by Merck Group (1 mentions)
Collagenase type 2, by Merck Group (1 mentions)
Egm 2, by Lonza (1 mentions)
Huvecs, by Lonza (1 mentions)
Hhsec, by ScienCell (1 mentions)
Collagenase type 1, by Worthington (1 mentions)
Dynabead, by Thermo Fisher Scientific (1 mentions)
4 protocols using dmem fbs
1
Maintenance of Hep G2 Hepatoma Cells
2
Culturing MDA-MB-231 Breast Cancer Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human MDA-MB-231 cells were obtained from ATCC (Manassas, VA, US). Cells were maintained at 37 °C (5% CO2 in air) in DMEM-FBS (Dulbecco’s modified Eagle’s medium containing 4500 mg/mL of glucose (Wako Pure Chemical Industries, Osaka, Japan) supplemented with 100 units/mL of penicillin, 100 μg/mL of streptomycin, and 10% (v/v) fetal bovine serum (FBS)).
3
Isolation and Culture of Endothelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HUVECs were purchased from Lonza Walkersville Inc. (Walkersville, MD, USA) and cultured in endothelial cell growth basal medium-2 (EBM-2) culture medium supplemented with endothelial cell growth factors (EGM-2; Lonza Walkersville Inc.). Human hepatic sinusoidal endothelial cells (HHSECs) were purchased from ScienCell (San Diego, CA, USA) and cultured (37°C, 5% CO2) in endothelial cell medium (ECM, containing 5% fetal bovine serum (FBS; ScienCell).
Murine thoracic aorta vascular ECs were isolated from mice as previously described.28 (link) Briefly, mice were anesthetized, and the thoraces were opened to expose the heart and lungs. The aorta was dissected out and immersed in 20% FBS dulbecco’s modified eagle medium (FBS-DMEM; Wako, Tokyo, Japan) in the presence of collagenase type II (Sigma-Aldrich, Tokyo, Japan) for 45 min at 37°C. The cells were then collected and cultured with DMEM supplemented with endothelial cell growth supplement (Sigma-Aldrich, Tokyo, Japan). Five days later, the cells were harvested and utilized for further experiments.
Murine thoracic aorta vascular ECs were isolated from mice as previously described.28 (link) Briefly, mice were anesthetized, and the thoraces were opened to expose the heart and lungs. The aorta was dissected out and immersed in 20% FBS dulbecco’s modified eagle medium (FBS-DMEM; Wako, Tokyo, Japan) in the presence of collagenase type II (Sigma-Aldrich, Tokyo, Japan) for 45 min at 37°C. The cells were then collected and cultured with DMEM supplemented with endothelial cell growth supplement (Sigma-Aldrich, Tokyo, Japan). Five days later, the cells were harvested and utilized for further experiments.
4
Isolation of Pulmonary Endothelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Homogenized whole lungs were treated using a solution of type-1 collagenase (400 U/ml) (Worthington, Lakewood, NJ). CD31-positive cells, including pulmonary vascular endothelial cells, were collected with magnetic beads (Dynabeads; Life Technologies, Grand Island, NY) coated with anti-CD31 antibodies (BD Biosciences, San Diego, CA) as follows.[17] (link) The cellular digest was filtered through a sterile 40-µm mesh and washed three times in 10% FBS-DMEM (Wako Pure Chemical Industries, Osaka, Japan). One milliliter of the cell suspension was placed in a tube with the magnetic beads/CD31-antibody complex and rotated for 20 min at 4°C. The bead-bound cells were then washed five times with 10% FBS-DMEM and the isolated cells were used in the following procedures.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!