We measured male plasma alcohol concentrations using an Ethanol Assay Kit (catalog no. ECET100; BioAssay Systems, Hayward, CA, USA) according to manufacturer's protocol. We determined plasma insulin levels post-mortem using the Mouse Insulin ELISA kit (catalog no. EMINS; Thermo-Fisher, Waltham, MA, USA), according to the recommended protocol. We measured levels of IL1B, IL6, and TNFα using commercial ELISA assays (catalog no. KMC0061 and KMC0011; Thermo-Fisher, Waltham, MA, USA and catalog mo. Ab100689 and Ab100747; Abcam, Cambridge, MA, USA). To determine total cholesterol levels, we used the Total Cholesterol Assay Kit (catalog no. STA- 384; Cell Biolab, Inc, San Diego, CA USA), according to the recommended protocol. To compare the levels of low-density and high-density lipoproteins, we used a colorimetric Cholesterol Assay Kit (catalog no. ab65390; Abcam, Cambridge, MA, USA), according to the recommended protocol. We used the Triglyceride Assay Kit (catalog no. ab65336; Abcam, Cambridge, MA, USA) to contrast the abundance of hepatic triglycerides, according to the suggested protocol. To compare the levels of hydroxyproline, we used the Hydroxyproline Assay Kit (catalog no. MAK008; Millipore-Sigma, St. Louis, MO, USA), and followed the recommended protocol.
Ethanol assay kit
Manufactured by BioAssay Systems
Sourced in United States
The Ethanol Assay Kit is a quantitative colorimetric assay that measures the concentration of ethanol in liquid samples. The kit utilizes an enzymatic reaction to produce a colored product, which is then measured using a spectrophotometer.
Automatically generated - may contain errors
Lab products found in correlation
Mouse insulin elisa kit, by Thermo Fisher Scientific (2 mentions)
Cholesterol assay kit, by Abcam (2 mentions)
Hydroxyproline assay kit, by Merck Group (2 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (2 mentions)
Triglyceride assay kit, by Abcam (1 mentions)
Corticosterone elisa kit, by Assaypro (1 mentions)
Oil red o, by Merck Group (1 mentions)
Mda assay kit, by Nanjing Jiancheng (1 mentions)
Infinite m nano, by Tecan (1 mentions)
Countess 2 automated cell counter, by Thermo Fisher Scientific (1 mentions)
Infinite m nano microplate reader, by Tecan (1 mentions)
8 protocols using ethanol assay kit
1
Evaluating Plasma Biomarkers in Male Subjects
2
Ethanol Kinetics in Rat Blood
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Blood ethanol concentrations were measured in another group of rats to avoid perturbing blood pressure and heart rate measurement. Prior to blood withdrawal, the rats were anesthetized using short-term inhalation anesthetic isoflurane (Panion & BF Biotech Inc., Taoyuan, Taiwan). The blood sample of 0.1 mL was withdrawn from the lateral tail vein using heparinized syringes at 30 min, 60 min, 120 min, and 180 min after oral gavage administration on day 1, day 3 and day 8. Blood ethanol levels were determined by an ethanol assay kit available commercially (BioAssay Systems, Hayward, CA); the rate of increase in absorbance at 580 nm was recorded with a microplate spectrophotometer (Epoch™, BioTek Instruments, Inc., Winooski, VT, USA).
3
Alcohol and Hormone Assays in Males
4
Comprehensive Plasma Biomarker Analysis
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Male plasma alcohol concentrations were measured using an Ethanol Assay Kit (catalog# ECET100; BioAssay Systems, Hayward, CA, USA) according to manufacturer’s protocol. Animals were euthanized by cervical dislocation, blood collected postmortem and plasma insulin levels determined using the Mouse Insulin ELISA kit (catalog# EMINS; Thermo-Fisher, Waltham, MA, USA), according to the recommended protocol. Levels of IL1B, IL6, INFg and TNFa were determined using commercial ELISA assays (catalog# KMC0061 and KMC0011; Thermo-Fisher, Waltham, MA, USA and catalog# Ab100689 and Ab100747; Abcam, Cambridge, MA, USA). Total cholesterol levels were determined using the Total Cholesterol Assay Kit (catalog# STA-384; Cell Biolab, Inc, San Diego, CA USA), according to the recommended protocol. The comparative levels of low-density and high-density lipoproteins were determined using a Cholesterol Assay Kit (catalog# ab65390; Abcam, Cambridge, MA, USA), according to the recommended protocol. The levels of hydroxyproline were determined using the Hydroxyproline Assay Kit (catalog# MAK008; Millipore-Sigma, St. Louis, MO, USA), following to the recommended protocol.
5
Edible Alcohol Hepatotoxicity Assessment
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Edible alcohol was obtained from Beijing Red Star Co., Ltd. (Beijing, China). ALT and AST
detection kits were purchased from Roche (Shanghai, China). An MDA assay kit was provided
by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Ethanol assay kit was
supplied by BioAssay Systems (Hayward, CA, USA). Oil red O, HE and other reagents were
purchased from Sigma-Aldrich (Shanghai, China).
detection kits were purchased from Roche (Shanghai, China). An MDA assay kit was provided
by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Ethanol assay kit was
supplied by BioAssay Systems (Hayward, CA, USA). Oil red O, HE and other reagents were
purchased from Sigma-Aldrich (Shanghai, China).
6
Ethanol Exposure in Liver Cells
7
Cell Viability Assay and Ethanol Quantification
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Cell viability was assessed using a Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) following the manufacturer’s instructions. Ethanol-containing phosphate-buffered saline (PBS) or culture medium was obtained at specified time points, and the ethanol concentration was determined using an Ethanol Assay Kit (BioAssay Systems, Hayward, CA, USA) with an Infinite M nano microplate reader (Tecan, Zurich, Switzerland) according to the manufacturer’s instructions.
8
Cell Viability and Ethanol Assay
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Cell viability was measured using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) or the Countess II automated cell counter (Thermo Fisher Scientific), according to manufacturer’s instructions.
Ethanol containing phosphate-buffered saline (PBS) or culture medium was obtained at the indicated time points, and the concentration of ethanol was measured using the Ethanol Assay Kit (BioAssay Systems, Hayward, CA, USA) with an Infinite M nano microplate reader (Tecan, Zurich, Switzerland), according to manufacturer’s instructions.
Ethanol containing phosphate-buffered saline (PBS) or culture medium was obtained at the indicated time points, and the concentration of ethanol was measured using the Ethanol Assay Kit (BioAssay Systems, Hayward, CA, USA) with an Infinite M nano microplate reader (Tecan, Zurich, Switzerland), according to manufacturer’s instructions.
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