Lightcycler 1536 system

Manufactured by Roche

The Lightcycler 1536 system is a high-throughput real-time PCR instrument designed for rapid and efficient nucleic acid amplification and detection. It features a 1536-well plate format and supports a wide range of applications, including gene expression analysis, genotyping, and pathogen detection.

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Lab products found in correlation

Rt2 profiler pcr array data analysis template, by Qiagen (1 mentions) Rt2 profiler pcr array, by Qiagen (1 mentions) Lightcycler 1536, by Roche (1 mentions) Agilent bravo liquid handling platform, by Agilent Technologies (1 mentions)

Spelling variants of this product

LightCycler (1119 citations) LightCycler system (466 citations) LightCycler 1536 (5 citations) LightCycler®1536 real-time PCR system (2 citations)

2 protocols using lightcycler 1536 system

Chemokine and Angiogenesis Profiling of Sorted Cells

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Changes in chemokines and receptors or angiogenesis levels were measured with an RT² Profiler PCR Array for mouse chemokines and receptors PAMM-022ZA or mouse angiogenesis PAMM-024ZR (Qiagen, Hilden, Germany). Total RNA was extracted from sorted eGFP⁺ cells from BM. The expression of 86 cytokine and chemokine genes was analysed with the LightCycler 1536 system (Roche Diagnostics, Mannheim, Germany), according to the manufacturer's instructions. The data obtained were analysed with the RT² Profiler PCR Array Data Analysis Template (Qiagen). Data were normalized against five housekeeping genes (Actb, B2m, Gapdh, Gusb, Hsp90ab1), and relative expression levels were calculated by the 2^−ΔΔCt method.
PCR array analyses were performed at the Hopital La Pitié Salpétrière ICM, with a LightCycler 1536 (Roche) and RT² Profiler PCR Arrays.

Castela M., Nassar D., Sbeih M., Jachiet M., Wang Z, & Aractingi S. (2017). Ccl2/Ccr2 signalling recruits a distinct fetal microchimeric population that rescues delayed maternal wound healing. Nature Communications, 8, 15463.

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Quantitative PCR Expression Analysis

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Identical reaction volumes were prepared by the Agilent Bravo Liquid Handling Platform (Agilent Technologies) in a 24 Â 64-well design two times according to Agilent and Roche's recommendations. Each 2 μL reaction mixture contained 8 ng cDNA, 10 pmole gene-specific primers, and 1 μL 2Â qPCRBIO SyGreen Mix (PCR Byosystems, London, UK). The following amplification protocol was used: 95 C for 2 min (activation), 60 cycles of 95 C for 10 s, and 60 C for 10 s, followed by 40 C for 10 s final cooling. Amplification was performed on the LightCycler 1536 System (Roche Applied Science) using 42 gene-specific primers and 2 reference genes. Data were collected and processed using the LightCycler 1536 SW 1.0 software. Curves were analyzed by using dynamic tube and slope correction methods. Relative expression of the analyzed genes was normalized to the mean.

Börzsei D., Priksz D., Szabó R., Bombicz M., Karácsonyi Z., Puskás L.G., Fehér L.Z., Radák Z., Kupai K., Berkó A.M., Varga C., Juhász B, & Pósa A. (2021). Exercise-mitigated sex-based differences in aging: from genetic alterations to heart performance. American journal of physiology. Heart and circulatory physiology, 320(2).

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