Nanospect cttm

SPECT/CT experiments were performed using a dedicated small-animal SPECT/CT scanner (NanoSPECT/CT^TM, Mediso Medical Imaging Systems, Budapest, Hungary) as previously reported [14 (link)]. SPECT/CT scans of the PC-3 PIP/PC-3 flu tumor-bearing mice (n = 2) were acquired at 4 h, 24 h, and 48 h after intravenous injection of [¹⁷⁷Lu]Lu-PSMA-TB-01 (25 MBq, 1 nmol, 100 μL saline containing 0.05% BSA). The SPECT/CT image acquisition of mice lasted approximately 60 min. Inhalation anesthesia using a mixture of isoflurane and oxygen was applied to immobilize mice while scanning. Real-time CT reconstruction used a cone-beam-filtered back-projection and reconstruction of SPECT data was performed using the HiSPECT software (version 1.4.3049, Scivis GmbH, Göttingen, Germany). The data were post-processed using VivoQuant (version 3.5, inviCRO Imaging Services and Software, Boston, MA, USA). A Gaussian post-reconstruction filter (full-width at half-maximum = 1.0 mm) was applied and the scale of activity accumulation was set as indicated on the images (minimum value: 0.5 Bq/voxel; maximum value: 50 Bq/voxel).

PET/CT scans were performed using a small-animal bench-top PET/CT scanner (G8, Perkin Elmer, MA, USA; Additional file 1). Mice were injected intravenously with ¹⁵²Tb-PSMA-617 (10 MBq, 1 nmol, 100 μL, diluted in saline) and anesthetized with a mixture of isoflurane and oxygen for in vivo scans (PET/CT and SPECT/CT). Static whole-body PET scans, 10 min in duration, were performed at 2 and 15 h p.i. of the radioligand, followed by a CT scan of 1.5 min. SPECT/CT studies were performed using a small-animal SPECT/CT scanner (NanoSPECT/CTTM, Mediso Medical Imaging Systems, Budapest, Hungary) (Additional file 1). Tumor-bearing mice were intravenously injected with ¹⁷⁷Lu-PSMA-617 (25 MBq, 1 nmol, 100 μL, diluted in saline). Static SPECT/CT scans, 45 min in duration, were performed at 2 h and 15 h p.i. of the radioligand, followed by a CT scan of 7.5 min. Reconstruction of the acquired data was performed using the software of the scanner in question. All images were prepared using VivoQuant post-processing software (version 3.5, inviCRO Imaging Services and Software, Boston, USA). A Gauss post-reconstruction filter (full width at half maximum = 1 mm) was applied to the images and the scale adjusted by cutting 5% of the lower signal intensity to make the tumors and kidneys readily visible.

SPECT/CT images were obtained using a dedicated small-animal SPECT/CT scanner (NanoSPECT/CT^TM, Mediso Medical Imaging Systems, Budapest, Hungary) as previously reported [21 (link)]. [¹⁷⁷Lu]Lu-Ibu-PSMA-02 (25 MBq, 1 nmol, 100 µL) diluted in saline containing 0.05% BSA was injected in a lateral tail vein of the mouse. SPECT scans of ~45 min duration were performed 4 and 24 h after injection of the radioligand followed by a CT of 7.5 min duration. During the in vivo scans, the mice were anesthetized with a mixture of isoflurane and oxygen. Reconstruction of the acquired data was performed using HiSPECT software (version 1.4.3049, Scivis GmbH, Göttingen, Germany). All images were prepared using VivoQuant post-processing software (version 3.5, inviCRO Imaging Services and Software, Boston, MA, U.S.A.). A Gauss post-reconstruction filter (FWHM = 1.0 mm) was applied to the images and the scale of radioactivity was set as indicated on the images (minimum value = 0.7 Bq/voxel to maximum value = 70 Bq/voxel).

PET/CT scans were performed using a small-animal bench-top PET/CT scanner (G8, Sofie Biosciences, Culver City, California, USA, and Perkin Elmer, Massachusetts, USA) and a small-animal SPECT/CT scanner (NanoSPECT/CT^TM, Mediso Medical Imaging Systems, Budapest, Hungary), respectively (Additional file 1). During the scans, the mice were anesthetized with a mixture of isoflurane and oxygen.
Static whole-body PET scans of 20 min duration were performed at 30 min, 2 and 4 h after injection of ⁴⁴Sc-PSMA-617 (~5 MBq, 1 nmol) and of 10 min duration at 30 min and 2 h after injection of ⁶⁸Ga-PSMA-617 and ⁶⁸Ga-PSMA-11 (~5 MBq, 1 nmol), respectively. The PET scans were followed by a CT of 1.5 min. The SPECT scan of 45 min duration was performed 2 h after injection of ¹⁷⁷Lu-PSMA-617 (~50 MBq, 1 nmol) followed by a CT of 7.5 min. Reconstruction of the acquired data was performed by using the software of the respective scanner.
All images were prepared using VivoQuant post-processing software (version 2.10, inviCRO Imaging Services and Software, Boston USA). A Gauss post-reconstruction filter (full width at half maximum = 1 mm) was applied to the images, which were presented with the scale adjusted to allow visualization of the most important organs and tissues, usually by cutting 0.5–1% of the lower scale.