Total proteins were extracted from seedlings using 1× Laemmli sample buffer (Bio-Rad, 1610737) and immunoblotting was carried out with an HRP-conjugated anti-GFP antibody (Miltenyi Biotec, 130-091-83, 1:3000 dilution) or a Rabbit monoclonal anti-GFP antibody (Cell Signaling Technology, 2956, 1:1000 dilution) followed by anti-rabbit IgG-HRP (Cell Signaling Technology, 7074, 1:3000 dilution). Either cross-reacting bands or proteins stained by Ponceaus S (Sigma-Aldrich, P3504; 0.1% w/v in 5% acetic acid) were used as loading controls. Chemiluminescent substrate (Thermo Fisher Scientific, 34075) was used for signal detection.
Rabbit monoclonal anti gfp antibody
Manufactured by Cell Signaling Technology
Rabbit monoclonal anti-GFP antibody is a laboratory reagent used to detect the presence and localization of green fluorescent protein (GFP) in cells and tissues. It is a highly specific and sensitive tool for visualizing and studying GFP-tagged proteins.
Automatically generated - may contain errors
Lab products found in correlation
Chemiluminescent substrate, by Thermo Fisher Scientific (1 mentions)
Laemmli sample buffer, by Bio-Rad (1 mentions)
Hrp conjugated anti gfp antibody, by Miltenyi Biotec (1 mentions)
Anti rabbit igg hrp, by Cell Signaling Technology (1 mentions)
Mouse anti vimentin monoclonal antibody, by Santa Cruz Biotechnology (1 mentions)
Oil red o, by Merck Group (1 mentions)
Rabbit polyclonal anti ki67 antibody, by Abcam (1 mentions)
Oil red o, by Santa Cruz Biotechnology (1 mentions)
2 protocols using rabbit monoclonal anti gfp antibody
1
Extracting and Analyzing Seedling Proteins
2
Immunohistochemistry and Oil Red O Staining
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IHC staining was performed as previously described 16 (link) by using rat monoclonal anti-CD31 antibody (1:200, Santa Cruz), rabbit polyclonal anti-Ki67 antibody (1:200, Abcam), rabbit monoclonal anti-GFP antibody (1:300, Cell Signaling), mouse monoclonal anti-vimentin antibody (1:100, Santa Cruz), or mouse monoclonal anti-PPARγ2 antibody (1:200, Santa Cruz).
For Oil Red O staining, cells or 10 µm cryosections were fixed by neutral buffered 10% formalin, 30 min at room temperature. After rinsing in 60% isopropanol, cells were stained with Oil Red O (Sigma) at 60˚C for 5 min.
For Oil Red O staining, cells or 10 µm cryosections were fixed by neutral buffered 10% formalin, 30 min at room temperature. After rinsing in 60% isopropanol, cells were stained with Oil Red O (Sigma) at 60˚C for 5 min.
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