All EBOV-infected cells [9 (link)] and mice were handled under maximum containment in a biosafety level (BSL)-4 laboratory at the U.S. Army Medical Research Institute of Infectious Diseases (Frederick, MD, USA). Six-10 week old C57Bl/6 mice were used in experiments and were purchased from the National Cancer Institute (Frederick, MD, USA). Mice lacking perforin, CD4, CD8a, TCRa, IFN-gamma, TNF-alpha, gamma/delta T cell receptor, or B cells (mice lacking IgM heavy chain), as well as CD11c-DTR, CD11b-DTR, and SCID mice, were purchased from The Jackson Laboratory (Bar Harbor, Maine, USA). Mice were infected intraperitoneally (i.p.) with ~1000 pfu (~3,000 LD50) of mouse-adapted EBOV [23 (link)]. This dose was chosen since 1000 pfu is the standard dose of lethal EBOV infection in animal models; our analysis of this particular batch of mouse-adapted EBOV showed that 1000 pfu was ~3,000 LD50.
Cd11b dtr
Manufactured by Jackson ImmunoResearch
Sourced in United States
CD11b-DTR is a transgenic mouse strain that expresses the diphtheria toxin receptor (DTR) under the control of the CD11b promoter. CD11b is a cell surface integrin expressed on myeloid cells, including monocytes, macrophages, and neutrophils. The expression of DTR in CD11b-positive cells allows for the selective depletion of these cell types upon administration of diphtheria toxin.
Automatically generated - may contain errors
7 protocols using cd11b dtr
1
Mouse Model of Lethal Ebola Infection
2
Murine CD11b-DTR and TIM-4 Deficient Models
3
Sirt1 Deficiency in Myeloid Cells
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The study was approved by the UCLA Animal Research Committee (ARC #1999–094). C57BL/6 male mice: wild-type (WT), CD11b-DTR (Jackson Laboratory, Bar Harbor, ME), FLOX and myeloid-specific SIRT1-deficient (mSirt1-KO) (NIEHS, Research Triangle Park, NC) were used. Animals were housed under pathogen-free conditions and received human care outlined in the Guide for Care and Use of Laboratory Animals (National Academies Press, 2011).
4
Immunological Experiments with Genetically Modified Mice
5
Murine Models in Immunological Research
6
Sirt1 Deficiency in Myeloid Cells
7
Immunological Experiments with Genetically Modified Mice
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All animal experiments were approved by the Institutional Animal Care and Use Committee of the University of Illinois. CD11b-DTR, Sphk2−/−, Sting−/− and C57Blk/6J mice breeding pairs were initially obtained from Jackson Laboratory (Farmington, CT, USA) and bred at the University of Illinois at Chicago. Mouse colonies were maintained in a pathogen-free housing facility at the University. All experiments were performed in both male and female that were between 6–8 weeks old.
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