Doxycycline free fetal calf serum

Manufactured by Takara Bio

Doxycycline-free fetal calf serum is a cell culture supplement derived from the blood of bovine fetuses. It provides a source of essential nutrients, growth factors, and other components to support the growth and maintenance of various cell types in vitro.

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Lab products found in correlation

Hemin, by Merck Group (1 mentions) Fetal calf serum (fcs), by Merck Group (1 mentions) Z2 coulter counter, by Beckman Coulter (1 mentions) Gentamycin, by Thermo Fisher Scientific (1 mentions) Hygromycin, by Thermo Fisher Scientific (1 mentions) Neomycin, by Merck Group (1 mentions) Coulter counter z2 particle counter, by Beckman Coulter (1 mentions) Fetal calf serum (fcs), by Gemini Bio (1 mentions)

Close spelling variants of this product

Doxycycline

2 protocols using doxycycline free fetal calf serum

T. brucei Wild-type and Inducible Strains

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Experiments were performed in wild-type procyclic T. brucei brucei 427 strain and 427 cells carrying the machinery necessary for doxycycline inducibility (29-13). The 427 cells were cultured at 27°C in SDM-79 medium supplemented with 7.5 μg/ml hemin and 20% fetal calf serum (Sigma-Aldrich, St. Louis, MO). The 29-13 cells were cultured at 27°C in SDM-79 medium supplemented with 7.5 μg/ml hemin, 20% doxycycline-free fetal calf serum (Clontech, Mountain View, CA), 50 μg/ml hygromycin, and 15 μg/ml neomycin. Cell growth was monitored using a particle counter (Z2 Coulter Counter, Beckman Coulter, Brea, CA; Moxi Z, Orflo, Ketchum, ID).

McAllaster M.R., Ikeda K.N., Lozano-Núñez A., Anrather D., Unterwurzacher V., Gossenreiter T., Perry J.A., Crickley R., Mercadante C.J., Vaughan S, & de Graffenried C.L. (2015). Proteomic identification of novel cytoskeletal proteins associated with TbPLK, an essential regulator of cell morphogenesis in Trypanosoma brucei. Molecular Biology of the Cell, 26(17), 3013-3029.

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Culturing Procyclic Trypanosoma brucei

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Cells used in all experiments were either procyclic T. brucei brucei strain 427 or the doxycycline-inducible strain 29-13. 427-based cell lines were cultured at 27 °C in Beck’s Medium (Hyclone, GE Healthcare, Logan, Utah) supplemented with 500 μg/mL penicillin-streptomycin-glutamine (Hyclone), 10% fetal calf serum (Gemini Bioproducts, West Sacramento, CA), and 10 μg/mL gentamycin (ThermoFisher Scientific, Waltham, MA). 29-13-based lines were cultured at 27°C in Beck’s Medium supplemented with 500 μg/mL penicillin-streptomycin-glutamine, 15% doxycycline-free fetal calf serum (Clontech), 10 μg/mL gentamycin, 50 μg/mL hygromycin (ThermoFisher Scientific), and 15 μg/mL neomycin (Sigma-Aldrich, St. Louis, MO). Cell concentration was determined using a Z2 Coulter Counter particle counter (Beckman Coulter, Brea, CA).

Hilton N.A., Sladewski T.E., Perry J.A., Pataki Z., Sinclair-Davis A.N., Muniz R.S., Tran H.L., Wurster J.I., Seo J, & de Graffenried C.L. (2018). Identification of TOEFAZ1-interacting proteins reveals key regulators of Trypanosoma brucei cytokinesis. Molecular microbiology, 109(3), 306-326.

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