Protein was isolated from cells as described21 (link). Briefly, cell lysates were electrophoresed on 4-20% Pierce Precise Protein Gels (Thermo Scientific) and transferred to nitrocellulose membranes. Membranes were probed with the following primary antibodies: rabbit-anti-ATG16L1 (1:500, Sigma) guineapig-anti-p62 (1:1000, Progen Biotechnik, Heidelberg, Germany), rabbit anti-Caspase-1 (1:5000, Dr. Gabriel Nuñez), rat anti-Caspase-11 (1:250, Sigma), rat anti-NLRP3 (1:250, R&D systems), and rabbit-anti-GAPDH (1:1000, Cell Signaling Technology, Danvers, MA). Goat-anti-rabbit IgG-HRP, goat-anti-guinea pig IgG-HRP, and goat-anti-rat IgG-HRP secondaries (Santa Cruz Biotechnology, Dallas, TX) were used and detected by SuperSignal West Dura (Thermo Scientific).
Goat anti guinea pig igg hrp
Manufactured by Santa Cruz Biotechnology
Goat-anti-guinea pig IgG-HRP is a secondary antibody that binds to guinea pig immunoglobulin G (IgG) and is conjugated with horseradish peroxidase (HRP). This product is used as a detection reagent in various immunoassays and Western blotting techniques.
Automatically generated - may contain errors
Lab products found in correlation
Supersignal west dura, by Thermo Fisher Scientific (2 mentions)
Goat anti rat igg hrp, by Santa Cruz Biotechnology (2 mentions)
Pierce precise protein gels, by Thermo Fisher Scientific (2 mentions)
Rabbit anti atg16l1, by Merck Group (2 mentions)
Guinea pig anti p62, by Progen Biotechnik (2 mentions)
Rabbit anti caspase 1, by Merck Group (2 mentions)
Rat anti caspase 11, by Merck Group (2 mentions)
Rat anti nlrp3, by R&D Systems (2 mentions)
Goat anti rabbit igg hrp, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (2 mentions)
2 protocols using goat anti guinea pig igg hrp
1
Western Blot Analysis of Autophagy Markers
2
Western Blot Analysis of Autophagy Markers
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Protein was isolated from cells as described21 (link). Briefly, cell lysates were electrophoresed on 4-20% Pierce Precise Protein Gels (Thermo Scientific) and transferred to nitrocellulose membranes. Membranes were probed with the following primary antibodies: rabbit-anti-ATG16L1 (1:500, Sigma) guineapig-anti-p62 (1:1000, Progen Biotechnik, Heidelberg, Germany), rabbit anti-Caspase-1 (1:5000, Dr. Gabriel Nuñez), rat anti-Caspase-11 (1:250, Sigma), rat anti-NLRP3 (1:250, R&D systems), and rabbit-anti-GAPDH (1:1000, Cell Signaling Technology, Danvers, MA). Goat-anti-rabbit IgG-HRP, goat-anti-guinea pig IgG-HRP, and goat-anti-rat IgG-HRP secondaries (Santa Cruz Biotechnology, Dallas, TX) were used and detected by SuperSignal West Dura (Thermo Scientific).
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