Dako envision system hrp dab kit

Animals were sacrificed by an i.p. overdose of Nembutal (300 μL, Ceva) and subsequently perfused with 4% ice-cold paraformaldehyde (PFA) solution (Sigma-Aldrich). After overnight postfixation in 4% PFA, brain tissue was stored at 4°C in a PBS solution containing 0.1% sodium azide (Fluka, Sigma-Aldrich). Five μm thick paraffin sections were cut and a Masson trichrome, a Prussian blue, and an Iba1 immunostaining were performed [32 (link)]. For the latter, sections were stained with an Iba1 antibody (1/250). Visualization was achieved by using the Dako EnVision + system-HRP (DAB) kit (Dako, Glostrup, Denmark).

Animals were sacrificed by an intraperitoneal overdose of nembutal (300 μl; Ceva). They were subsequently perfused with a 4 % ice-cold paraformaldehyde (PFA) solution in PBS (Sigma-Aldrich). After overnight post-fixation in 4 % PFA, the brain tissue was stored in a 0.1 % sodium azide solution in PBS (Fluka; Sigma-Aldrich) at 4 °C. Paraffin sections (5 μm) were sliced and a Masson trichrome, a Prussian blue staining and an Iba1 staining were performed [31 (link)]. For the latter, sections were stained with an Iba1 antibody (1/250). Visualization was achieved using the Dako EnVision + system-HRP (DAB) kit (Dako, Glostrup, Denmark).