CUMYL-4CN-BINACA was obtained from Cayman Chemical (MI, USA), and SR141716 from Sigma-Aldrich (NSW, Australia). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Thermo Fisher (MA, USA). Fetal bovine serum (FBS) was obtained from Moregate Biotech (NZ). Cell culture plasticware was sourced from Corning (NY, USA). [3H]-CP 55,940, harvest plates, and Irgasafe plus, were all purchased from PerkinElmer (MA, USA). Polypropylene v-bottom mixing 96-well plates were acquired from Hangzhou Gene Era Biotech Co Ltd (China). Branched polyethyleneimine was purchased from Sigma (MO, USA). The Bradford Protein Assay Kit was purchased from BIORAD (CA, USA).
Branched polyethyleneimine
Manufactured by Merck Group
Sourced in United States, Germany
Branched polyethyleneimine is a polymeric compound used in various laboratory applications. It serves as a versatile reagent with a range of chemical properties and functions. The core function of this product is to facilitate specific laboratory procedures, but a detailed description without interpretation or extrapolation is not available.
Automatically generated - may contain errors
Lab products found in correlation
Glutaraldehyde, by Merck Group (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Sodium hydroxide (naoh), by Merck Group (3 mentions)
Polyvinylpyrrolidone pvp, by Merck Group (2 mentions)
Silver nitrate, by Merck Group (2 mentions)
Tetraethyl orthosilicate, by Merck Group (2 mentions)
Alginic acid, by Merck Group (2 mentions)
Hydrochloric acid (hcl), by Merck Group (2 mentions)
Poly sodium 4 styrenesulfonate, by Merck Group (2 mentions)
Bradford protein assay kit, by Bio-Rad (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Cell culture plasticware, by Corning (1 mentions)
Cumyl 4cn binaca, by Cayman Chemical (1 mentions)
Sr141716, by Merck Group (1 mentions)
Irgasafe plus, by PerkinElmer (1 mentions)
Hek293f, by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by BioConcept (1 mentions)
Pen strep, by PAN Biotech (1 mentions)
Ammonium persulfate, by Merck Group (1 mentions)
Ammonium hydroxide solution, by Merck Group (1 mentions)
33 protocols using branched polyethyleneimine
1
CUMYL-4CN-BINACA Binding Assay
2
Transient Expression of Connexin 32 in HEK293F Cells
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HEK Freestyle 293F (HEK293F; Thermo Fisher Scientific) cells were cultured in 15-cm cell culture dishes containing Dulbecco’s modified Eagle’s medium (DMEM; BioConcept) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (PenStrep; PAN Biotech) with 5% CO2 and cultured at 37°C. At 80% confluence, cell medium was exchanged to DMEM supplemented with 2% FBS and 1% PenStrep. The transfection mixture was prepared with Cx32-YFP-twinStrep plasmid and branched polyethyleneimine (Sigma-Aldrich) at a ratio of 1:2 (w/w) with serum-free DMEM. The transfection mixture was added to cells after 5 min of incubation at room temperature. After 48 hours of expression at 37°C and 5% CO2, the cells were harvested using a cell scraper in buffer A containing 25 mM tris-HCl (pH 8.0) and 150 mM NaCl and collected by centrifugation at 800g and 4°C. The cells were washed with buffer A and collected by centrifugation. The supernatant was discarded, and the cells were frozen at −80°C for further experiments.
3
Synthesis of Polymeric Microspheres for Adsorption
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Styrene (St) (>99%), divinylbenzene (DVB) (80%), sodium 4-vinylbenzenesulfonate (NaVBS) (>90%), ammonium persulfate (NH4)2S2O8 (APS) (>98%), 2,2′-azobis(2-methylpropionitrile) (AIBN) (>98%), ammonium hydroxide solution (NH4·OH) (28%) and basic alumina (Al2O3) (≥98%), 3-(triethoxysilyl)propionitrile (TESPN) (97%), 3-(triethoxysilyl)propyl-methacrylate (TSPM) (99%), sulfuric acid (H2SO4) (95.0–98.0%), branched polyethyleneimine (bPEI, Mn ≈ 10,000 by GPC, Mw ≈ 25,000 by LS) and paraffin wax (mp 53–58 °C) were purchased from Sigma-Aldrich (Buchs, Switzerland). N,N’-diisopropylcarbodiimide (DIC) (99%) was purchased from Acros Organics (Basel, Switzerland). St and DVB were passed through basic alumina to remove the stabilizer before usage. AIBN was purified by re-crystallization twice from methanol and stored at −20 °C before usage. Other reagents were used as received. Ultrapure water (UPW; conductivity c = 0.055 µS/cm and resistivity, ρ = 18.2 MΩ cm at 298 K) was obtained from an Arium 611 VF water purification system (Startorius stedim biotech, Aubagne, France), and it was used as the aqueous medium in all experiments.
4
Functionalization of Cellulose Polymers
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Carboxymethyl cellulose (Mn = 124 × 103 g mol−1, Mw = 333 × 103 g mol−1, D = 2.685; degree of substitution 0.7), hydroxypropyl cellulose (Klucel, Mw = 1.0 × 105 g mol−1 (manufacturer details, no method disclosed), molar substitution (MS) = 5.54 [37 (link)]), rhodamine B isothiocyanate and branched polyethylene imine (50% aqueous solution, Mw = 750 × 105 g mol−1, Mn = 60 × 105 g mol−1 (manufacturer details, no method disclosed)) were purchased from Sigma-Aldrich (unless otherwise mentioned) and used without further purification.
5
Synthesis of Metal Nanoparticles
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Silver nitrate (AgNO3), cupric sulfate pentahydrate (CuSO4·5H2O), potassium tetrachloroplatinate (K2PtCl4, 99.99%), sodium tetrachloropalladate (Na2PdCl4, 98%), branched polyethyleneimine (BPEI, MW = 750,000, 50 wt % solution in water), L-ascorbic acid (C6H8O6, ≥ 99%), and polyvinyl pyrrolidone (PVP, MW = 10,000) were purchased from Sigma-Aldrich. All chemicals were used as received without further purification.
6
Synthesis of Cr(VI) Adsorbing Mesoporous Silica
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All chemicals were obtained from the indicated suppliers and used without further purification. Tetraethyl orthosilicate (TEOS, 99.999% metal basis), hexadecyltrimethylammonium bromide (CTAB H5882, >98%) and branched polyethyleneimine (PEI, MW = 800) were purchased from Sigma-Aldrich. Cr(VI) solutions were prepared by diluting Cr standard solution (Kanto Chemical, Tokyo, Japan) with deionized water. In addition, all vials and tools were cleaned with ethanol three times and deionized water three times before they were used.
7
Biomaterial Characterization and Cell Culture
8
Polymer-based Nano-formulation Preparation
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Methoxyl polyethylene glycol (mPEG) with Mw of 2 kDa, mPEG-NHS (MW = 2000), branched polyethyleneimine (PEI) with Mw of 25 kDa (PEI), indomethacin (IND) were obtained from Sigma (USA). 4-Formylbenzoic was purchased from Aesar Alfar (Ward Hill, MA, USA). Cremophor EL was purchased from BASF Corp (Germany). Docetaxel (DTX) were supplied by Xi’an Xuan Biological Technology Co., Ltd (Xi’an, China). Penicillin, streptomycin, fetal bovine serum (FBS), and Eagle’s minimum essential (EME) medium were purchased from HyClone (Waltham, MA, USA). RPMI1640 medium was obtained from Gibco (USA). All the other reagents are commercially available and used as received.
9
Polymer-Based Nanoparticle Uptake Assay
10
Synthesis and Characterization of Polymeric Particles
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Poly(methyl vinyl ether alt maleic acid) (PMVEMA) with Mw of 216 and 1980 kDa, branched poly(ethyleneimine) (PEI) with Mw of 600–1000 kDa, fluorescein isothiocyanate, rhodamine 6G, and bromoiodomethane were supplied by Sigma Aldrich. Melamine formaldehyde particles with the average size of 5.17 ± 0.09 μm were synthesized by microparticles GmbH.
The PDMS kit, Sylgard 184 – silicone elastomer, contained the monomer and curing agent was obtained by Dow Corning. The standard buffers for calibration and adjustment of pH were obtained by Mettler Toledo.
The PDMS kit, Sylgard 184 – silicone elastomer, contained the monomer and curing agent was obtained by Dow Corning. The standard buffers for calibration and adjustment of pH were obtained by Mettler Toledo.
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