Mil 1α

Manufactured by Thermo Fisher Scientific

Sourced in Japan

MIL-1α is a laboratory instrument designed to detect and quantify the presence of interleukin-1 alpha (IL-1α) in biological samples. It utilizes an enzyme-linked immunosorbent assay (ELISA) method to measure the concentration of IL-1α. The core function of MIL-1α is to provide accurate and reliable data on IL-1α levels, which can be useful in various research and clinical applications.

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Lab products found in correlation

Hil 33, by R&D Systems (1 mentions) Hccl 2, by R&D Systems (1 mentions) Mil 4, by Thermo Fisher Scientific (1 mentions) Mil 1β, by Thermo Fisher Scientific (1 mentions) Mifn γ, by Thermo Fisher Scientific (1 mentions) Golgiplug, by BD (1 mentions)

3 protocols using mil 1α

Cytokine Quantification in Biological Samples

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The concentrations of cytokines hIL-33, hIL-1α, hCCL-2 (R&D systems), hTSLP, mIL-33, mTSLP, mIL-4 and mIL-1α (eBiosciences) in the cell supernatant, Lung homogenate and in the BALF were estimated using paired antibodies following manufacturer’s instructions.

Kale S.L., Agrawal K., Gaur S.N, & Arora N. (2017). Cockroach protease allergen induces allergic airway inflammation via epithelial cell activation. Scientific Reports, 7, 42341.

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BALF Cytokine Measurement in Mice

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BALF were collected from euthanized mice by intratracheal injection and aspiration of 1 ml PBS. Cytokine levels in BALF or serum were measured by ELISA using the following paired antibodies kits: mIL-1α, mIL-1β, mIFNγ (eBioscience), mIL-18 (MBL Nagoya, Japan).

del Barrio L., Sahoo M., Lantier L., Reynolds J.M., Ceballos-Olvera I, & Re F. (2015). Production of Anti-LPS IgM by B1a B Cells Depends on IL-1β and Is Protective against Lung Infection with Francisella tularensis LVS. PLoS Pathogens, 11(3), e1004706.

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Evaluating IL-1 and IFN-γ Responses

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To determine IL-1 responsiveness, mIL-1α (2.5 ng/mouse, eBioscience) was delivered in 0.1 ml PBS by IP injection. Peritoneal cells were processed and stained for flow cytometry. To determine IFN-γ production by NK cells, spleens were made into a single cell suspension and cultured overnight with mIL-12p70 (2 ng/ml, eBioscience) and/or mIL-18 (10 ng/ml, R&D). The next morning, 1× GolgiPlug (BD Biosciences) was added for 4 h. Non-adherent cells were removed and processed for ICS.

Sivick K.E., Arpaia N., Reiner G.L., Lee B.L., Russell B.R, & Barton G.M. (2014). Toll-like receptor deficient mice reveal how innate immune signaling influences Salmonella virulence strategies. Cell host & microbe, 15(2), 203-213.

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