Recombinant mouse tgf β1

Manufactured by Cell Signaling Technology

Sourced in United States

Recombinant mouse TGF-β1 is a protein produced using recombinant DNA technology. It is the mouse homologue of the transforming growth factor beta 1 (TGF-β1) protein, which is a member of the TGF-beta superfamily and plays a role in regulating various cellular processes.

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Lab products found in correlation

Gapdh, by Abcam (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Ecl western blotting substrate, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by GE Healthcare (1 mentions) β actin primary antibody, by Abcam (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Mitosox red superoxide indicator, by Thermo Fisher Scientific (1 mentions) Dab substrate kit, by Abcam (1 mentions) Hyclone fetal bovine serum fbs, by Thermo Fisher Scientific (1 mentions) Acetyl l carnitine, by Merck Group (1 mentions) 4 hne, by Abcam (1 mentions) Senescence β galactosidase staining kit, by Cell Signaling Technology (1 mentions) β glycerophosphate, by Merck Group (1 mentions) Hrp conjugated secondary antibody, by Abcam (1 mentions) Oil red o, by Merck Group (1 mentions) Mab5986, by R&D Systems (1 mentions) Carbon tetrachloride ccl4, by Sinopharm (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

TGF-β1 (107 citations) Recombinant TGF-β1 (6 citations)

6 protocols using recombinant mouse tgf β1

Detailed Cell Culture and Antibody Protocol

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All chemicals were purchased from Sigma–Aldrich unless otherwise noted here. Dulbecco’s Modified Eagle Medium (DMEM) with high glucose was purchased from Hyclone (SH30003.03), and all other cell culture media was purchased from Invitrogen (Carlsbad, CA, USA) unless otherwise indicated. Recombinant mouse TGF-β1 (#5231) was from Cell Signaling Technology. Anti-MBP (ab40390; 1:200 for IF) was purchased from Abcam. GAPDH [ab181602; 1:3,000 for western blot (WB)] was purchased from Abcam. F4/80 antibody was produced via hybridoma cell line HB-198. All cell lines were purchased from American Tissue Culture Collection (ATCC, Manassas, VA, USA). Alexa Fluor 555- and 647-conjugated secondary antibodies (1:1,000 for IF) were purchased from Invitrogen.

Liu Y., Hammel G., Shi M., Cheng Z., Zivkovic S., Wang X., Xu P., He X., Guo B., Ren Y, & Zuo L. (2021). Myelin Debris Stimulates NG2/CSPG4 Expression in Bone Marrow-Derived Macrophages in the Injured Spinal Cord. Frontiers in Cellular Neuroscience, 15, 651827.

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Senescence and Oxidative Stress Analysis

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Recombinant mouse TGF-β1 and Senescence β-Galactosidase Staining Kit were purchased from Cell Signaling Technology (Danvers, MA, USA). MitoSOX™ Red superoxide indicator, L-glutamine, ProlongH Gold antifade reagent with DAPI and DMEM were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). Acetyl-L-carnitine, β-glycerophosphate and Oil Red O were purchased from Sigma-Aldrich (St. Louis, MO, USA). DAB Substrate Kit, PE-conjugated CD44 antibody, FITC-conjugated CD90 antibody, 4-HNE, SOD2, β-actin primary antibodies and HRP-conjugated secondary antibodies were purchased from Abcam (Cambridge, MA, USA). Alkaline phosphatase antibody was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). HyClone Fetal Bovine Serum (FBS) was purchased from Thermo Fisher Scientific Inc. (Cleveland, OH, USA). ECL Western-blotting substrate was purchased from Thermo Fisher Scientific (Rockford, IL, USA). The PVDF membrane was purchased from GE healthcare (Pittssburgh, PA, USA).

Wu J., Niu J., Li X., Wang X., Guo Z, & Zhang F. (2014). TGF-β1 induces senescence of bone marrow mesenchymal stem cells via increase of mitochondrial ROS production. BMC Developmental Biology, 14, 21.

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Comprehensive Molecular Analysis of CCl4-Induced Liver Injury

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Carbon tetrachloride (CCl4) (batch number: 20070721) was obtained from Sinopharm Co, Ltd (Shanghai, China). Safflower yellow pigment A (SYPA), hydroxyl safflower yellow pigment A (HSYPA), and Apigenin (4′,5,7-trihydroxyflavone, flavonoid) were obtained from MCE Corporation (USA). Luteolin (3′,4′,5,7-hydroxyl-flavone) was obtained from Sigma Chemical Co. (St. Louis, MO, USA). Recombinant mouse TGF-β1 was purchased from Cell Signaling Technology (Danvers, MA, USA). CCl₄ and olive oil were provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
Antibodies against COLLAGEN (COL1) (ab34710), CD59 (ab124396), NAGA (ab233264), ITIH3 (ab97758), and DNMT1 (ab19905) were obtained from Abcam (MA, USA). Antibodies against α-SMA (19245S) were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies against CCR1 (MAB5986) were purchased from R&D Systems. Antibodies against KIF23 (A4896) and P4HA3 (A137667) were purchased from ABclonal (Wuhan, China). Antibodies against GAPDH (60004-1-IG) were purchased from Proteintech part, Inc. (Rosemont, USA).

B.a.t.u.d.e.l.i.g.e.n., Han Z., Chen H., N.a.r.i.s.u., Xu Y., A.n.d.a, & Han G. (2023). Luteolin Alleviates Liver Fibrosis in Rat Hepatic Stellate Cell HSC-T6: A Proteomic Analysis. Drug Design, Development and Therapy, 17, 1819-1829.

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Murine TGF-β1-Induced Cell Responses

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Chemical reagents were purchased from Sigma-Aldrich (St. Louis, MO), and cell culture media was purchased from Invitrogen (Carlsbad, CA), unless otherwise indicated. Carboxyfluorescein succinimidyl ester (CFSE; #C1157) was purchased from Life Technologies (Carlsbad, CA). 3-Methuladenine (3-MA; BML-AP502) was purchased from from Enzo Life Sciences (Farmingdale, NY). and rapamycin (#553210) was purchased from EMD Millipore (Burlington, MA). Recombinant mouse TGF-β1 (#5231) was from Cell Signaling Technology (Danvers, MA). Mouse MCP-1 ELISA kit (#432701) was from Bio Legend (San Diego, CA). Matrigel matrix growth factor reduced (#354230) was from BD Biosciences (San Jose, CA). Lysotracker red DND-99 (L7528; 1:5,000 for staining) was purchased from Invitrogen.

Zhou T., Zheng Y., Sun L., Badea S.R., Jin Y., Liu Y., Rolfe A.J., Sun H., Wang X., Cheng Z., Huang Z., Zhao N., Sun X., Li J., Fan J., Lee C., Megraw T.L., Wu W., Wang G, & Ren Y. (2019). Microvascular endothelial cells engulf myelin debris and promote macrophage recruitment and fibrosis after neural injury. Nature neuroscience, 22(3), 421-435.

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Murine Bone Marrow–derived Mast Cell Generation

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Bone marrow–derived MCs (BMMCs) were generated from bone marrow cells of C57BL/6 mice purchased from Japan SLC (Hamamatsu, Japan) by cultivation in RPMI 1640 (Sigma-Aldrich, St. Louis, MO) containing 10% heat-inactivated FCS (Biowest), 100 U/ml penicillin, 100 μg/ml streptomycin, 50 μM 2-ME, 100 μM MEM nonessential amino acid solution, and 5 ng/ml murine IL-3 (BioLegend) at 37°C for more than 5 wk. FACS was used to confirm that the frequency of BMMCs expressing both FcεRI and c-kit was over 95%. All animal experiments were performed in accordance with the approved guidelines of the Institutional Review Board of Tokyo University of Science, Tokyo, Japan. The Animal Care and Use Committees of Tokyo University of Science specifically approved this study. The human embryonic kidney cell line HEK293T was maintained in DMEM (Sigma-Aldrich) supplemented with 10% FCS. Recombinant mouse TGF-β1 (no. 5231; Cell Signaling Technology) was added to the culture medium at a 1 or 10 ng/ml concentration to stimulate cells.

Kasakura K., Nagata K., Miura R., Iida M., Nakaya H., Okada H., Arai T., Arai T., Kawakami Y., Kawakami T., Yashiro T, & Nishiyama C. (2020). Cooperative Regulation of the Mucosal Mast Cell–Specific Protease Genes Mcpt1 and Mcpt2 by GATA and Smad Transcription Factors. Journal of immunology (Baltimore, Md. : 1950), 204(6), 1641-1649.

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Transforming Growth Factor-β1 Signaling Assay

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Recombinant mouse TGF-β1 (#5231) was purchased from Cell Signaling Technology. Ly364947 (#S2805), GW788388 (#S2750), and S3I-201 (#S1155) were purchased from Selleck Chemicals.

Mirzamohammadi F., Kozlova A., Papaioannou G., Paltrinieri E., Ayturk U.M, & Kobayashi T. (2018). Distinct molecular pathways mediate Mycn and Myc-regulated miR-17-92 microRNA action in Feingold syndrome mouse models. Nature Communications, 9, 1352.

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