Plasma cGMP was determined from samples collected at visit 4 (1996–1998) and stored at −80°C until cGMP measurement was performed in 2017. cGMP concentrations were assessed at the Atherosclerosis Clinical Research Laboratory at Baylor College of Medicine using a competitive ELISA assay (Cayman Chemical Company, MI), with the addition of an optional acetylation procedure per manufacturer protocol. Intra‐ and interassay coefficients of variation were 4.2% and 13.5%, respectively.
Competitive elisa assay
Manufactured by Cayman Chemical
Sourced in United States
The Competitive ELISA assay is a laboratory technique used to detect and quantify the presence of specific analytes in a sample. It utilizes the principle of competitive binding to measure the concentration of the target analyte. The assay involves the use of an immobilized antigen, a labeled antibody, and the sample containing the target analyte. The labeled antibody and the analyte in the sample compete for binding to the immobilized antigen, and the amount of labeled antibody that binds is inversely proportional to the concentration of the target analyte in the sample.
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Lab products found in correlation
5 protocols using competitive elisa assay
1
Plasma cGMP Measurement Protocol
2
Quantifying 2'3'-cGAMP in EV-A71-infected Cells
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EV-A71-infected cells were harvest with pre-cooled PBS and lysed in M-PER™ lysis buffer (78501, Thermo Fisher). 2'3'-cGAMP was measured with a competitive ELISA assay (501700, Cayman Chemical). This assay is based on the competition between native 2'3'-cGAMP in samples and a defined dose of 2'3'-cGAMP-horseradish peroxidase (HRP) conjugate (2'3'-cGAMP-HRP Tracer) for a limited amount of 2'3'-cGAMP Polyclonal Antiserum. The quantification was operated according to the manufacturer instructions.
3
Plasma cGMP Levels Measurement
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Early‐morning fasting blood samples from the baseline exam were stored at −70°C. Plasma cGMP levels were measured from these samples using a competitive ELISA assay (Cayman Chemical, Ann Arbor, MI) at the Atherosclerosis Clinical Research Laboratory at Baylor College of Medicine (Houston, TX). The range of detection was 0.23 to 30 pmol/mL. The intra‐ and interassay coefficients of variation for a control pool with a mean cGMP level of 6.5 pmol/mL were 4.2% and 13.5%, respectively.
4
Quantitative cGMP Measurement via ELISA
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Total levels of cGMP were measured using a competitive ELISA assay (Cayman Chemical) according to the manufacturer's recommendations. A standard curve is generated using samples of known cGMP concentrations provided with the assay and plotted using linear regression to extrapolate samples.
5
cGMP Quantification in HSC-T6 Cells
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The supernatant of HSC-T6 cells were collected and stored at −80°C. Total levels of cGMP were measured using a competitive ELISA assay (Cayman Chemical, USA) according to the manufacturer's recommendations.
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