Mercury 300 mhz instrument

All chemicals purchased from commercial sources were of analytical grade and were used without further purification. The chemicals 3-fluoro-2-hydroxybenzaldehyde and 5-fluoro-2-hydroxybenzaldehyde were prepared by a modified procedure according to Ferguson et al. [43 (link)]. Cell cultures were purchased from the Czech National Collection of Type Cultures. Infrared spectra were recorded using the ATR (attenuated total reflectance) technique on a Nicolet 6700 FT-IR spectrometer from Thermo Scientific (Waltham, MA, USA) in the 600–4000 cm⁻¹ range. Elemental analysis was performed with the help of a Flash2000 instrument from Thermo Scientific. ¹H-, ¹³C- and ¹⁹F-NMR spectra were measured on a Varian Mercury 300 MHz instrument (Palo Alto, CA, USA) using DMSO-d₆ as solvent and TMS (tetramethylsilane) as internal standard. Melting points were determined on a Büchi B-540 apparatus (Büchi Labortechnik AG, Flawil, Switzerland). The conductivities of the copper(II) complexes were evaluated in DMSO solutions (10⁻³ mol/L, 25 °C) on a WTW LF 530 conductometer (Weilheim, Germany).

An oven TCN30 (ArgoLab, Carpi, Modena, Italy) with forced ventilation was used for polyHIPEs polymerization.
UV irradiation of INU-MA HIPE was accomplished with a “Polymer” reactor (HeliosItalquartz, Milan, Italy) provided with a UV lamp of 125 W, with an emission range 250–364 nm. Its emission peak was 310 nm.
Centrifugations were performed with a Beckman Avanti 30 (Beckman, Milano, Italy) equipped with a temperature control.
Lyophilizations were performed by a Christ Alpha 1–4 LSC instrument at −59 °C and 0.016 mbar. ¹H-NMR were acquired with a Varian Mercury 300 MHz instrument (Varian, Segrate, Italy).
SEM images of the surface and the cross-section of the lyophilized polyHIPEs were acquired by a ∑igma Zeissfield emission scanning electron microscope (FE-SEM) (Zeiss, Milan, Italy). Prior to the analysis, the samples were stuck on stubs with a carbon adhesive disc and subsequently subjected to coating by a 2 nm layer of palladium by using an electron beam evaporator. The SEM probing e-beam was set at an acceleration voltage of 3 kV; 30μm slit aperture and the in-lens detector were used.

The photochemical reactions were performed in a 400 W medium pressure mercury-lamp in a 2 L quartz immersion well reactor (reaction volume 1.2 L) fitted with a no. 3408 Pyrex glass filter sleeve supplied by Photochemical Reactors Ltd. Silica gel 60A C.C. 40–43 μm from SDS were used for flash chromatography. Melting points were obtained in sealed capillary tubes on a Stuart Scientific melting point apparatus SMP3. NMR-spectra were measured on a Varian Mercury 300 MHz instrument with tetramethylsilane or solvent peak as internal reference (CDCl₃: 0.0 ppm, 77.0 ppm; CD₃OD: 3.31 ppm, 49.0 ppm). HRMS analyses were performed on an JMS T100 GC-AccuTOF^TM EI-TOF from Jeol.