Cells activated in an MLR were allowed to incubate for up to 3 days before harvesting. [3H]Thymidine (0.5 μCi/well; MPBiomedicals) was added 24 h before harvesting (SkatronInstruments) using Type A filter mats (Perkin-Elmer Life and Analytical Sciences) and a beta plate scintillation mixture (Perkin-Elmer). Disintegrations per minute were determined using a liquid scintillation counter (1205 Betaplate; Perkin-Elmer).
Type a filter mats
Manufactured by PerkinElmer
Type A filter mats are a component used in laboratory equipment. They serve the core function of filtration, designed to remove particulates and contaminants from liquid or gas samples during analytical testing or sample preparation processes.
Automatically generated - may contain errors
2 protocols using type a filter mats
1
Measuring Cell Proliferation in MLR
2
Leukocyte Activation and Proliferation Assay
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Leukocyte activation was performed using 96-well flat-bottom culture plates (Greiner Bio-One; Germany) for 72 h at 37 °C in the presence of 5% CO2. For standard two-way MLR assays, responder cells (total splenocytes, 2 × 105) were co-cultured with an equal number of stimulator splenocytes in 200 μl medium14 (link),16 (link). Thymidine (1 μ Ci/well; PerkinElmer Life and Analytical Sciences) was added 18–24 h before recovering (Inotech Biosystems International Inc.) using Type A filter mats (PerkinElmer Life and Analytical Sciences) and a beta-plate scintillation mixture (PerkinElmer). CPM were determined using a liquid scintillation analyzer (Packard 1900CA, Packard Instrument Co.). Data were expressed as the mean CPM of triplicate determination, and converted into proliferation percentages. 100% proliferation refers to vehicle treated allogeneic group. Splenocytes background readout values (medium alone) were deducted from the results.
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