Cd4 sc 1140

Immunohistochemistry and immunofluorescence analyses were performed as previously described^{42 (link)}. Uterine sections from paraffin-embedded tissues were preincubated with 10% normal serum in phosphate-buffered saline (PBS) and incubated with anti-TGF-β2 (ab36495; Abcam, Cambridge, MA, USA), anti-E-cadherin (610181; BD Bioscience, San Jose, CA, USA), anti-F4/80 (nbp2-12506; Novus, Littleton, CO, USA), anti-CD68 (SC-5474; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-CD4 (SC-1140; Santa Cruz Biotechnology, Santa Cruz, CA, USA), and anti-vimentin (ab92547; Abcam, Cambridge, MA, USA and SC-6260; Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies in 10% normal serum in PBS. On the following day, sections were washed in PBS and incubated with a secondary antibody (Vector Laboratories, Burlingame, CA, USA) for 1 h at room temperature. Immunoreactivity was detected using the Vectastain Elite DAB Kit (Vector Laboratories). Images were captured with a confocal microscope (510 NLO confocal microscope; Carl Zeiss, Thornwood, NY, USA).