Dry tetrahydrofuran

Manufactured by Merck Group

Dry tetrahydrofuran is a laboratory solvent used to dissolve and dilute various organic compounds. It is a clear, colorless liquid with a distinctive odor. The product is anhydrous, meaning it contains minimal water content.

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Lab products found in correlation

Milli q integral 3 system, by Merck Group (3 mentions) Triethylamine, by Merck Group (2 mentions) Acetone, by Merck Group (2 mentions) Triethanolamine, by Merck Group (1 mentions) Meo2ma, by Merck Group (1 mentions) Milli q water, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) 1 bromodecane, by Merck Group (1 mentions) Ethanol, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Carbonyldiimidazole, by Merck Group (1 mentions)

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Tetrahydrofuran (624 citations)

4 protocols using dry tetrahydrofuran

Silane-Mediated Polymer Brush Synthesis

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Unless stated otherwise, all chemical reagents
were utilized without undergoing additional purification. (3-Aminopropyl)triethoxysilane
(APTES), 4-cyano-4-(phenylcarbonothioylthio)pentanoic acid N-succinimidyl ester (RAFT-NHS), acetone (99.5%), di(ethylene
glycol) methyl ether methacrylate (MeO2MA), eosin Y (EY), ethanol
(EtOH) (99.9%), dry tetrahydrofuran (99.9%), triethanolamine (TEOA),
and triethylamine were acquired from Sigma-Aldrich (Merck). N-(2-Hydroxypropyl) methacrylamide (HPMA) was obtained from
Poly Sciences, Inc. Quartz crystal microbalance chips were acquired
Quantum Design GmbH. Glass substrates were acquired from Micronit.
Silicon substrates were purchased from Siltronix. Milli-Q water was
generated using a Milli-Q integral 3 system from Millipore in Molsheim,
France.

Kuzmyn A.R., Ypma T.G, & Zuilhof H. (2024). Tunable Cell-Adhesive Surfaces by Surface-Initiated Photoinduced Electron-Transfer-Reversible Addition–Fragmentation Chain-Transfer Polymerization. Langmuir, 40(7), 3354-3359.

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Synthesis and Characterization of Zwitterionic Polymer Coatings

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All chemical reagents were used without further
purification unless otherwise specified. 4-Cyano-4-(phenylcarbonothioylthio)pentanoic
acid N-succinimidyl ester (RAFT-NHS), N-[3-(dimethylamino)propyl]methacrylamide, 1-bromodecane, (3-aminopropyl)triethoxysilane
(APTES), triethanolamine (TEOA), eosin Y (EY), triethylamine (TEA),
ethanol (EtOH, 99.9%), acetone (99.5%), dry tetrahydrofuran (THF,
99.9%), dry diethyl ether (99.9%), and acetonitrile (99.9%) were purchased
from Sigma-Aldrich. Carboxybetaine methacrylamide (CBMA) was synthesized according to a previously described procedure.^{31 (link),52 (link),53 (link)} Silicon substrates were acquired
from Siltronix. Deionized water was produced with a Milli-Q integral
3 system (Millipore, Molsheim, France (Milli-Q water)).

Kuzmyn A.R., Teunissen L.W., Kroese M.V., Kant J., Venema S, & Zuilhof H. (2022). Antiviral Polymer Brushes by Visible-Light-Induced, Oxygen-Tolerant Covalent Surface Coating. ACS Omega, 7(43), 38371-38379.

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Protein-Resistant Polymer Coatings

Cited 1 time

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All
chemical reagents were used without further
purification, unless otherwise specified. 4-Cyano-4-(phenylcarbonothioylthio)pentanoic
acid N-succinimidyl ester (RAFT-NHS), (3-aminopropyl)triethoxysilane
(APTES), TEOA, EY, triethylamine (TEA), oligo(ethylene glycol) methyl
ether methacrylate (MeOEGMA, average M_n 300), ethanol (EtOH, 99.9%), acetone (99.5%), dry tetrahydrofuran
(THF, 99.9%), and phosphate-buffered saline (PBS) were purchased from
Sigma-Aldrich; HPMA was obtained from Polysciences, Inc.; and streptavidin-Alexa488
conjugate (Str-Alexa488) and BS albumin-Alexa488 conjugate (BSA-Alexa488)
were purchased from Fisher Thermo Scientific. Silicon substrates were
acquired from Siltronix. Deionized water was produced using a Milli-Q
integral 3 system (Millipore, Molscheim, France). (3-Acryloylamino-propyl)-(2-carboxy-ethyl)-dimethyl-ammonium
(CBMA) was synthesized according to a previously described procedure.^{11 (link),12 (link),20 (link)} BS was obtained and biotinylated
as previously described.^{21 (link)}

Kuzmyn A.R., Nguyen A.T., Teunissen L.W., Zuilhof H, & Baggerman J. (2020). Antifouling Polymer Brushes via Oxygen-Tolerant Surface-Initiated PET-RAFT. Langmuir, 36(16), 4439-4446.

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Functionalization of pCA and 3-Br-pCA

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To facilitate incorporation into the PYP protein, pCA and 3-Br-pCA must be functionalized into an acid anhydride. The functionalization should be performed the same day as chromophore incorporation to minimize hydrolysis. 82 mg pCA (Sigma-Aldrich) or 122 mg 3-Br-pCA (0.5 mmol) was added to a dry round-bottom flask with a stir bar along with 91 mg (0.56 mmol) carbonyldiimidazole (Sigma-Aldrich). The flask was flushed with argon before addition of 12 mL dry tetrahydrofuran (Sigma-Aldrich). The mixture was stirred for 1 hour at room temperature under argon, then the solvent was removed with rotary evaporation to yield a yellow solid. The flask with the functionalized chromophore was then used during the protein purification process as described below.

Romei M.G., Lin C.Y, & Boxer S.G. (2020). Structural and spectroscopic characterization of photoactive yellow protein and photoswitchable fluorescent protein constructs containing heavy atoms. Journal of photochemistry and photobiology. A, Chemistry, 401, 112738.

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