Endothelial cell medium (ECM) and pericyte medium (PM) were products of ScienCell Research Laboratories (San Diego, CA, USA). Antibodies against Ki67 (#12,202), α-SMA (#19,245), bFGF (#20,102), FGFR1 (#76,123), FGFR2 (#23,328), β-actin (#23,328) and HRP-conjugated anti-rabbit IgG (#7074) were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibodies against Gli1 (AF3455) and CD31 (AF3628), and a Proteome Profiler Human Angiogenesis Array Kit (ARY007) were obtained from R&D Systems (Minneapolis, MN, USA). Alexa Fluor 594-conjugated donkey anti-rabbit IgG (ab150064) and Alexa Fluor488- conjugated donkey anti-mouse IgG (ab150073) were obtained from Abcam (Cambridge, UK). GANT-61, cycloheximide (CHX) and MG132 were purchased from TargetMol (Shanhai, China). Matrigel was obtained from BD Biosciences (Franklin Lakes, NJ). Recombinant human bFGF was purchased from BioVision (Palo Alto, CA, USA). A ChIP PCR kit was obtained from Merck Millipore (Billerica, MA, USA). A dual luciferase reporter gene assay kit was provided by Promega (Madison, WI, USA). Rhodamine-labeled lysinated dextran (70-kDa) was product from Thermo Fisher Scientific (Waltham, MA, USA). PKH 26, PKH 67 and other reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA).
Chip pcr kit
Manufactured by Merck Group
Sourced in United States
The ChIP PCR kit is a laboratory equipment product designed for chromatin immunoprecipitation (ChIP) and subsequent polymerase chain reaction (PCR) analysis. The kit provides the necessary reagents and protocols to perform ChIP experiments and analyze the precipitated DNA fragments using PCR techniques.
Automatically generated - may contain errors
Lab products found in correlation
Dual luciferase reporter gene assay kit, by Promega (2 mentions)
Alexa fluor 594 conjugated donkey anti rabbit igg ab150064, by Abcam (2 mentions)
Matrigel, by BD (2 mentions)
Mg132, by Targetmol (1 mentions)
Pericyte medium pm, by ScienCell (1 mentions)
Hrp conjugated anti rabbit igg 7074, by Cell Signaling Technology (1 mentions)
Cycloheximide (chx), by Targetmol (1 mentions)
Pkh26, by Merck Group (1 mentions)
Pkh67, by Merck Group (1 mentions)
Endothelial cell medium (ecm), by ScienCell (1 mentions)
Hrp conjugated anti rabbit igg antibody, by Cell Signaling Technology (1 mentions)
Emt antibody sampler kit, by Cell Signaling Technology (1 mentions)
D luciferin, by Yeasen (1 mentions)
Phospho gsk 3β ser9 antibody, by Cell Signaling Technology (1 mentions)
Gsk3β antibody, by Cell Signaling Technology (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions)
N cadherin, by Cell Signaling Technology (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
2 protocols using chip pcr kit
1
Angiogenesis Regulation in Endothelial Cells
2
Epithelial-Mesenchymal Transition Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Gli1 antibody (AF3455) was purchased from R&D Systems Inc (Minneapolis, MN, USA). EMT antibody sampler kit (#9782) (including N-cadherin, Vimentin, Snail, E-cadherin, and ZO-1), HRP-conjugated anti-rabbit IgG antibody (#7074), glycogen synthase kinase 3β (GSK-3β) antibody (#12456), and phospho-GSK-3β (Ser9) antibody (#9336) were acquired from cell signaling technology (Danvers, MA, USA). Alexa Fluor 594-conjugated donkey anti-rabbit IgG (ab150064) and Alexa Fluor 488 donkey anti-rabbit IgG (ab150073) were obtained from Abcam (Cambridge, UK). Lipofectamine 3000 and Opti-MEM were products of Invitrogen (Thermo Fisher Scientific, Waltham, MA, USA). ChIP PCR kit was a product of Merck Millipore (Billerica, MA, USA). Dual luciferase reporter gene assay kit was obtained from Promega (Madison, WI, USA). Wide type and mutational Snail vector and siRNA were purchased from Qingke biotech (Wuhan, China). GANT-61, cycloheximide (CHX), MG132 and LiCl were obtained from Shanghai Pottery Biotechnology (Shanghai, China) and dissolved in dimethyl sulfoxide. Matrigel was obtained from BD Biosciences (Franklin Lakes, NJ, USA). d -Luciferin was obtained from Yeasen (Shanghai, China). Other agents were purchased from Sigma–Aldrich (St Louis, MO, USA).
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