Lymphocyte subsets were assayed by using a lyse/no-wash procedure based on a single-platform technique of ten-color flow cytometry (BD FACS Canto II : U6573380-00541, USA). The reagents were BD Multitest IMK kit (catalog no.: 662965) containing BD Multitest CD3FITC/CD8PE/CD45PerCP/CD4APC and BD Multitest CD3 FITC/CD16+CD56+PE/CD45PerCP/CD19APC, and BD Multitest IMK kit lysing solution (catalog no.: 91–1087). The EDTA blood collecting tubes and trucount tubes (catalog no.: 340334) were also purchased from BD Biosciences, USA.
Multitest imk kit lysing solution
Manufactured by BD
The BD Multitest IMK kit lysing solution is a reagent used in the preparation of samples for flow cytometry analysis. It is designed to lyse red blood cells and preserve the integrity of white blood cells, enabling the detection and enumeration of specific cell populations.
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Lab products found in correlation
Multitest imk kit, by BD (1 mentions)
Edta blood collecting tubes, by BD (1 mentions)
Multitest cd3fitc cd8pe cd45percp cd4apc, by BD (1 mentions)
Multitest cd3 fitc cd16 cd56 pe cd45percp cd19apc, by BD (1 mentions)
Trucount tube, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Multitest cd3 cd16 cd56 cd45 cd19 reagent, by BD (1 mentions)
2 protocols using multitest imk kit lysing solution
1
Flow Cytometry Analysis of Lymphocyte Subsets
2
Whole Blood Immunophenotyping by Flow Cytometry
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Total 227 cases collected between July 2016 and September 2019 and performed by flow cytometry. The manipulation was referred to BD operating instruction.
For each sample, two trucount tubes labeled with the simple identification number such as letters A and B to distinguish from each other.
20 μL of BD Multitest CD3/CD4/CD8/CD45 reagent was pipetted into the bottom of each tube marked A.
20 μL of BD Multitest CD3/CD16 + CD56/CD45/CD19 reagent were pipetted into the bottom of each tube marked B.
50 μL of well-mixed and anticoagulated whole blood was pipetted into the bottom of every tube using the method of reverse pipetting.
Then vortexed gently to mix, incubate for 15 min in dark at room temperature.
Finally, 450 μL of 1× BD Multitest IMK kit lysing solution was pipetted into every tube (Dilute the 10× concentrate 1:10 with room temperature deionized water). The solution was vortexed gently to mix and incubated for 15 min in dark at room temperature.
Samples were analyzed on the flow cytometer.
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