Multiplex ELISA antibody array was performed using rat cytokine array Q67 kit (QAR-CAA-67, RayBiotech, Norcross GA) per manufacture service. rat cytokine array Q67 kit allows simultaneous quantification of multiple (67) rat cytokines (https://www.raybiotech.com/high-density-quan-titative-biomarker-array-services.html#100-rat-biomarkers ) in CSF samples, including cytokines, inflammatory factors, growth factors, and receptors (referred to collectively as soluble factors in this report). Each sample was tested individually in quintuplicate, and the measurements were averaged. The actual cytokine concentration was directly quantified according to a standard curve performed in parallel for each cytokine as part of the array.
Rat cytokine array q67 kit
Manufactured by RayBiotech
The Rat Cytokine Array Q67 Kit is a multiplex assay used to simultaneously detect the relative expression levels of 67 different rat cytokines and chemokines in a single sample. The kit utilizes a membrane-based antibody array format to provide a quantitative analysis of multiple analytes. It is designed to facilitate the screening of complex biological samples for the presence of various rat cytokines and related proteins.
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2 protocols using rat cytokine array q67 kit
1
Multiplex ELISA Cytokine Profiling
2
Multiplex Cytokine Profiling in Rat Heart
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Multiple ELISA antibody array detection was performed using a Rat Cytokine Array Q67 Kit (QAR-CAA-67; RayBiotech, Norcross GA) according to the manufacturer’s protocol. The Rat Cytokine Array Q67 Kit was used to simultaneously and quantitatively assay multiple (67) rat factors, including chemokines, inflammatory cytokines, growth factors, and receptors (commonly referred to in this report as soluble factors), in samples. We used rat right ventricular tissue homogenates to perform the assay. Each sample was tested individually in quintuplicate, and the values were averaged. The actual concentration of each cytokine was directly quantified according to a standard curve in parallel as part of the array.
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