Pcdna empty vector vector

siRNAs against XIST (si-XIST-1, si-XIST-2), siRNA control (si-con), pcDNA-XIST, pcDNA empty vector (vector), miR-29c mimic (miR-29c), miRNA control (miR-con), miR-29c inhibitor (anti-miR-29c), and control inhibitor (anti-miR-con) were purchased from GenePharma Co., Ltd. (Shanghai, China). NPC cells were seeded into 6-well plates and cultured with complete medium without antibiotics at least 24 h prior to transfection. Then, cells were transiently transfected with siRNAs, miRNAs, or pcDNAs, or cotransfected with si-XIST and anti-miR-29c or anti-miR-con using Lipofectamine™ 2000 (Invitrogen). The cells were harvested at different time points for analysis post-transfection.

miR-218 mimics (miR-218-5p), miR-218 inhibitor, control miRNA (miR-control), pcDNA-Runx2, pcDNA empty vector (vector), siRNA against Runx2 (si-Runx2), and siRNA control (si-control) were all obtained from GenePharma Co., Ltd. (Shanghai, China). TPC-1 and K-1 cells at approximately 80% confluence were transfected with mimics, siRNAs, or plasmids using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA). At 48 hours post-transfection, cells were harvested and subjected to next analyses.