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Alexa fluor 555 and alexa 488 donkey anti mouse and rabbit conjugated antibodies

Manufactured by Thermo Fisher Scientific
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Alexa Fluor 555 and Alexa 488 donkey anti-mouse and rabbit-conjugated antibodies are fluorescent secondary antibodies used for detection and visualization in immunoassays and microscopy applications. These antibodies are conjugated to the Alexa Fluor 555 and Alexa 488 dyes, which provide bright and photostable fluorescent signals. They are designed to bind to primary antibodies raised in mouse or rabbit, allowing for the indirect labeling of target proteins or cellular structures.

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Lab products found in correlation

Lsm 780, by Zeiss (4 mentions) Ab186734, by Abcam (2 mentions) Poly lysine 0.01, by Merck Group (2 mentions) Biotinylated 8 ohdg antibody, by StressMarq Biosciences (2 mentions) Epr15581, by Abcam (2 mentions)

Close spelling variants of this product

Alexa Fluor 488-conjugated anti-mouse and Alexa Fluor 555-conjugated anti-rabbit antibodies Donkey anti-rabbit and anti-mouse Alexa Fluor 488 antibodies Alexa Fluor 488- and 555-conjugated antibodies Alexa Fluor 555-conjugated donkey anti-rabbit Alexa Fluor 555-conjugated donkey anti-mouse Alexa Fluor 488-conjugated donkey anti-rabbit Alexa Fluor 488-conjugated donkey anti-mouse Alexa Fluor 555 donkey anti-rabbit Alexa Fluor 488-conjugated anti-mouse/rabbit Donkey anti-mouse Alexa Fluor 555

2 protocols using alexa fluor 555 and alexa 488 donkey anti mouse and rabbit conjugated antibodies

1

Neutrophil Oxidative DNA Damage Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neutrophils seeded over coverslip chambers (LabTek) pre-coated with poly-lysine 0.01% (Sigma) were fixed in 4% paraformaldehyde overnight and permeabilized in 0.2% Triton for 10 minutes. Oxidized DNA was detected by biotinylated 8-OHdG antibody (StressMarq Biosciences) used at 1 µg/mL and mitochondria (TOM20) was detected using rabbit-anti-human mAb EPR15581 (1:250, ab186734, Abcam), followed by incubation with Alexa Fluor 555 and Alexa 488 donkey anti-mouse and rabbit-conjugated antibodies at 1:250 (Invitrogen) and Hoechst 1:1000. Cells were resuspended in Prolong and visualized with a Zeiss LSM780 laser confocal fluorescence microscope. Images were acquired using the microscope’s associated software.
Lood C., Blanco L.P., Purmalek M.M., Carmona-Rivera C., De Ravin S.S., Smith C.K., Malech H.L., Ledbetter J.A., Elkon K.B, & Kaplan M.J. (2016). Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nature medicine, 22(2), 146-153.
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2

Neutrophil Oxidative DNA Damage Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neutrophils seeded over coverslip chambers (LabTek) pre-coated with poly-lysine 0.01% (Sigma) were fixed in 4% paraformaldehyde overnight and permeabilized in 0.2% Triton for 10 minutes. Oxidized DNA was detected by biotinylated 8-OHdG antibody (StressMarq Biosciences) used at 1 µg/mL and mitochondria (TOM20) was detected using rabbit-anti-human mAb EPR15581 (1:250, ab186734, Abcam), followed by incubation with Alexa Fluor 555 and Alexa 488 donkey anti-mouse and rabbit-conjugated antibodies at 1:250 (Invitrogen) and Hoechst 1:1000. Cells were resuspended in Prolong and visualized with a Zeiss LSM780 laser confocal fluorescence microscope. Images were acquired using the microscope’s associated software.
Lood C., Blanco L.P., Purmalek M.M., Carmona-Rivera C., De Ravin S.S., Smith C.K., Malech H.L., Ledbetter J.A., Elkon K.B, & Kaplan M.J. (2016). Neutrophil extracellular traps enriched in oxidized mitochondrial DNA are interferogenic and contribute to lupus-like disease. Nature medicine, 22(2), 146-153.
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