Mouse monoclonal antibodies against Flag (Origene, 1:2000, F3165), HA (Origene, 1:2000, H6908), β-actin (Sigma, 1:10,000, A2228), Myc (CST, 1:1000, 5605), p-IκBα (CST, 1:1000, 9246 L); rabbit antibodies against p-IRF3 (CST, 1:500, 37829), USP19 (Abcam, 1:1000, ab93159), TRIF (Abcam, 1:1000, ab180689), p65 (Santa Cruz Biotechnology, 1:1000, 71675), p-p65(S536) (CST, 1:1000, 3033),TBK1 (Abcam, 1:1000, ab40676) and p-TBK1 (Abcam, 1:1000, ab109272), ubiquitin (Abcam, 1:500, ab134953), K27-linkage specific polyubiquitin (Abcam, 1:1000, 181537), TLR3 (CST, 1:500, 6961), TLR4 (R&D, 1:500, AF1478), TRAM (Abcam, 1:500, ab96106), KCTD10 (Proteintech, 1:1000, 27279–1-AP); poly(I:C) (Invivogen), LPS (Sigma), R848 (Invivogen), PGN (Invivogen), human IFN-γ (Peprotech), murine M-CSF (Peprotech), Trizol (Takara Bio), SYBR Green (BIO-RAD), dual-specific luciferase assay kit (Promega, E1980), polybrene (Millipore,TR-1003-G), type II collagenase (Worthington), DNase I (Sigma-Aldrich), and d -galactosamine hydrochloride (D-Gal) (Sigma); and ELISA kits for TNF (Biolegend), IL-6 (Biolegend), CXCL10 (Boster) and IFN-β (PBL) were purchased from the indicated companies. HEK293 cells were obtained from ATCC. SeV (Cantell strain) (Charles River Laboratories), HSV-1 (KOS strain) (China Center for Type Culture Collection, Wuhan, China) were obtained from the indicated companies.
Ab93159
Manufactured by Abcam
Sourced in United Kingdom
Ab93159 is a primary antibody designed for use in immunohistochemistry applications. The product details and specifications are available on the Abcam website.
Automatically generated - may contain errors
Lab products found in correlation
Sybr green, by Bio-Rad (1 mentions)
Trizol, by Takara Bio (1 mentions)
Dnase 1, by Merck Group (1 mentions)
Human ifn γ, by Thermo Fisher Scientific (1 mentions)
Collagenase type 2, by Worthington (1 mentions)
Hek293 cell, by American Type Culture Collection (1 mentions)
Dual specific luciferase assay kit, by Promega (1 mentions)
Polybrene, by Merck Group (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Murine m csf, by Thermo Fisher Scientific (1 mentions)
H6908, by Merck Group (1 mentions)
Ab109272, by Abcam (1 mentions)
Ab134953, by Abcam (1 mentions)
Ab40676, by Abcam (1 mentions)
Ifn β, by PBL Assay Science (1 mentions)
Cxcl10, by Boster Bio (1 mentions)
F3165, by OriGene (1 mentions)
Elisa kits for tnf, by BioLegend (1 mentions)
Interleukin 6 (il 6), by BioLegend (1 mentions)
Anti flag, by Merck Group (1 mentions)
3 protocols using ab93159
1
Antibody Characterization and Signaling Pathway Analysis
2
Immunoblotting Assay with Antibodies
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The following reagents were used: DMSO, doxycycline (both Sigma Aldrich), G418 (Promega, Duebendorf, Switzerland), puromycin (ThermoFisher Scientific AG). The following commercial antibodies were used: anti-Flag (dilution 1:1000, clone M2, Sigma Aldrich), anti-FLI1 (1:1000, MBS300723 MyBioSource LLC, San Diego, CA, USA), anti-GAPDH (1:1000, D16H11 Cell Signaling Technology, Beverly, MA, USA), anti-HA (both 1:1000, clone 6E2 Cell Signaling Technology and 05–904 Millipore), anti-Myc (1:1000, clone 9B11 Cell Signaling Technology), anti-p27 (1:200, clone DCS-72.F6 ThermoFisher Scientific AG), anti-Tubulin (1:1000, clone DM1A Sigma Aldrich), and anti-USP19 (1:1000 (WB) A301-587A Bethyl Laboratories, Montgomery, TX, USA and 1:200 (IHC) or 1:1000 (WB) ab93159 Abcam, Cambridge, UK).
3
Western Blot Analysis of Antioxidant and Inflammatory Markers
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Protein lysates were prepared in RIPA lysis buffer (Pierce, Rockford, IL, USA) and quantified using Bradford reagent (Pierce). Proteins were separated by gel electrophoresis and transferred onto NC membrane. After blocking, the blots were incubated with primary antibody at 4 °C overnight, followed by the incubation with corresponding secondary antibody. Signals were detected using a ECL kit (Pierce). Antibodies used in Western blot: anti-SOD (1:5000, ab13498), anti-GPx (1:1000, ab22604), anti-USP19 (1:1000, ab93159), anti-FOXO1 (1:1000, ab52857), anti-IL-10 (1:1000, ab33471), anti-IL-10R1 (1:1000, ab89883), anti-MMP9 (1:1000, ab38898) and anti-β-actin antibodies (1:2000, ab8226, Abcam).
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