Gcms tq8040

Metabolites in the cecal contents were analyzed by GC/MS-TQ8040 (Shimazu, Kyoto, Japan) with a BPX-5 column (30 m × 0.25 mm i.d.; film thickness 1.00 μm, Trajan Scientific and Medical, Vic., Australia) for short chain fatty acids (SCFAs), and a DB-5 column (30 m × 0.25 mm i.d.; film thickness 1.00 μm, J&W Scientific Inc, Folsom, CA, USA) for other previous described metabolites [12 (link)]. Mass spectrum peaks were detected using GCMSsolution software (Shimazu), and the retention time correction of peaks was performed based on the retention time of a standard alkane series mixture (C9 to C33). Metabolites were detected by Smart Metabolites Database (Shimazu) which registered 12 spectrums for BPX-5 column and 475 spectrums for DB-5 column.

Pesticide concentrations were determined using tandem mass spectrometry (GCMS-TQ 8040, Shimazu Corp., Japan). GC separation was performed using an Agilent HP-5 MS (30 m × 0.25 mm × 0.25 μm) capillary column. The following conditions were set for the oven: 50 °C, maintain for 1 min; 50–150 °C at intervals of 25 °C/min, hold for 1 min; 150–300 °C at intervals of 10 °C/min, hold for 5 min. The temperature at the inlet was set to 250 °C. With a 2.0 mL/min flow rate, 99.999% pure helium was used as the carrier gas for chromatographic analysis. An injection volume of 2 μL was analyzed in the splitless mode under high pressure conditions (200 kPa).
A triple quadrupole mass spectrometer in electron impact (EI) ionization mode was operated with a 70 eV ionization voltage and 60 µA of emission current. The interface (transfer line to the tandem MS), ion source, and quadrupole temperatures were maintained at 230 °C and 150 °C. Multiple reaction monitoring (MRM) mode was used for target detection.

Composition analysis of volatile oil was performed by gas chromatography-mass spectrometry (GC-MS) using Shimadzu GCMS-TQ8040 (Shimadzu Corporation, Japan) on a Rxi-5MS capillary column (30 m × 0.25 mm id, film thickness 0.25 μm; Shimadzu, Japan). The carrier gas was helium (flow rate of 1.36 ml/min). GC-MS was carried out using the following temperature program: initial temperature was set at 180 °C and held for 3 min, followed by 6 °C/min ramp to 240 °C and hold for 3 min, followed by 3 °C/min ramp to 275 °C and hold for 3 min, followed by 5 °C/min ramp to 300 °C and hold for 3 min, followed by 5 °C/min ramp to a final temperature of 330 °C and hold for 5 min. The injection temperature was set at 290 °C and the injection volume was 1 μl (splitless mode). Detector parameters used for GC-MS analyses were as follows: interface temperature, 320 °C; ion source temperature, 300 °C; mass spectrometry was performed using Q3 scan with an m/z 45-500 scanning range. Chromatograms and mass spectra were evaluated using the GCMS solution software (Shimadzu Corporation, Japan).