Most drugs were dissolved in water to make stock solutions (typically 10,000x concentrations) that were frozen as aliquots and used as needed during experiments. Acetylcholine (Fisher Scientific) was bath-applied with physostigmine hemisulfate (eserine; Tocris Bioscience), a blocker of Acetylcholinesterase. Atropine and pirenzepine dihydrochloride were purchased from Sigma-Aldrich. Tetrodotoxin (TTX) citrate and CGP 52432 were purchased from HelloBio, Inc. Clozapine N-oxide (CNO) dihydrochloride was purchased from Tocris Biosciences. SR-95531 (gabazine) was purchased from MedChemExpress and dissolved in DMSO for stock solutions.
Pirenzepine dihydrochloride
Manufactured by Merck Group
Sourced in Canada, United States
Pirenzepine dihydrochloride is a chemical compound that acts as a muscarinic acetylcholine receptor antagonist. It is used as a research tool in the study of muscarinic receptor subtypes.
Automatically generated - may contain errors
Lab products found in correlation
Physostigmine hemisulfate eserine, by Bio-Techne (2 mentions)
Acetylcholine, by Thermo Fisher Scientific (1 mentions)
Clozapine n oxide dihydrochloride, by Bio-Techne (1 mentions)
Uv star 96 well microplate, by Greiner (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Atropine sulphate, by Merck Group (1 mentions)
Atropine sulfate, by Merck Group (1 mentions)
Pd 102807, by Bio-Techne (1 mentions)
Cno dihydrochloride, by Bio-Techne (1 mentions)
D ap5, by Bio-Techne (1 mentions)
4 protocols using pirenzepine dihydrochloride
1
Pharmacological Modulation of Neurotransmission
2
Atropine and Pirenzepine Assay Protocol
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Atropine sulphate, pirenzepine dihydrochloride and 10× phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich Co. (Oakville, ON, Canada). UV-Star 96-well microplates, from Greiner Bio-One, and lens paper were purchased from VWR (Mississauga, ON, Canada). All reagents and materials were used as received.
3
Radioligand Binding Assay for Muscarinic Receptors
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Atropine sulfate and pirenzepine dihydrochloride were purchased from Sigma-Aldrich (Sigma-Aldrich Co, St. Louis, MO, USA). PD102807 was purchased from Tocris Bioscience (Tocris Bioscience, Bristol, United Kingdom). MT3 toxin and MT7 toxin were purchased from Peptide Institute (Peptide Institute, Inc., Osaka, Japan). Pirenzepine [N-methyl-3H] (83.4 Ci/mmol), and AFDX-384 [2,3-dipropylamino-3H] (100.0 Ci/mmol) were from American Radiolabeled Chemicals (ARC, Inc.), Qinuclidinyl benzilate L-[benzilic-4,4′-3H] (50.5 Ci/mmol) was purchased from Perkin Elmer (Perkin Elmer Inc., USA).
4
Pharmacological Reagents for Neuroscience
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Most drugs were dissolved in water to make stock solutions (typically at 10,000× concentration) that were frozen as aliquots and used as needed during experiments. ACh (Fisher Scientific) was bath-applied with physostigmine hemisulfate (eserine; Tocris Bioscience), a blocker of acetylcholinesterase (AChE). Atropine and pirenzepine dihydrochloride were purchased from Sigma-Aldrich. TTX citrate and CGP 52432 were purchased from HelloBio, Inc. CNO dihydrochloride, DNQX, and D-AP5 were purchased from Tocris Bioscience. SR-95531 (gabazine) was purchased from MedChemExpress and dissolved in DMSO for stock solutions.
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