PVF and PVP were evaluated at the time of sacrifice immediately before collecting blood and tissue samples, as previously proposed by Yagi et al. [32 (link)]. PVF was therefore measured with a transit-time perivascular flowmeter (T403, Transonic Systems Inc., Ithaca (NY), USA) utilizing a transonic flow probe (MA2PSB, Transonic Systems Inc., Ithaca (NY), USA) and PVP was measured via direct puncture of the anterior wall of the portal vein with a 27-gauge needle (BD Microlance 3, Becton Dickinson GmbH, Heidelberg, Germany) and subsequent recording with a corresponding monitoring device (Sirecust 404, Siemens, Erlangen, Germany). PVF and PVP were investigated to assess gross vascular perfusion.
Microlance 3
Manufactured by BD
Sourced in Germany, United States, Ireland, Spain
The BD Microlance 3 is a sterile, single-use hypodermic needle designed for medical and laboratory use. It features a sharp, beveled tip for precise penetration and a durable, flexible stainless steel construction. The needle is available in a range of gauges and lengths to accommodate various applications.
Automatically generated - may contain errors
Lab products found in correlation
Sirecust 404, by Siemens (2 mentions)
Sodium chloride (nacl), by B. Braun (2 mentions)
Xylavet, by CP-Pharma (2 mentions)
Ketanest s, by Pfizer (2 mentions)
Ma2psb, by Transonic Systems (1 mentions)
Ma2psb flow probe, by Transonic Systems (1 mentions)
Female c57bl 6 mice, by Janvier Labs (1 mentions)
24 well plate, by Sarstedt (1 mentions)
Blood cell culture dna mini kit, by Qiagen (1 mentions)
Plastipak, by BD (1 mentions)
Glycerol, by Avantor (1 mentions)
Tween 80, by Avantor (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Nuclepore track etch membrane, by Cytiva (1 mentions)
Polystat 36, by Thermo Fisher Scientific (1 mentions)
Pd10 sephadex g 25m column, by GE Healthcare (1 mentions)
5 ml syringe, by BD (1 mentions)
Whatman, by Cytiva (1 mentions)
Vacutainer plasma tube, by BD (1 mentions)
Axio imager z1 microscope, by Zeiss (1 mentions)
32 protocols using microlance 3
1
Evaluating Portal Vascular Function
2
Liver Microcirculation and Portal Flow
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Graft microcirculation and red blood cell velocity were measured using an O2C device with a corresponding surface probe (O2C-oxygen to see device, LF1 surface probe; LEA Medizintechnik GmbH, Giessen, Germany). Mean of the measurements from 4 standard points on the liver surface were used to characterize graft microcirculation.
Transit-time perivascular flowmeter was used for portal venous flow measurements (T403 device, MA2PSB flow probe; Transonic Systems, Inc., Ithaca, NY, USA). Portal venous pressure was measured using a monitoring device (Sirecust 404; Siemens, Erlangen, Germany) following direct puncture of the portal vein with a 27-gauge needle (BD Microlance 3; Becton Dickinson GmbH, Heidelberg, Germany).
Transit-time perivascular flowmeter was used for portal venous flow measurements (T403 device, MA2PSB flow probe; Transonic Systems, Inc., Ithaca, NY, USA). Portal venous pressure was measured using a monitoring device (Sirecust 404; Siemens, Erlangen, Germany) following direct puncture of the portal vein with a 27-gauge needle (BD Microlance 3; Becton Dickinson GmbH, Heidelberg, Germany).
3
Air-pouch Implant Evaluation in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female C57BL/6 mice aged 8–12 weeks were purchased from Janvier Labs (Saint Berthevin, France). All mice were maintained on a standard diet with food and water ad libitum and a 12-h light-day-night rhythm. Under isoflurane anesthesia (0.6 mL/L oxygen and 2.5% isoflurane), 4 mL sterile air was injected into the shaved skin at the level of the scapulae of each mouse to create a dorsal subcutaneous air-pouch. The air-pouches were inflated with 2 mL of sterile air on days 2 and 4. On the fifth day, the biomaterial was implanted in a minimally invasive procedure under isoflurane anesthesia using an in-house, easy-to-use application system (Figures 1A, B ). This consisted of a 5 mL syringe (Discardit; Becton Dickinson, United States) and a 21 ½-G cannula (MicrolanceTM 3; Becton Dickinson, United States). The implant was inserted into the cannula under sterile conditions before being inserted into the air-pouch. The implants were left in the mice for 6 h to analyze the short-term response or for 10 days to analyze the long-term response (short-term: n = 6 animals per experimental group and time, long-term: n = 5 animals per control group and time). Animals receiving phosphate buffered saline (PBS) served as controls.
4
Protoplast Lysis and RNA Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The protoplasts of each sample (107 cells) were lysed by adding 9 mL lysis/binding buffer (500 mM LiCl, 0.5% (w/v) Lithium Dodecyl Sulphate (LiDS), 5 mM DTT, 20 mM Tris–HCl, pH 7.5, and 1 mM EDTA, pH 8.0) to the cell pellet resulting in a clear green solution. After homogenization by passing twice through a glass syringe (50 mL, FORTUNA® Optima®) with a narrow needle (0.9 × 25 mm, Becton–Dickinson microlanceTm 3) and incubation on ice for 10 min, the lysates were flash-frozen in liquid nitrogen and stored at −80 °C. Samples can be stored for up to 3 weeks.
5
Generation of Cold Atmospheric Plasma for Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An electronic device capable generating CAP into cell culture plates was developed at the Institute of Biophysics, Faculty of Medicine, University of Coimbra, as described in detail elsewhere [19 (link)]. Briefly, this device generates high voltage (4 kV) pulses with a frequency of 1 KHz through a sterilized needle with 0.9 mm of radius and 40mm of length (Microlance 3, Becton Dickinson, Franklin Lakes, NJ, USA). When charged, the needle works as an open-air single electrode CAP jet. An electrically grounded needle was submerged in the culture media. In this design, the cultures act both as target and grounded electrode, enabling plasma generation. The high voltage needle was placed 2mm above the surface of the cell culture’s medium. Cells were platted in 24-well plates (Sarstedt, Nümbrecht, Germany) at a density of 500,000 cells/mL in a volume of 500 μL per well and submitted to CAP treatment for short periods of time: 60 and 120 s. Further assays were performed 2 or 24 h after the exposure.
6
Tissue Sampling for Mitochondrial DNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A representative sample of each tissue was obtained by inserting a blunt end 19G 11/2 hypodermic needle (Microlance3, Becton Dickinson, Ireland) through the frozen tissue. DNA was extracted from the core sample by Blood & Cell Culture DNA Mini Kit (Qiagen, Valencia, California USA). DNA was eluted with elution buffer (as recommended by manufacturer) and stored at −20 °C. The DNA from these samples was analyzed for somatic mitochondrial mutations.
7
Shielded Vial and Syringe Handling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tests were performed in a class A shielded hood (Tema Sinergie).
To limit the risk that rubber cores would form, a Quincke needle (Vygon), 18-gauge and 90 mm in length, was put into the vial's cap using the stylet. The stylet was removed and an obturator top, IN-Stopper, was connected to the spinal needle. The system is represented in Figure 1. The dose was withdrawn into a syringe (Plastipak; BD Medical) connected to a needle, Microlance 3 (Becton Dickinson), 25 mm in length and 23-gauge, by piercing the rubber part of the connector. Shielded vials (Medisystem) weigh 915 g and shielded syringes (Medisystem) 172 g.
To limit the risk that rubber cores would form, a Quincke needle (Vygon), 18-gauge and 90 mm in length, was put into the vial's cap using the stylet. The stylet was removed and an obturator top, IN-Stopper, was connected to the spinal needle. The system is represented in Figure 1. The dose was withdrawn into a syringe (Plastipak; BD Medical) connected to a needle, Microlance 3 (Becton Dickinson), 25 mm in length and 23-gauge, by piercing the rubber part of the connector. Shielded vials (Medisystem) weigh 915 g and shielded syringes (Medisystem) 172 g.
8
Collecting and Culturing Mangrove Crab Embryos
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adult specimens of M. crozieri were collected in coastal mangrove areas in the Lower Florida Keys, USA in November 2014. Eggs without egg-shells (to produce ‘naked’ embryos) were obtained from adults by poking with a needle (BD Microlance 3) and raised in Petri dishes coated with 2 % agarose (diluted in filtered artificial seawater) or gelatin coated Petri dishes at room temperature in penicillin-streptomycin (100 μg/ml penicillin; 200 μg/ml streptomycin) treated Millipore filtered artificial seawater (35–36 ‰).
9
Subcutaneous Tumor Induction and Vaccination in C57BL/6 Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6 mice received 0.5 × 106 HPV-16 E7 expressing C3 cells [31 (link)] in 100 μl of PBS, subcutaneously in the right shaved flank (needles: 20G 1½” BD Microlance 3). When small tumours were palpable in all animals (12-15 days after tumour cell injection), the vaccine was injected i.m. in both musculus tibialis anterior for the DNA vaccines, or s.c. into the left flank for protein vaccines, as described above. Tumour sizes were measured with a caliper. Mice were sacrificed when the tumour size reached 400 mm2 or when tumours were bleeding. Tumour sizes of the mice within a group were calculated as arithmetic means with standard deviation (SD). All operations on live animals were performed under Isoflurane anaesthesia.
All animal experiments were performed with approval by and in accordance with regulatory guidelines and standards set by the institutional review board at Regierungspraesidium, Freiburg, Germany.
All animal experiments were performed with approval by and in accordance with regulatory guidelines and standards set by the institutional review board at Regierungspraesidium, Freiburg, Germany.
10
Preparation and Administration of Anti-TB Drugs
Check if the same lab product or an alternative is used in the 5 most similar protocols
M. tuberculosis H37Rv was grown in Middlebrook 7H9 broth (Difco, Detroit, MI, USA) supplemented with 10% oleic acid-albumin-dextrose-catalase (OADC; Life Technologies, Gaithersburg, MD, USA), 0.05% Tween 80, and 0.2% glycerol (VWR International GmbH, Darmstadt, Germany). Mid-exponential-phase cultures were harvested, aliquoted, and frozen at −80°C. After thawing, the bacterial suspension was drawn through a nonpyrogenic needle (Microlance3; BD, Drogheda, Ireland) to ensure proper dispersion of mycobacteria prior to aerosol infection. PZA, CFZ, and RIF were purchased from Sigma-Aldrich (Taufkirchen, Germany). RIF was ground to a small particle size before sterile water was added (73 (link)). PZA was dissolved in sterile water and incubated at 55°C until particles were dissolved. CFZ was prepared by grinding with a mortar and pestle and added to 0.05% (wt/vol) agarose dissolved in sterile water (51 (link)). Concentrated stocks of individual drugs were kept at 4°C and combined on the day of application. PZA solutions, if particulate, were heated to 55°C before combination. Daily drug dosages of 150 mg/kg for PZA, 25 mg/kg for CFZ, and 10 mg/kg for RIF were chosen to achieve human-equivalent doses (44 (link)).
+ Expand
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!