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2 protocols using tyrosin

1

Zinc Oxide Nanoparticle Assays

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Suspensions of n-ZnO (mean particle size 35 nm), reduced glutathione (GSH), bovine serum albumin, phenylmethylsulfonyl fluoride (PMSF), 5,5′-dithio-bis(2-nitrobenzoic acid) (DTNB), nicotinamide adenine dinucleotides (NADH, NAD, NADPH), EDTA, dihydrorhodamine, Hoescht 33342 dye, 2,4-Dinitrophenylhydrazine, tyrosin, hemoglobin, chymotrypsinogen, cytohrome c, myoglobin, ubiquitin, Sephadex G-50, β-mercaptoethanol, ethoxyresorufin, certified reference material ERM-BB422 and Lactobacillus leichmannii D-Lactate dehydrogenase were purchased from Sigma Chem. Co. (St. Louis, USA). All other chemicals were obtained from the Synbias (Kyiv, Ukraine), Bayer (Kyiv, Ukraine) and Balkanpharma-Dupnitsa (Dupnitsa, Bulgaria) commercial suppliers. All reagents were of the analytical grade or higher.
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2

Synthesis and Characterization of Novel Organic Compounds

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All the chemicals and reactants used in this study were of analytical grade. Methanol, Benzoin, vanillin, picric acid, 4-amino phenol, dichlorophenol, 4-hydroxy benzaldehyde, tyrosin, thymol, alpha-naphthol, and beta-naphthol were purchased from Sigma Chemicals (St. Louis, MO, USA). DPPH (2, 2-diphenyl-1-picrylhydrazyl) and salicylic acid were purchased from Merck (Darmstadt, Germany). 2-(N-((2′-(2H-tetrazole-5-yl)-[1,1′-biphenyl]-4yl)-methyl)-pentanamido)-3-methyl butanoic acid (96%, USP grade) was obtained from Highnoon laboratories Pvt. Ltd., Lahore, Pakistan. Using the Gallen Kamp melting point apparatus, melting points were recorded. 1H and 13C nuclear magnetic resonance analysis was performed. The cary630 FTIR spectrophotometer was used to generate a Fourier transform infrared (FTIR) spectrum using the KBr pellet press technique. The glassware was dried in a UN55 (Memmert) oven at 200 °C. In 1.0 mM of each reactant, the % yields of derivatives are provided.
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