Nod scid il2rγ null mice

Manufactured by Charles River Laboratories

Sourced in Italy, Germany

The NOD/SCID/Il2Rγ null mice are an immunodeficient mouse model with a severe combined immunodeficiency (SCID) phenotype. These mice lack T cells, B cells, and functional natural killer (NK) cells, making them highly susceptible to engraftment of human cells and tissues.

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6 protocols using nod scid il2rγ null mice

Xenograft Model of Human Cancer Stem Cells

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XTs were generated as previously described.^{32 (link)} CSCs (300 000 cells) were re-suspended in 100 μl of culture medium and Matrigel (1:1) (BD Pharmingen, Milan, Italy) and subcutaneously injected in the flanks of 6-week-old female NOD/SCID/IL2Rγnull mice (Charles River Laboratories, Calco, LC, Italy). Each experimental group consisted of six mice, allocated using a simple randomization method. Tumor volume (1/2(length × width²)) was assessed using digital caliper. Mice were treated with GANT61 (40 mg/kg, i.p.) re-suspended in ethanol and diluted to 100 μl per dose with 10% (2-hydroxypropyl)-β-cyclodextrin (vehicle) or vehicle alone (controls). Injections were administered twice a week for 4 weeks. All procedures were performed with the approval of the Ethics Committee for Animal Experimentation.

Po A., Silvano M., Miele E., Capalbo C., Eramo A., Salvati V., Todaro M., Besharat Z.M., Catanzaro G., Cucchi D., Coni S., Di Marcotullio L., Canettieri G., Vacca A., Stassi G., De Smaele E., Tartaglia M., Screpanti I., De Maria R, & Ferretti E. (2017). Noncanonical GLI1 signaling promotes stemness features and in vivo growth in lung adenocarcinoma. Oncogene, 36(32), 4641-4652.

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Engrafting NOD-SCID Mice for Research

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Non-obese diabetic/severe combined immunodeficiency (NOD-scidIL2Rγ^null) mice at 6 weeks old were purchased from Charles River laboratories (Wilmington, MA). Mice were maintained under specific pathogen free conditions, and experimental procedures were performed according to the protocol approved by Institutional Animal Care and Use Committee.

Sasaki H., Iyer S.V., Sasaki K., Tawfik O.W, & Iwakuma T. (2015). An improved intrafemoral injection with minimized leakage as an orthotopic mouse model of osteosarcoma. Analytical biochemistry, 486, 70-74.

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Genetic Mouse Models for Cancer Research

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Rosa26-YFP mice^{42 (link)}, Lgr5-cre^ER mice^{43 (link)}, Kras^LSL⁻^G12D mice^{44 (link)} and Trp53^fl/fl mice^{45 (link)} were imported from the NCI mouse repository and Jackson Laboratories. NOD/SCID/Il2Rγ null mice were purchased from Charles River. All mouse groups used in this study were composed of males and females with mixed genetic background. No randomization and no blinding were performed in this study. The Rosa26LSL-NTN1 transgenic mice (for LKPR-NTN1 gain of function) were imported from Mehlen Laboratory-Apoptosis, Cancer and Development, Centre de Recherche en Cancérologie, Lyon, France^{46 (link)}.

Lengrand J., Pastushenko I., Vanuytven S., Song Y., Venet D., Sarate R.M., Bellina M., Moers V., Boinet A., Sifrim A., Rama N., Ducarouge B., Van Herck J., Dubois C., Scozzaro S., Lemaire S., Gieskes S., Bonni S., Collin A., Braissand N., Allard J., Zindy E., Decaestecker C., Sotiriou C., Salmon I., Mehlen P., Voet T., Bernet A, & Blanpain C. (2023). Pharmacological targeting netrin-1 inhibits EMT in cancer. Nature, 620(7973), 402-408.

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Genetically Engineered Mouse Models

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Rosa26-YFP(Srinivasetal.,
2001), K14CreER (Vasioukhin
etal., 1999), Lgr5CreER (Barker et
al., 2007), KRas^LSL-G12D (Tuveson et al., 2004 (link)) and p53^fl/fl(Jonkers et al., 2001 (link)) mice have
been imported from the NCI mouse repository and the Jackson Laboratories.
Wim Declercq (Ghent University, Belgium) generated the
Rosa26-ΔNp63-IRES-GFP. NOD/SCID/Il2Rγ null mice were
purchased from Charles River.
All mice used in this study were composed of males and females with
mixed genetic background. Mouse colonies were maintained in a certified
animal facility in accordance with the European guidelines and with approved
ethical protocol (#483N).

Latil M., Nassar D., Beck B., Boumahdi S., Wang L., Brisebarre A., Dubois C., Nkusi E., Lenglez S., Checinska A., Drubbel A.V., Devos M., Declercq W., Yi R, & Blanpain C. (2016). Cell-Type-Specific Chromatin States Differentially Prime Squamous Cell Carcinoma Tumor-Initiating Cells for Epithelial to Mesenchymal Transition. Cell stem cell, 20(2), 191-204.e5.

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In vivo Tumor Formation Assay

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In vivo tumorigenic assays were performed in NOD/SCID IL2Rγnull mice (Charles River) by subcutaneously injecting 5 × 10⁶ cells into the flank region of the animals. Mice were housed under standard conditions at the CNC animal facility and screened twice a week for tumor formation. All animal procedures were conducted according to the EU Directive 2010/63/EU for animal experiments and reviewed and approved by DGAV, ORBEA and the animal facility ethics committee.

Rodrigues C.F., Serrano E., Patrício M.I., Val M.M., Albuquerque P., Fonseca J., Gomes C.M., Abrunhosa A.J., Paiva A., Carvalho L., Botelho M.F., Almeida L., Carreira I.M, & Alpoim M.C. (2018). Stroma-derived IL-6, G-CSF and Activin-A mediated dedifferentiation of lung carcinoma cells into cancer stem cells. Scientific Reports, 8, 11573.

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Ethanol-Induced Colitis Model in NOD/scid IL-2Rγ Mice

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NOD/scid IL-2Rγ^null mice were obtained from Charles River Laboratories (Sulzfeld, Germany). Mice were kept under specific pathogen-free conditions in individually ventilated cages in a facility controlled according to the Federation of Laboratory Animal Science Association guidelines. Following engraftment (day 1), mice were presensitized by rectal application of 150 µL of 10% ethanol on day 8 using a 1-mm cat catheter (Henry Schein, Hamburg, Germany). The catheter was lubricated with Xylocain Gel 2% (AstraZeneca, Wedel). Rectal application was performed under general anaesthesia using 4% Isofluran. Postapplication, mice were kept at an angle of 30° to avoid ethanol dripping. On days 15 and 18, mice were challenged by rectal application of 50% ethanol following the protocol of day 8. Mice were killed on day 21.
Mice were treated by intraperitoneal application of 30 µg of anti-CD1a antibody (Clone OKT6, BioXcell, West Lebanon, USA) or 30 µg of isotype control (Biolegend, San Diego, CA, USA) on days 7, 14, and 17.

Al-amodi O., Jodeleit H., Beigel F., Wolf E., Siebeck M, & Gropp R. (2018). CD1a-Expressing Monocytes as Mediators of Inflammation in Ulcerative Colitis. Inflammatory Bowel Diseases, 24(6), 1225-1236.

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