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2 protocols using terbium 3 acetate hydrate

1

DNA Oligonucleotide Functionalized Nanoparticles

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Gadolinium(III) acetate hydrate (99.9%), cerium(III) acetate hydrate (99.9%), terbium(III) acetate hydrate (99.9%), sodium hydroxide (NaOH, 98+%), ammonium fluoride (NH4F, 98+%), 1-octadecene (90%), oleic acid (90%), oleyamine (98+%), poly(acrylic acid) (PAA, MW ≈ 1800), phosphate-buffered saline (PBS), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (98+%) and N-hydroxysuccinimide (98%) were purchased from Sigma-Aldrich (Shanghai, China). Targeted DNA oligonucleotide (5′-TCCCTGGACTTGATCTGCTG-3′) and amine-modified capture DNA oligonucleotides (5′-AGTCCAGGGA-NH2-3′ and 5′-NH2-CAGCAGATCA-3′) were purchased from Sangon Biotech (Shanghai, China) Co., Ltd. All reagents were used as received without further purifications.
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2

Synthesis of Lanthanide-based Nanoparticles

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Yttrium (III) acetate hydrate, cerium (III) acetate hydrate, terbium (III) acetate hydrate, gadolinium (III) acetate hydrate (99.9%), oleic acid (OA, 90%), 1-octadecene (ODE, 90%), sodium hydroxide (NaOH), ammonium fluoride (NH4F), ethanol (absolute), methanol, cyclohexane, tetrahydrofuran (THF), dopamine hydrochloride, hydrochloric acid (HCl), peptone, L-cysteine, maltose, sodium phosphate dibasic (Na2HPO4), and tetrahydrofuran (THF, ≥97%) were purchased from Sigma-Aldrich (St Louis, Mo, USA). C. novyi-NT spores were kindly provided by BioMed Valley Discoveries (Kansas City, MO, USA). Lipiodol™ was obtained from Guerbet (Villepinte, France).
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