A hematology analyzer (Siemens, Germany, ADVIA2120i), a PCR system (ABI Company, USA, 7500), a Total RNA Extraction Kit, an EasyPure miRNA Kit, a Reverse transcription+PCR Kit, and the TransScript miRNA First-Strand cDNA Synthesis SuperMix (TransGen Biotech, Beijing, China, ER601-01, AT351-01) were used. The miR-21 primers were synthesized by Shanghai Sangon Biotech Co., Ltd. (Table 1 ).
Reverse transcription pcr kit
Manufactured by Transgene
Sourced in United States
The Reverse Transcription + PCR Kit is a laboratory tool that enables the conversion of RNA into complementary DNA (cDNA) and subsequent amplification of the cDNA through the polymerase chain reaction (PCR) process. The kit provides the necessary reagents and enzymes to perform these two critical steps in molecular biology workflows.
Automatically generated - may contain errors
2 protocols using reverse transcription pcr kit
1
Quantifying miR-21 Expression
2
Liver Gene Expression Analysis by qPCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was extracted from liver. RNA was used for cDNA synthesis using a reverse transcription PCR kit (TransGen Biotech). Real-time qPCR was performed using Tip Green qPCR SuperMix (TransGen Biotech) with 1:10 diluted cDNA product from the transcription product. GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) was used as housekeeping gene. Data was calculated according to 2ΔΔCt method. Primer sequence for targeted mouse gene was available in Table 1 .
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