Cd49c

Antibodies were selected based on expected differences in expression levels among the 5 cell lines. The antibodies that were used belong to three different categories providing information of tissue origin, recognizing antigens involved in cell adhesion and potential drug targets. Anti-human EpCAM/CD326, Her2 and EGFR antibody were provided by Immunicon Corp. (Huntingdon Valley, Philadelphia, USA). Anti-human CD3, CD8a, CD11c, CD14, CD19, CD20, CD25, CD33, CD45, CD56, CD61, CD66b, CD105, CD123, CD140a, CD146, CD235a, CD71, CD117, CD221, CD227, CD261, CD262, CD309, Her3, CD24, CD44, CD49a, CD49b, CD49c, CD49d, CD49e, CD49f, CD103, CD104, CD106, CD113, CD144, CD166, CD324, CD334 antibodies were purchased from Biolegend Inc. (San Diego, CA, USA). Anti-human CD113 was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Anti-human CD103 was purchased from BD Biosciences (San Jose, CA, USA). Anti-human serum albumin (HSA) antibody was used as a negative control in the SPRi experiments and was purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Anti-mouse IgG PE was purchased from Abcam (Cambridge, UK) and was used for staining of unlabeled antibodies in flow cytometry and as a negative control in the SPRi experiments.