Rabbit anti cd36 antibody

Manufactured by Proteintech

Sourced in United Kingdom, China

Rabbit anti-CD36 antibody is a laboratory reagent used for the detection and analysis of CD36 protein in various biological samples. CD36 is a cell surface receptor that plays a role in the recognition and internalization of oxidized low-density lipoproteins. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and flow cytometry to identify and study the expression of CD36 in different cell types and tissues.

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Anti-CD36 (8 citations) Anti-TM (2 citations)

3 protocols using rabbit anti cd36 antibody

Histological Analysis of Atherosclerotic Lesions

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Cardiac tissues were fixed in 10% buffered formalin for 30 min, dehydrated overnight in 75% ethanol, and embedded in paraffin. Serial sections (4 μm) were cut for morphometric analysis of the atherosclerotic lesions. The sections were stained with H&E, Masson’s trichrome, and Periodic Acid-Schiff (PAS) for histological analysis. For immunohistochemical staining, the heart sections were deparaffinized and rehydrated. Next, the sections were blocked with 3% H₂O₂ in methanol for 15 min to inactivate the endogenous peroxidases and incubated overnight at 4 ℃ with the primary antibodies: TGF-β (rabbit anti-TGF-β antibody, 1:300; Proteintech, Wuhan, China), collagen I (rabbit anti-collagen I antibody, 1:1000; Proteintech), collagen III (rabbit anti-collagen III antibody, 1:1000; Proteintech), MMP2 (rabbit anti- MMP2 antibody, 1:200; Proteintech), MMP9 (rabbit anti- MMP9 antibody, 1:300; Proteintech), LOX-1 (rabbit anti-LOX-1 antibody, 1:300; Abcam, England, CD36 (rabbit anti-CD36 antibody, 1:500; Proteintech). The sections were then incubated for 30 min at room temperature with a goat anti-rabbit HRP secondary antibody (anti-rabbit Universal Immunohistochemical Detection Kit; Proteintech). All sections were examined with an Olympus B×40 upright light microscope (Olympus, Tokyo, Japan).

Dong M., Luo Y., Lan Y., He Q., Xu L, & Pei Z. (2023). Luteolin reduces cardiac damage caused by hyperlipidemia in Sprague-Dawley rats. Heliyon, 9(6), e17613.

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Immunohistochemical Analysis of Cardiac Markers

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For immunohistochemical staining, the heart sections were deparaffinized and rehydrated. Next, the sections were blocked with 3% H₂O₂ in methanol for 15 min to inactivate the endogenous peroxidases and incubated overnight at 4°C with the primary antibodies: CD36 (rabbit anti-CD36 antibody, 1 : 200; Proteintech, Wuhan, China), SCD-1 (rabbit anti-SCD-1 antibody, 1 : 200; Abcam, England), PGC1-ɑ (rabbit anti-PGC1-ɑ antibody, 1 : 200; Proteintech), CPT-1 (rabbit anti-CPT-1 antibody, 1 : 200; Proteintech), p-AMPK (rabbit anti-p-AMPK antibody, 1 : 100; Proteintech), PPAR-α (rabbit anti-PPAR-α antibody, 1 : 50; Proteintech), BAX (rabbit anti-BAX antibody, 1 : 200; Proteintech), Bcl-2 (rabbit anti-Bcl-2 antibody, 1 : 200; Proteintech), and cleaved caspase-3 (rabbit anti-cleaved caspase-3 antibody, 1 : 400; Cell Signaling Technology, USA). The sections were incubated with goat anti-rabbit HRP secondary antibody (Histofine Simple Stain Kit; Nichirei, Tokyo, Japan) for 30 min at room temperature. All sections were examined using an Olympus light microscope (Olympus, Tokyo, Japan).

Pei Z., Wang X., Yang C., Dong M, & Wang F. (2021). Recombinant Human Growth Hormone Inhibits Lipotoxicity, Oxidative Stress, and Apoptosis in a Mouse Model of Diabetic Cardiomyopathy. Oxidative Medicine and Cellular Longevity, 2021, 3899356.

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Molecular Mechanisms of Atherosclerosis

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The materials and reagents were the following: total cholesterol, low-density lipoprotein-cholesterol, and high-sensitivity C-reactive protein assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China); IL-6 and TNF-α ELISA kit (Proteintech, Wuhan, China); immunohistochemistry staining kit (Zsbio, Beijing, China); primary antibodies against CD68 (rabbit anti-CD68 antibody, 1 : 200; Proteintech, Wuhan, China); CD36 (rabbit anti-CD36 antibody, 1 : 200; Proteintech); polyvinylidene fluoride membranes (Immobilon, Millipore, Billerica, MA, USA); diluent (P0023A; Beyotime); NOD-like receptor 3 (NLRP3; rabbit anti-NLRP3 antibody, 1 : 1000; Boster, Wuhan, China); interleukin- (IL-) 18 (rabbit anti-IL-18 antibody, 1 : 1000; Proteintech); IL-1β (rabbit anti-IL-1β antibody, 1 : 1000; Arigo, Hamburg, Germany); caspase-1 (rabbit anti-caspase-1 antibody, 1 : 1000; Proteintech); phosphoextracellular signal-related kinase (P-ERK; rabbit anti-P-ERK, 1 : 1000; Proteintech); NF-κB (NF-κB; rabbit anti-NF-κB, 1 : 1000; Proteintech); p-38 (p-38; rabbit anti-p-38, 1 : 1000; Proteintech); anti-β-actin (1 : 1000; Proteintech); secondary antibody (anti-rabbit Ig-G, 1 : 1000; Cell Signaling Technology); cDNA Synthesis SuperMix kit (Transgen, Beijing, China); qPCR; and the TransStart Top Green qPCR SuperMix kit (Transgen).

Pei Z.W., Guo Y., Zhu H.L., Dong M., Zhang Q, & Wang F. (2020). Thymoquinone Protects against Hyperlipidemia-Induced Cardiac Damage in Low-Density Lipoprotein Receptor-Deficient (LDL-R−/−) Mice via Its Anti-inflammatory and Antipyroptotic Effects. BioMed Research International, 2020, 4878704.

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