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Anti cd115 csf 1r pe

Manufactured by Thermo Fisher Scientific
Sourced in United States

Anti-CD115(CSF-1R)-PE is a fluorescently labeled antibody that binds to the CD115 (CSF-1R) surface antigen. It can be used for the identification and analysis of cells expressing CD115 by flow cytometry.

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3 protocols using anti cd115 csf 1r pe

1

Immunophenotyping of AML mouse cells

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Bone marrow cells from AML mice were preincubated with rat IgG, and then incubated on ice with anti-CD115(CSF-1R)-PE (eBioscience San Diego, CA, USA) and anti-c-Kit-APC (2B8)-APC (BD Pharmingen San Jose, CA, USA). Flow cytometric analysis and cell sorting were carried out using the cell sorter JSAN (Baybioscience Kobe, Japan), and the results were analyzed using FlowJo software (Tree Star Ashland, OR, USA)).
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2

Characterization of AML Leukemic Cells

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BM cells from AML mice were preincubated with rat IgG, and then incubated on ice with anti-CD115(CSF-1R)-PE (eBioscience) and anti- c-Kit-APC (2B8)-APC (BD Pharmingen). Flow cytometric analysis and cell sorting were performed using the cell sorter JSAN (Baybioscience), and the results were analyzed using FlowJo software (Tree Star).
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3

Multicolor Flow Cytometry Analysis

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Bone-marrow cells from leukemic mice were pre-incubated with rat IgG (Sigma-Aldrich), and then incubated on ice with the appropriate staining reagents: anti-CD115(CSF1R)-PE (eBioscience, San Diego, CA, USA), anti-Mac-1(M1/70)-PE-Cy7 (eBioscience), anti-Gr-1(RB6-8C5)-APC (BD Pharmingen, San Diego, CA, USA) and anti-c-Kit(2B8)-APC-eF780 (eBioscience). FACS analysis and cell sorting were performed using the JSAN cell sorter and the results were analyzed using the flowjo software (Tree Star, Standford, CA, USA).
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