Acetylthiocholine (ASCh) iodide, butyrylthiocholine (BSCh) iodide, 5,5′-dithio-bis (c-nitrobenzoic acid) (DTNB), eserine hemisulfate salt, tetraisopropylpyrophosphoramide (iso-OMPA), 1,5-bis (4-allyldimethyl ammoniumphenyl)-pentan-3-one dibromide (BW284C51), HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid), EDTA (etilen diamino-tetracetic acid), boric acid, bovine serum albumin, Tris, glycerol, bromophenol blue, maleic acid, sodium citrate dihydrate, copper pentahydrate, potassium ferricyanide, cholinesterase acetyl (True Cholinesterase EC 3.1.1.7) Type V-S from Electric Eel, acrylamide, tetramethylenediamine and ammonium persulfate were purchased from SIGMA. Sodium dodecyl sulphate (99% pure) and ethanol (99% pure) were purchased from MERCK (Germany). Bisacrylamide was acquired from PROMEGA.
Tetramethylenediamine
Manufactured by Merck Group
Tetramethylenediamine is a chemical compound with the formula (CH2)4(NH2)2. It is a clear, colorless liquid with a characteristic amine odor. The primary function of tetramethylenediamine is as a chemical intermediate in the production of various compounds, including pharmaceuticals, polymers, and other industrial materials.
Automatically generated - may contain errors
Lab products found in correlation
Ammonium persulfate, by Merck Group (3 mentions)
Bis acrylamide, by Promega (1 mentions)
Acrylamide, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Sodium dodecyl sulphate (sds), by Merck Group (1 mentions)
Bis acrylamide, by Merck Group (1 mentions)
Acryloyl chloride, by Merck Group (1 mentions)
N methylpyrrolidone, by Merck Group (1 mentions)
Triethylamine, by Merck Group (1 mentions)
N isopropylacrylamide, by Merck Group (1 mentions)
N n methylenebisacrylamide, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Chempur (1 mentions)
Acetic acid, by Chempur (1 mentions)
Potassium hydroxide, by Chempur (1 mentions)
Sodium hydroxide (naoh), by Chempur (1 mentions)
Octanal, by Merck Group (1 mentions)
Methanol, by Chempur (1 mentions)
Hexamethylenediamine, by Merck Group (1 mentions)
1 iodopropane, by Merck Group (1 mentions)
1 iodododecane, by Merck Group (1 mentions)
4 protocols using tetramethylenediamine
1
Acetylcholinesterase Activity Assay
2
SDS-PAGE Analysis of Protein Fractions
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Electrophoresis of a concentrated fraction (in quantities of 1, 5 and 10 µl)
was carried out in polyacrylamide gel (PAAG-gel) (sodium dodecyl sulfate
(SDS–electrophoresis) by Laemli).37 (link) PAAG gel was prepared immediately before the experiment using the
acrylamide (Sigma-Aldrich) and bisacrylamide (Sigma-Aldrich) solutions.
Ammonium persulfate (Sigma-Aldrich) was used as a polymerization initiator
and tetramethylenediamine (Sigma-Aldrich) as a catalyst. We used the Spectra
(Spectra™ Multicolor Broad Range Protein Ladder) marker.Figure 1 shows clearly
two major protein compounds. Two strips corresponding to the marker scale in
the range of 140 to 260 and 50 kDa were cut out for identification.
was carried out in polyacrylamide gel (PAAG-gel) (sodium dodecyl sulfate
(SDS–electrophoresis) by Laemli).37 (link) PAAG gel was prepared immediately before the experiment using the
acrylamide (Sigma-Aldrich) and bisacrylamide (Sigma-Aldrich) solutions.
Ammonium persulfate (Sigma-Aldrich) was used as a polymerization initiator
and tetramethylenediamine (Sigma-Aldrich) as a catalyst. We used the Spectra
(Spectra™ Multicolor Broad Range Protein Ladder) marker.
two major protein compounds. Two strips corresponding to the marker scale in
the range of 140 to 260 and 50 kDa were cut out for identification.
3
Synthesis of Thermoresponsive Cyclodextrin Hydrogels
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Ammonium persulfate (APS, MW = 228.20), N-isopropylacrylamide (NIPAAm, MW = 113.16), N,N′-methylene-bis-acrylamide (MBA, MW = 154.17), tetramethylenediamine (TEMED, MW = 116.20, d = 0.775 g/ml);triethylamine (MW = 101.19, d = 0.796 g/mL) acryloyl chloride (MW = 90.51, d = 1.114 g/mL) were purchased from Sigma Aldrich. N-methyl pyrrolidone (NMP) was purchased from Sigma-Aldrich and distilled on molecular sieves before use. γ-cyclodextrin (γ-CD, MW: 972.89) was kindly supplied by IMCD Italia S.p.A. Mono-6-amino-deoxy-6-γ-cyclodextrin was obtained according to the literature (MW: 971.89, Tang and Ng, 2008 (link)).
4
Synthesis of Alkylated Amines
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D-glucose, tetramethylenediamine, hexamethylenediamine, iodoethane, 1-iodopropane, 1-iodododecane were purchased from Sigma-Aldrich. Hexanal was obtained from Fluka. Octanal, decanal, dodecanal, sodium borohydride and sodium cyanoborohydride were purchased from Merck. Sodium hydroxide, potassium hydroxide, hydrochloric acid, acetic acid, methanol, 2-propanol, N,N-dimethylformamide and diethyl ether were obtained from Chempur. Nitromethane was purchased from POCH. All reagents and solvents were used as obtained without further purification.
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