Pci6731 card

Manufactured by National Instruments

Sourced in United States

The PCI6731 card is a data acquisition device from National Instruments. It features analog output channels and digital I/O capabilities. The card is designed for use in various applications that require precise control and monitoring of signals.

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Lab products found in correlation

Ed1 electrostatic speaker driver, by Tucker-Davis Technologies (2 mentions) Labview 2014, by National Instruments (1 mentions) Usb 6361, by National Instruments (1 mentions)

2 protocols using pci6731 card

Auditory Neuron Frequency Response Characterization

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Auditory stimuli were delivered through a free-field ES1 speaker using an ED1 electrostatic speaker driver (Tucker Davis Technologies, USA). The speaker was placed ~6 cm from the mouse ear that was contralateral to the recorded AUD^{49 (link)–51 (link)}. The auditory stimuli were generated by a custom LabVIEW 2016 programme (National Instruments, USA) and converted to analogue voltages with a PCI6731 card (National Instruments, USA). A pre-polarized condenser microphone (377A01 microphone, PCB Piezotronics Inc., USA) was used to calibrate the generated sounds. To establish the FRA of each neuron, sequences of pure tones (50 ms duration) consisting of 5 sound levels (30–80 dB sound pressure level (SPL), 10 dB attenuation), and 11 frequencies (logarithmic scale from 2 to 40 kHz) were presented with 8 repetitions and randomized intervals of 2–4 s. During auditory stimulation, no other sensory stimulus was present to the mouse.

Wang M., Liu K., Pan J., Li J., Sun P., Zhang Y., Li L., Guo W., Xin Q., Zhao Z., Liu Y., Zhou Z., Lyu J., Zheng T., Han Y., Zhang C., Liao X., Zeng S., Jia H, & Chen X. (2022). Brain-wide projection reconstruction of single functionally defined neurons. Nature Communications, 13, 1531.

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Broadband Noise Stimulus Calibration

Cited 1 time

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The sounds were broadcasted by a free-field ES1 speaker with an ED1 electrostatic speaker driver (Tucker Davis Technologies, USA).⁵³ (link) The broadband noise (BBN, bandwidth 0-50 kHz, duration 50 ms) was generated by a custom-written software based on LabVIEW 2014 (National Instruments, USA) and transduced to an analog voltage by a PCI 6731 card (National Instruments, USA). During experiments, the speaker was put at a distance of ∼6 cm to the left ear of the animal. All sound levels were calibrated with a ¼-inch pressure pre-polarized condenser microphone system (377A01 microphone, 426B03 pre-amplifier, 480E09 signal conditioner, PCB Piezotronics Inc, USA). Data were sampled at 1 MHz via a high-speed data acquisition board USB-6361 from National Instruments and analyzed using custom-written software in LabVIEW 2014. The BBN was applied at ∼a 65 dB sound pressure level (SPL). The background noise (∼55 dB SPL) was below the BBN stimulation level.

Gu M., Li X., Liang S., Zhu J., Sun P., He Y., Yu H., Li R., Zhou Z., Lyu J., Li S.C., Budinger E., Zhou Y., Jia H., Zhang J, & Chen X. (2023). Rabies virus-based labeling of layer 6 corticothalamic neurons for two-photon imaging in vivo. iScience, 26(5), 106625.

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