Anti actin a5060

Manufactured by Merck Group

Sourced in United States

Anti-actin (A5060) is a mouse monoclonal antibody that recognizes the actin protein. It is a widely used tool in cell biology and biochemistry research.

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Lab products found in correlation

Anti phospho sapk jnk, by Cell Signaling Technology (1 mentions) Alexa fluor 488 conjugated goat anti mouse igg antibody, by Thermo Fisher Scientific (1 mentions) Anti phospho p38 mapk, by Cell Signaling Technology (1 mentions) Anti nf kb p65 c 20 sc 372, by Santa Cruz Biotechnology (1 mentions) Anti lamin a c sab4200236, by Merck Group (1 mentions) Anti acetyl histone h3 antibody 06 599, by Merck Group (1 mentions) Anti stat3 sc 482, by Santa Cruz Biotechnology (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) Piroxicam, by Merck Group (1 mentions) 5z 7 oxozeaenol, by Merck Group (1 mentions) Anti mmp9 ab76003, by Abcam (1 mentions) Hiperfect, by Qiagen (1 mentions) Bcl 2 13 8800, by Thermo Fisher Scientific (1 mentions) Anti gfap sc 33673, by Santa Cruz Biotechnology (1 mentions) Trichostatin a tsa, by MedChemExpress (1 mentions) Rt reagent kit, by Takara Bio (1 mentions) Premix ex taq, by Takara Bio (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Vorinostat, by MedChemExpress (1 mentions)

Close spelling variants of this product

Anti-β-actin (A5060) A5060 Anti-actin

5 protocols using anti actin a5060

Antibody Sources for Immunoblotting

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A monoclonal antibody against E-selectin (clone 7A9) was obtained from the American Type Culture Collection; anti-E-selectin (A-10: sc-137203) and anti-NF-kB p65 (C-20: sc-372) antibodies were obtained from Santa Cruz Biotechnology; anti-phospho-NF-κB p65 Ser 536 (#3031), anti-SAPK/JNK (#9252), anti-p38 MAPK (#9212), anti-phospho-SAPK/JNK (#9251), and anti-phospho-p38 MAPK (#4511) antibodies were obtained from Cell Signaling Technology; anti-lamin A/C (SAB4200236) and anti-actin (A5060) antibodies were obtained from Sigma-Aldrich; an anti-α-tubulin antibody (PM054-7) was obtained from Medical & Biological Laboratories; an anti-acetyl-histone H3 antibody (06-599) was obtained from Millipore; an Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (A11017) was obtained from Life Technologies; horseradish peroxidase (HRP)-linked anti-mouse (NA931V) and anti-rabbit (NA9340V) secondary antibodies were obtained from GE Healthcare.

Hamada K., Osaka M, & Yoshida M. (2014). Cell Density Impacts Epigenetic Regulation of Cytokine-Induced E-Selectin Gene Expression in Vascular Endothelium. PLoS ONE, 9(4), e90502.

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Immunoblotting Whole-cell Protein Analysis

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Whole-cell proteins were recovered from protein-rich flow-through solutions after the first centrifugation step of the RNeasy Mini Kit (Qiagen) used for total RNA extraction, as previously described8 (link). Proteins were then immunoblotted by standard procedures using the following antibodies: anti-IκBζ rabbit pAb (#9244) and anti-phospho-STAT3 (Tyr705) rabbit pAb (#9131) from Cell Signaling (Beverly, MA, USA); rabbit pAb anti-STAT3 (sc-482, Santa Cruz Biotechnology, Dallas, TX, USA); rabbit pAb anti-actin (A5060) from Sigma. Blotted proteins were detected and quantified by using the Odyssey infrared imaging system (LI-COR Biosciences, Lincoln, NE, USA). Densitometry values were calculated by subtracting, for each lane, the respective background levels.

Zimmermann M., Arruda-Silva F., Bianchetto-Aguilera F., Finotti G., Calzetti F., Scapini P., Lunardi C., Cassatella M.A, & Tamassia N. (2016). IFNα enhances the production of IL-6 by human neutrophils activated via TLR8. Scientific Reports, 6, 19674.

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TAK1 Inhibitor Signaling Pathway

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TAK1 inhibitors (5Z)‐7‐Oxozeaenol (EMD Millipore, Billerica, MA), Dexamethasone, Piroxicam (Sigma, St. Louis, MO) were purchased and reconstituted as per the manufacturer's instructions. Antibodies used in this study were as follows: anti‐actin (A 5060) (Sigma); anti‐phospho‐TAK1 (06‐1425) (Millipore, Temecula, CA); anti‐phospho‐TAK1 (109404) (Abcam, Cambridge, MA); anti‐TAK1 (5206, 4505), anti‐phospho‐SAPK/JNK (4668, 9251), anti‐SAPK/JNK (9253, 9252), anti‐phospho‐p38 MAPK (4511, 9211), anti‐p38 MAPK (9211, 9212), anti‐phospho‐IκBα (2859) and anti‐IκBα (4814, 9242) (Cell Signaling Technology, Beverly, MA); anti‐TAK1 (7162) (Santa Cruz Biotechnology, Santa Cruz, CA).

Liu Z., Kong F., Vallance J.E., Harmel‐Laws E., Amarachintha S., Steinbrecher K.A., Rosen M.J, & Bhattacharyya S. (2017). Activation of TGF‐β activated kinase 1 promotes colon mucosal pathogenesis in inflammatory bowel disease. Physiological Reports, 5(7), e13181.

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Molecular Regulation of MMP-1 and CCR2 Expression

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SiRNAs (CCR2/p65/scrambled) were from Santa Cruz Biotechnology and siRNA transfection reagent Hiperfect were obtained from Qiagen. RT‐PCR reagents and enzymes were from Invitrogen. Monoclonal antibodies, anti‐human MMP‐1 (MAB901), anti‐human CCR2 (MAB180) were from R&D Systems, UK, anti‐MMP‐9 (ab76003) from Abcam, anti‐phospho‐PKC (A‐11) from Santa Cruz and anti‐actin (A5060) from Sigma‐Aldrich. Human MCP‐1 ELISA kit (DuoSet) and Go 6976 (PKC inhibitor) were from R&D Systems. All other substances and materials were from Sigma‐Aldrich, Invitrogen, Life Technologies, Eppendorf, Bio‐Rad.

Macarie R.D., Vadana M., Ciortan L., Tucureanu M.M., Ciobanu A., Vinereanu D., Manduteanu I., Simionescu M, & Butoi E. (2018). The expression of MMP‐1 and MMP‐9 is up‐regulated by smooth muscle cells after their cross‐talk with macrophages in high glucose conditions. Journal of Cellular and Molecular Medicine, 22(9), 4366-4376.

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Histone Deacetylase Inhibitors Modulate Cell Signaling

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Vorinostat (SAHA) and Trichostatin A (TSA) were purchased from MedChem Express (Shanghai, China). Anti-GFAP (sc-33673) was purchased from Santa Cruz (Dallas, TX, USA). Anti-α-SMA (A5228), Anti-Desmin (D1033), Anti-acetyl-Histone H3 (H9286), Anti-acetyl-Histone H4 (SAB5600021) and Anti-actin (A5060) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-Cleaved Caspase-9 (9507), Anti-Cleaved Caspase-3 (5A1E), Anti-Smad5 (12534) were purchased from Cell Signaling (Beverly, MA, USA). Anti-Smad7 (42-0400) and Bcl-2 (13-8800) were purchased from Invitrogen (Carlsbad, CA, USA). RT reagent kit and Premix Ex Taq were from TAKARA (Dalian, China). Lipofectamine 2000 was purchased from Invitrogen (Carlsbad, CA, USA). Luciferase kit was purchased from Promega (WI, USA). Apoptosis kit was purchased from BD Biosciences (San Jose, CA, USA).

Ji T., Feng W., Zhang X., Zang K., Zhu X, & Shang F. (2020). HDAC inhibitors promote pancreatic stellate cell apoptosis and relieve pancreatic fibrosis by upregulating miR-15/16 in chronic pancreatitis. Human Cell, 33(4), 1006-1016.

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