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Cd115 clone afs98

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The CD115 (clone AFS98) is a lab equipment product offered by Thermo Fisher Scientific. It is a reagent used for the detection and analysis of CD115 protein expression. The core function of this product is to provide a tool for researchers to study the CD115 protein, which is a receptor that plays a role in various biological processes.

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Lab products found in correlation

Cd11b clone m1 70, by Thermo Fisher Scientific (4 mentions) F4 80 clone ci a3 1, by Bio-Rad (2 mentions) Mhc 2 clone m5 114, by Thermo Fisher Scientific (2 mentions) Gr 1 clone rb6 8c5, by BioLegend (2 mentions) Cd45 clone 30 f11, by Thermo Fisher Scientific (2 mentions) Cd3e clone 145 2c11, by BioLegend (2 mentions) Lsrii fortessa, by BD (2 mentions) Ly6c clone hk1.4, by Thermo Fisher Scientific (2 mentions) Ly6g 1a8, by BioLegend (1 mentions) Cd19 clone 1d3, by BD (1 mentions) Facs aria 2 or 3, by BD (1 mentions) Cd11b clone m1 70, by BD (1 mentions) Cd11c clone n418, by BioLegend (1 mentions) 7 aad, by BD (1 mentions) Fc block, by BD (1 mentions) Cd45 clone 30 f11, by BD (1 mentions) Ly6g clone 1a8, by BD (1 mentions) Ack lysis buffer, by Thermo Fisher Scientific (1 mentions) F4 80 clone bm8, by Thermo Fisher Scientific (1 mentions) Cd45.2 clone 104, by BioLegend (1 mentions)

5 protocols using cd115 clone afs98

1

Multicolor Flow Cytometry Immunophenotyping

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For flow cytometry or cell sorting, cells were transferred to 15-mL tubes and washed in cold buffer (2% FCS, 2 mM EDTA in PBS). Before antibody labeling, cells were incubated with Fc-Block (1:200, anti-CD16/32 antibody; 2.4G2, BD Pharmingen) for 10 min at 4°C and washed. Cells were stained in buffer with (1:100) fluorescently labeled antibodies against CD11b (clone M1/70, BD Pharmingen), CD11c (clone N418, Biolegend), CD115 (clone AFS98, eBioscience), CD19 (clone 1D3, BD Pharmingen), and Ly-6G (1A8, Biolegend) for 30 min at 4°C in the dark. 7-AAD (BD Pharmingen) was added before measurement to exclude dead cells. Analysis was performed on LSRFortessa and sorting on FACSAria II or III (BD Pharmingen). Unstained, empty vector-transduced cells or fluorescence-minus-one staining setups served as controls.
Cirovic B., Schönheit J., Kowenz-Leutz E., Ivanovska J., Klement C., Pronina N., Bégay V, & Leutz A. (2017). C/EBP-Induced Transdifferentiation Reveals Granulocyte-Macrophage Precursor-like Plasticity of B Cells. Stem Cell Reports, 8(2), 346-359.
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2

Isolation and Characterization of Microglia

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Mice were deeply anesthetized with a lethal dose of choral hydrate and transcardially perfused with 0.1 M PBS. Brain was removed and one forebrain hemisphere (without olfactory bulb) was minced, incubated in phenol-red free DMEM supplemented with 2% heat inactivated FBS, 10mM HEPES and Collagenase type IV (0.4 mg/mL) for 15 min and then passed through a 19G blunt syringe to obtain a homogeneous cell suspension. Mononuclear cells were separated with a 40% Percoll gradient. Isolated cells were surface stained in FACS buffer for 20–30 min on ice with the following antibodies: CD11b (clone M1/70, eBioscience), F4/80 (clone CI: A3-1, BioRad), CD45 (clone 30F11, eBioscience), MHC II (clone M5/114.15.2, eBioscience), CD3e (clone 145-2C11, Biolegend), Gr-1 (clone RB6–8C5, Biolegend), CD115 (clone AFS98, eBioscience). Multiparameter analysis was performed on a LSR II Fortessa (BD) and analyzed with FlowJo software (Tree Star) (Details are included in the Flow Cytometry Reporting Summary). Dead cells and doublets were excluded from all analysis.
Menard C., Pfau M.L., Hodes G.E., Kana V., Wang V.X., Bouchard S., Takahashi A., Flanigan M.E., Aleyasin H., LeClair K.B., Janssen W.G., Labonté B., Parise E.M., Lorsch Z.S., Golden S.A., Heshmati M., Tamminga C., Turecki G., Campbell M., Fayad Z., Tang C.Y., Merad M, & Russo S.J. (2017). Social stress induces neurovascular pathology promoting depression. Nature neuroscience, 20(12), 1752-1760.
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3

Isolation and Characterization of Microglia

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Mice were deeply anesthetized with a lethal dose of choral hydrate and transcardially perfused with 0.1 M PBS. Brain was removed and one forebrain hemisphere (without olfactory bulb) was minced, incubated in phenol-red free DMEM supplemented with 2% heat inactivated FBS, 10mM HEPES and Collagenase type IV (0.4 mg/mL) for 15 min and then passed through a 19G blunt syringe to obtain a homogeneous cell suspension. Mononuclear cells were separated with a 40% Percoll gradient. Isolated cells were surface stained in FACS buffer for 20–30 min on ice with the following antibodies: CD11b (clone M1/70, eBioscience), F4/80 (clone CI: A3-1, BioRad), CD45 (clone 30F11, eBioscience), MHC II (clone M5/114.15.2, eBioscience), CD3e (clone 145-2C11, Biolegend), Gr-1 (clone RB6–8C5, Biolegend), CD115 (clone AFS98, eBioscience). Multiparameter analysis was performed on a LSR II Fortessa (BD) and analyzed with FlowJo software (Tree Star) (Details are included in the Flow Cytometry Reporting Summary). Dead cells and doublets were excluded from all analysis.
Menard C., Pfau M.L., Hodes G.E., Kana V., Wang V.X., Bouchard S., Takahashi A., Flanigan M.E., Aleyasin H., LeClair K.B., Janssen W.G., Labonté B., Parise E.M., Lorsch Z.S., Golden S.A., Heshmati M., Tamminga C., Turecki G., Campbell M., Fayad Z., Tang C.Y., Merad M, & Russo S.J. (2017). Social stress induces neurovascular pathology promoting depression. Nature neuroscience, 20(12), 1752-1760.
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4

Dissection and Flow Cytometric Analysis of Immune Cells

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Whole blood was collected via cardiac puncture from euthanized animals. Red blood cells were lysed using ACK lysis buffer (Fisher, Cat#A1049201), and then the remaining cells were stained with fluorochrome-conjugated antibodies. Skin tissues were digested as described in the protocol above. Single cell suspensions were stained with fluorochrome-conjugated antibodies. Data were analyzed by FlowJo v10.8 (DB Life Sciences). The following antibodies and dyes were used: CD45 (clone 30-F11; BD Biosciences Cat# 564279), CD11b (clone M1/70; Thermo Fisher Scientific Cat# 45–0112-80), CD115 (clone AFS98; Thermo Fisher Scientific Cat# 12–1152-81), Ly6C (clone HK1.4; Thermo Fisher Scientific Cat# 47–5932-80), Ly6G (clone 1A8, BD Biosciences Cat# 565369), F4/80 (clone BM8, Thermo Fisher Scientific Cat# 17–4801-80), Live/Dead Aqua or Violet fixable stains (Life: L34964, NC0180395).
An L., Kim D., Donahue L., Mejooli M.A., Chi-Yong E., Nishimura N, & White A.C. (2023). Sexual dimorphism in melanocyte stem cell behavior reveals combinational therapeutic strategies for cutaneous repigmentation. bioRxiv.
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5

Immune cell isolation and analysis

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Graft tissue was digested and single-cell suspensions were prepared as previously reported (6 (link)). Following red blood cell lysis, cells were stained with fluorochrome-labeled antibodies against CD45.2 (clone 104; Biolegend), CD45.1 (clone A20; Biolegend), Ly6G (clone 1A8; Thermo Fisher Scientific), CD11b (clone M1/70; Thermo Fisher Scientific), Ly6C (clone HK1.4; Thermo Fisher Scientific), CD115 (clone AFS98; Thermo Fisher Scientific), and isotype control antibodies (BD Biosciences; BioLegend). Data were acquired on an Attune NxT Acoustic Focusing Cytometer (Thermo Fisher Scientific) and analyzed with FlowJo v10 software.
Li W., Shepherd H.M., Terada Y., Shay A.E., Bery A.I., Gelman A.E., Lavine K.J., Serhan C.N, & Kreisel D. (2023). Resolvin D1 prevents injurious neutrophil swarming in transplanted lungs. Proceedings of the National Academy of Sciences of the United States of America, 120(31), e2302938120.
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