Dulbecco’s modified Eagles’ medium (DMEM), penicillin/streptomycin, trypsin/EDTA, fetal bovine serum (FBS), KGM-SFM medium, and phosphate-buffered saline (PBS) were from Gibco (Life Technologies, USA). Arecoline, melatonin and MTT were from Sigma (Sigma-Aldrich Chemical Company, St. Louis, MO, USA). ELISA kits for MMP-9 were from PeproTech (PeproTech, Inc., Rocky Hill, NJ, USA). Catalase, SB431542, 5Z-7-Oxozeaenol, PD153035, AG490, U0126, LY294002, and aspirin were purchased from Tocris. Phospho-TAK1 (p-TAK1) antibody was from Abcam (ab79583). Antibodies for TGF-β1, and GAPDH were from Santa Cruz. P-Smad2 antibody was from Cell Signaling Technology. PBL extract and ANE were prepared as before [8 (link), 12 (link), 13 (link), 21 (link)]. HC was synthesized as previously [22 (link)].
Aspirin
Manufactured by Bio-Techne
Sourced in China, United Kingdom
Aspirin is a chemical compound used in various laboratory applications. It is a white, crystalline solid with a melting point of 135°C. Aspirin is commonly used as a reference standard, a reagent in chemical reactions, and in the analysis of other compounds.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Melatonin, by Merck Group (1 mentions)
5z 7 oxozeaenol, by Bio-Techne (1 mentions)
Sb431542, by Bio-Techne (1 mentions)
Pd153035, by Bio-Techne (1 mentions)
Ag490, by Bio-Techne (1 mentions)
P smad2 antibody, by Cell Signaling Technology (1 mentions)
Arecoline, by Merck Group (1 mentions)
Ly294002, by Bio-Techne (1 mentions)
U0126, by Bio-Techne (1 mentions)
Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions)
Aceq qpcr sybr green master mix, by Vazyme (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Taq master mix, by Vazyme (1 mentions)
Az10606120, by Bio-Techne (1 mentions)
3 protocols using aspirin
1
Molecular Mechanisms Regulating Cell Signaling
2
Purinergic Signaling in Mast Cell Activation
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ATP (Sigma-Aldrich, St. Louis, MO, United States), PPADS (10 µM, Tocris Bioscience, Missouri, USA, a nonselective P2 purinergic receptor antagonist), NF449 (1 µM, Cayman, Ann Arbor, Michigan, USA, P2 × 1 receptor antagonist), 5-BDBD (1 µM, Sigma-Aldrich, St. Louis, MO, United States, P2 × 4 receptor antagonist), AZ10606120 (1 µM, Tocris Bioscience, Missouri, USA, P2 × 7 receptor antagonist), recombinant mouse SCF protein (10 ng/mL, R&D Systems, Minneapolis, MN, USA), Penicillin and streptomycin (100 µg/mL; Gibco, Waltham, MA, USA), bronectin (30 µg/mL; Sigma-Aldrich, St. Louis, MO, United States), Fluo 4-AM (Solarbio, Beijing, China, calcium indicator), Histamine ELISA Kit (Yifeixue, Nanjing, China), Trizol (Vazyme Biotech, Nanjing, China), HiScript II Q RT SuperMix for qPCR (Vazyme Biotech, Nanjing, China), Taq MasterMix (Vazyme Biotech, Nanjing, China), AceQ qPCR SYBR Green Master Mix (Vazyme Biotech, Nanjing, China), salicylic acid (300 µM, Yuanye Biotech, China), aspirin (1 Mm, Tocris Bioscience, Missouri, USA).
3
Lubiprostone Modulates Cytokine Responses
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Lubiprostone was purchased from Abcam plc (Cambridge, UK). IFNγ, IL-6, and IL-1β were purchased from R&D Systems (Minneapolis, MN, USA). Aspirin was purchased from Tocris Bioscience (Bristol, UK). IFNγ, IL-6, IL-1β, and Aspirin were used at concentrations of 2-50 ng/mL, 5 ng/mL, 1.25 ng/mL, and 1 mmol/L, respectively according to the previous reports [6, 8, 19] .
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