Osis morada 2x4 k ccd camera

A preparation of phage particles was centrifuged at 41,200× g for 120 min at 4 °C in a Beckman JA-20 rotor (Beckman Coulter, Fullerton, CA, USA) and suspended at about 10¹² PFU/mL in SM buffer; 10 μL of the concentrated phage suspension were let to adsorb on a carbon-coated matrix and then stained with 2% uranyl acetate for 15 s. The grid was subsequently washed twice with ddH₂O, air dried and observed by a FEI Tecnai 12 (FEI, Eindhoven, The Netherlands) TEM fitted with an Osis Morada 2X4 K CCD camera (Olympus, Shinjuku, Tokyo, Japan).

A bacteriophage suspension containing ≈10¹² PFU/ml, obtained by centrifugation at 25,000 × g for 60 minutes of 10 ml of the mixture from large-scale production of the phage, followed by resuspension of the pellet in 100 μl of SM buffer (pH = 7.5), was used for electron microscopy analysis. Preparations of bacteriophages particles were processed by standard negative stain^{38 (link)}, and observed by a FEI Tecnai 12 (FEI, Eindhoven, The Netherlands) TEM fitted with an Osis Morada 2X4 K CCD camera (Olympus, Shinjuku, Tokyo, Japan). In detail, 10 μl of the concentrated phage suspension were let to adsorb on a carbon-coated matrix and then stained with 2% uranyl acetate for 15 seconds. The grid was subsequently washed 2 times with ddH₂O, air dried and imaged by TEM.