Zinc sulfate heptahydrate

Zinc sulfate heptahydrate (ZnSO₄·7H₂O), potassium stannate trihydrate (K₂SnO₃·3H₂O), ammonium hydroxide (NH₃·H₂O), lithium chloride (LiCl), magnesium chloride (MgCl), potassium carbonate (K₂CO₃), magnesium nitrate (Mg(NO₃)₂), copper chloride (CuCl₂), sodium chloride (NaCl), potassium chloride (KCl), potassium nitrate (KNO₃), formaldehyde (HCHO), ethanol (C₂H₆O), acetone (C₃H₆O), and hydrogen peroxide (H₂O₂) were purchased from Sinopharm Chemical Reagent Co., Ltd., Beijing, China. All the reagents used in this experiment were of analytical grade.

Hericium erinaceus was purchased from the Yongding Ecological Farming Family Farm, Ningqiang County, Hanzhong City, Shaanxi Province.
Saliva α amylase (2000 U/g) was obtained from Omega, Cheyenne, WY, USA. Pepsin (250 U/g), pancreatic enzymes (10 U/g), fructose oligosaccharide, salt acid, concentrated sulfuric acid, sodium hydroxide, sodium chloride, ferrous sulfate heptahydrate, manganese sulfate, zinc sulfate heptahydrate, cobalt chloride hexahydrate, nickel chloride, copper sulfate pentahydrate disodium phosphate, potassium chloride, urea, magnesium chloride hexahydrate, calcium chloride dihydrate, metaphosphate, sodium sulfate, acetic acid standard, propionic acid standard, and butyric acid standard were obtained from Sinopharm Group Chemical Reagent Co., Ltd., Shanghai, China. All other chemical reagents were of analytical grade and procured from Aladdin Chemical Reagent Co., Ltd. (Shanghai, China).

All chemicals were of analytical grade and used as received without further purification. Zinc sulfate heptahydrate (ZnSO₄·7H₂O), sodium stannate trihydrate (Na₂SnO₃·3H₂O), and potassium permanganate (KMnO₄) were supplied by Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Bisphenol-A type EP was purchased from Shixian Chemical Industry Co., Ltd. A curing agent (diaminodiphenylmethane) and sodium hydroxide were purchased from Sinopharm Chemical Reagent Co., Ltd.

Heavy metal compounds, lead acetate ((CH₃COO)₂Pb·3H₂O, AR ≥ 99.5%), cadmium dichloride (CdCl₂·2.5H₂O, AR ≥ 99.0%), nickel dichloride (NiCl₂·6H₂O, AR ≥ 98.0%), manganese dichloride (MnCl₂·4H₂O, AR ≥ 99.0%), zinc sulfate heptahydrate (ZnSO₄·7H₂O, AR ≥ 99.5%), cupric sulfate (CuSO₄·5H₂O, AR ≥ 99.0%), and potassium dichromate (K₂Cr₂O₇, AR ≥ 99.8%) were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Methyl mercury chloride was purchased from Ehrenstorfer GmbH (affiliation, Germany). The biochemical and hematological analysis were performed by Hitachi 7600-110 autoanalyzer (Hitachi Ltd, Tokyo, Japan) and blood analyzer (Sysmex XT-1800i; Sysmex Co, Kobe, Japan), respectively. Water maze testing was performed using a MWM video analysis system (RD1101-MWM-G, Shanghai Mobiledatum Information Technology Co., Ltd., China). The monoclonal antibodies including p38, P-p38, JNK, p-JNK (phosphorylated JNK), NFκB, Nrf2, Akt, and GAPDH were obtained from Cell Signaling Technology (Danfoss, MA, USA). Sprague Dawley (SD) rats were obtained from Zhejiang Provincial Laboratory Animal Science Center (Hangzhou, Zhejiang, China).

Sodium acetate anhydrous, acetic acid, concentrated nitric acid, ammonia, anhydrous ethanol, copper sulfate pentahydrate, zinc sulfate heptahydrate, cadmium chloride dihydrate, lead chloride anhydrous, potassium ferricyanide, potassium chloride, sodium hydroxide, and hydrochloric acid were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). All the materials were used without further purification.
Acetate–sodium acetate buffer was prepared with 25 g/L sodium acetate and 10 mL/L acetic acid. The pH was adjusted to 4.8~5.0. For Fenton’s reagent, 1.52 mL of 8% ferrous sulfate was mixed with 0.2 mL of 30% hydrogen peroxide in 100 mL of distilled water, and the pH was adjusted to 2–4 with hydrochloric acid.
Pig farm wastewater samples were collected from a large-scale pig farm located in Huaian, Jiangsu Province, China, in June 2023. Wastewater is produced during the pig breeding process. The raw wastewater samples were collected before they were discharged into the wastewater treatment plant.

Epoxy resins (EP) were supplied by Hefei Jiangfeng Chemical Industry Co. (E-44, epoxy value: 0.44). Zinc sulfate heptahydrate (Zn_SO₄·7H₂O), 4,4-diamino-diphenyl methane (DDM) and sodium stannate trihydrate (Na₂SnO₃·3H₂O) were provided by Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Additionally, carbon nanotubes (CNTs) were provided by Chengdu Organic Chemicals Co., Ltd. (Chengdu, China).

Epoxy resins (EP) were supplied by Hefei Jiangfeng Chemical Industry Co. (E-44, epoxy value: 0.44). Zinc sulfate heptahydrate (Zn_SO₄·7H₂O), 4,4-diamino-diphenyl methane (DDM) and sodium stannate trihydrate (Na₂SnO₃·3H₂O) were provided by Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Additionally, carbon nanotubes (CNTs) were provided by Chengdu Organic Chemicals Co., Ltd. (Chengdu, China).