Microparticle chemiluminescence assay

Manufactured by Abbott

Microparticle chemiluminescence assay is a laboratory technique used to detect and quantify analytes in a sample. The assay utilizes microparticles coated with specific capture agents to bind the target analyte, followed by the addition of a chemiluminescent reagent to generate a measurable light signal proportional to the amount of analyte present.

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Lab products found in correlation

Sars cov 2 igg assay, by Abbott (2 mentions) I2000sr, by Abbott (1 mentions)

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Chemiluminescence assay (4 citations)

2 protocols using microparticle chemiluminescence assay

SARS-CoV-2 IgG Antibody Detection

Cited 1 time

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Detection of IgG antibody to SARS-CoV-2 N antigen was performed with a microparticle chemiluminescence assay (Abbott Laboratories) on the Abbott Architect i2000SR immunoassay analyzer. The EUA-approved Abbott SARS-CoV-2 IgG assay utilizes microparticles coated with SARS-CoV-2 N protein to capture N-specific IgG. Bound IgG was detected via addition of anti-human acridinium-labeled second-step antibody. Following a second wash step, pretrigger and trigger solutions were added, and a chemiluminescent reaction was detected and reported in relative light units (RLU). The RLU generated are reflective of the amount of antibody bound to the microparticles. The sample RLU were compared to the assay-specific calibrator RLU to generate an index value (S/C). Index values of ≥ 1.4 were considered positive. Sensitivity and specificity have been previously obtained for this assay (Table S3) (55 (link), 56 ).

Markmann A.J., Giallourou N., Bhowmik D.R., Hou Y.J., Lerner A., Martinez D.R., Premkumar L., Root H., van Duin D., Napravnik S., Graham S.D., Guerra Q., Raut R., Petropoulos C.J., Wrin T., Cornaby C., Schmitz J., Kuruc J., Weiss S., Park Y., Baric R., de Silva A.M., Margolis D.M, & Bartelt L.A. (2021). Sex Disparities and Neutralizing-Antibody Durability to SARS-CoV-2 Infection in Convalescent Individuals. mSphere, 6(4), e00275-21.

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SARS-CoV-2 IgG Antibody Detection Assay

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Detection of IgG antibody to SARS-CoV-2 N antigen was performed with a microparticle chemiluminescence assay (Abbott Laboratories) on the Abbott Architect i2000SR immunoassay analyzer. The EUA approved Abbott SARS-CoV-2 IgG assay utilizes microparticles coated with SARS-CoV-2 N protein to capture N specific IgG. Bound IgG was detected via addition of anti-human acridinium-labeled second-step antibody. Following a second wash step, pre-trigger and trigger solutions were added and a chemiluminescent reaction was detected and reported in relative light units (RLU). The RLU generated is reflective of the amount of antibody bound to the microparticles. The sample RLU was compared to the assay-specific calibrator RLU to generate an index value (S/C). Index values >/= 1.4 were considered positive. Sensitivity and specificity has been previously obtained for this assay (Supplementary Table 4) (37 (link), 38 ).

Markmann A.J., Giallourou N., Bhowmik D.R., Hou Y.J., Lerner A., Martinez D.R., Premkumar L., Root H., van Duin D., Napravnik S., Graham S.D., Guerra Q., Raut R., Petropoulos C.J., Wrin T., Cornaby C., Schmitz J., Kuruc J., Weiss S., Park Y., Baric R., de Silva A.M., Margolis D.M, & Bartelt L.A. (2021). Sex disparities and neutralizing antibody durability to SARS-CoV-2 infection in convalescent individuals. medRxiv.

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